Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer

Scott Funston, Konstantina Tsaousi, Michelle Rudden, Thomas Smyth, Paul Stevenson, R Marchant, Ibrahim M Banat

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture.Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.
LanguageEnglish
Pages7945-7956
JournalAppl Microbiol Biotechnol
Volume100
DOIs
Publication statusPublished - 5 May 2016

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Burkholderia thailandensis
concentration of production
rhamnolipids
biomass
cells
bioreactors
Gram-negative bacteria
Thailand
biomass production
temperature
quantitative polymerase chain reaction
genes
fermentation
biosynthesis

Keywords

  • Biosurfactants . Rhamnolipids . Burkholderia thailandensis . