Characteristics of in vivo and in vitro experimental models

S Kato, T Onaga, R Zabielski, Violetta Naughton (nee Lesniewska), P Guilloteau

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, in vivo and in vitro experimental models for the study of the exocrine pancreas in various animal species are reviewed. For the intact animal models, some anatomical details of the pancreatic duct system, which are important for the surgical preparation necessary for collection of pancreatic juice, are explained. Several techniques for collecting pancreatic juice are described, with their relevance to specific animal species. The advantages and disadvantages of various materials used for cannulas and catheters for collecting pancreatic juice are presented. For the vascular perfused models, some anatomical details of the vascular supply system are described, and the techniques used for preparation and perfusion, including the apparatus and composition of perfusates. As models for the study of cellular mechanisms, the characteristics of pancreatic segments, dispersed acinar cells and dispersed acini preparations are presented, as well as the techniques used. Systematic research into the functions of the exocrine pancreas, i.e. the pancreatic acini (synthesis and release of pancreatic enzymes and secretion of chloride-rich fluid) and ducts (secretion of bicarbonate-rich fluid), only began in the last hundred years using whole animal preparations. These preparations were necessary to study the secretory response of pancreatic juice to changes in various conditions: diets, stages of life, environments, diseases etc. and the role of pancreatic juice in the nutrition. The factors found to regulate pancreatic secretions in intact animals were investigated to see whether they acted directly on the pancreas or not. To simplify the interpretation by excluding interference from other organs, isolated perfused preparations or pancreatic segment preparations were developed. However, isolated pancreas and pancreatic segments still contain the endocrine portion, which regulates exocrine functions, as later became known. To study direct effects on acinar cells, preparations of dispersed single acinar cells and acini were developed. The development of these experimental models led to significant progress in animal and human pancreatology. The purpose of this chapter is to review and characterise experimental models for studying the functions of the exocrine pancreas.
LanguageEnglish
Title of host publicationBiology of the Pancreas in Growing Animals
EditorsStefan Grzegorz Pierzynowski, Romulald Zabielski
PublisherElsevier
Pages89-122
Volume28
ISBN (Print)0444502173
Publication statusPublished - 1999

Publication series

NameDEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES
PublisherElsevier Science Ltd

Fingerprint

Pancreatic Juice
Theoretical Models
Exocrine Pancreas
Acinar Cells
Blood Vessels
Pancreas
Anatomic Models
Fluids and Secretions
Pancreatic Ducts
Bicarbonates
Chlorides
Catheters
Animal Models
Perfusion
In Vitro Techniques
Diet
Enzymes
Research

Cite this

Kato, S., Onaga, T., Zabielski, R., Naughton (nee Lesniewska), V., & Guilloteau, P. (1999). Characteristics of in vivo and in vitro experimental models. In S. G. Pierzynowski, & R. Zabielski (Eds.), Biology of the Pancreas in Growing Animals (Vol. 28, pp. 89-122). (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES). Elsevier.
Kato, S ; Onaga, T ; Zabielski, R ; Naughton (nee Lesniewska), Violetta ; Guilloteau, P. / Characteristics of in vivo and in vitro experimental models. Biology of the Pancreas in Growing Animals. editor / Stefan Grzegorz Pierzynowski ; Romulald Zabielski. Vol. 28 Elsevier, 1999. pp. 89-122 (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).
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abstract = "In this chapter, in vivo and in vitro experimental models for the study of the exocrine pancreas in various animal species are reviewed. For the intact animal models, some anatomical details of the pancreatic duct system, which are important for the surgical preparation necessary for collection of pancreatic juice, are explained. Several techniques for collecting pancreatic juice are described, with their relevance to specific animal species. The advantages and disadvantages of various materials used for cannulas and catheters for collecting pancreatic juice are presented. For the vascular perfused models, some anatomical details of the vascular supply system are described, and the techniques used for preparation and perfusion, including the apparatus and composition of perfusates. As models for the study of cellular mechanisms, the characteristics of pancreatic segments, dispersed acinar cells and dispersed acini preparations are presented, as well as the techniques used. Systematic research into the functions of the exocrine pancreas, i.e. the pancreatic acini (synthesis and release of pancreatic enzymes and secretion of chloride-rich fluid) and ducts (secretion of bicarbonate-rich fluid), only began in the last hundred years using whole animal preparations. These preparations were necessary to study the secretory response of pancreatic juice to changes in various conditions: diets, stages of life, environments, diseases etc. and the role of pancreatic juice in the nutrition. The factors found to regulate pancreatic secretions in intact animals were investigated to see whether they acted directly on the pancreas or not. To simplify the interpretation by excluding interference from other organs, isolated perfused preparations or pancreatic segment preparations were developed. However, isolated pancreas and pancreatic segments still contain the endocrine portion, which regulates exocrine functions, as later became known. To study direct effects on acinar cells, preparations of dispersed single acinar cells and acini were developed. The development of these experimental models led to significant progress in animal and human pancreatology. The purpose of this chapter is to review and characterise experimental models for studying the functions of the exocrine pancreas.",
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Kato, S, Onaga, T, Zabielski, R, Naughton (nee Lesniewska), V & Guilloteau, P 1999, Characteristics of in vivo and in vitro experimental models. in SG Pierzynowski & R Zabielski (eds), Biology of the Pancreas in Growing Animals. vol. 28, DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES, Elsevier, pp. 89-122.

Characteristics of in vivo and in vitro experimental models. / Kato, S; Onaga, T; Zabielski, R; Naughton (nee Lesniewska), Violetta; Guilloteau, P.

Biology of the Pancreas in Growing Animals. ed. / Stefan Grzegorz Pierzynowski; Romulald Zabielski. Vol. 28 Elsevier, 1999. p. 89-122 (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Characteristics of in vivo and in vitro experimental models

AU - Kato, S

AU - Onaga, T

AU - Zabielski, R

AU - Naughton (nee Lesniewska), Violetta

AU - Guilloteau, P

N1 - Satellite Symposium on Biology of the Pancreas in Growing Animals at the 50th Annual Meeting of the European-Association-for-Animal-Production, ZURICH, SWITZERLAND, AUG, 1999

PY - 1999

Y1 - 1999

N2 - In this chapter, in vivo and in vitro experimental models for the study of the exocrine pancreas in various animal species are reviewed. For the intact animal models, some anatomical details of the pancreatic duct system, which are important for the surgical preparation necessary for collection of pancreatic juice, are explained. Several techniques for collecting pancreatic juice are described, with their relevance to specific animal species. The advantages and disadvantages of various materials used for cannulas and catheters for collecting pancreatic juice are presented. For the vascular perfused models, some anatomical details of the vascular supply system are described, and the techniques used for preparation and perfusion, including the apparatus and composition of perfusates. As models for the study of cellular mechanisms, the characteristics of pancreatic segments, dispersed acinar cells and dispersed acini preparations are presented, as well as the techniques used. Systematic research into the functions of the exocrine pancreas, i.e. the pancreatic acini (synthesis and release of pancreatic enzymes and secretion of chloride-rich fluid) and ducts (secretion of bicarbonate-rich fluid), only began in the last hundred years using whole animal preparations. These preparations were necessary to study the secretory response of pancreatic juice to changes in various conditions: diets, stages of life, environments, diseases etc. and the role of pancreatic juice in the nutrition. The factors found to regulate pancreatic secretions in intact animals were investigated to see whether they acted directly on the pancreas or not. To simplify the interpretation by excluding interference from other organs, isolated perfused preparations or pancreatic segment preparations were developed. However, isolated pancreas and pancreatic segments still contain the endocrine portion, which regulates exocrine functions, as later became known. To study direct effects on acinar cells, preparations of dispersed single acinar cells and acini were developed. The development of these experimental models led to significant progress in animal and human pancreatology. The purpose of this chapter is to review and characterise experimental models for studying the functions of the exocrine pancreas.

AB - In this chapter, in vivo and in vitro experimental models for the study of the exocrine pancreas in various animal species are reviewed. For the intact animal models, some anatomical details of the pancreatic duct system, which are important for the surgical preparation necessary for collection of pancreatic juice, are explained. Several techniques for collecting pancreatic juice are described, with their relevance to specific animal species. The advantages and disadvantages of various materials used for cannulas and catheters for collecting pancreatic juice are presented. For the vascular perfused models, some anatomical details of the vascular supply system are described, and the techniques used for preparation and perfusion, including the apparatus and composition of perfusates. As models for the study of cellular mechanisms, the characteristics of pancreatic segments, dispersed acinar cells and dispersed acini preparations are presented, as well as the techniques used. Systematic research into the functions of the exocrine pancreas, i.e. the pancreatic acini (synthesis and release of pancreatic enzymes and secretion of chloride-rich fluid) and ducts (secretion of bicarbonate-rich fluid), only began in the last hundred years using whole animal preparations. These preparations were necessary to study the secretory response of pancreatic juice to changes in various conditions: diets, stages of life, environments, diseases etc. and the role of pancreatic juice in the nutrition. The factors found to regulate pancreatic secretions in intact animals were investigated to see whether they acted directly on the pancreas or not. To simplify the interpretation by excluding interference from other organs, isolated perfused preparations or pancreatic segment preparations were developed. However, isolated pancreas and pancreatic segments still contain the endocrine portion, which regulates exocrine functions, as later became known. To study direct effects on acinar cells, preparations of dispersed single acinar cells and acini were developed. The development of these experimental models led to significant progress in animal and human pancreatology. The purpose of this chapter is to review and characterise experimental models for studying the functions of the exocrine pancreas.

M3 - Chapter

SN - 0444502173

VL - 28

T3 - DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES

SP - 89

EP - 122

BT - Biology of the Pancreas in Growing Animals

A2 - Pierzynowski, Stefan Grzegorz

A2 - Zabielski, Romulald

PB - Elsevier

ER -

Kato S, Onaga T, Zabielski R, Naughton (nee Lesniewska) V, Guilloteau P. Characteristics of in vivo and in vitro experimental models. In Pierzynowski SG, Zabielski R, editors, Biology of the Pancreas in Growing Animals. Vol. 28. Elsevier. 1999. p. 89-122. (DEVELOPMENTS IN ANIMAL AND VETERINARY SCIENCES).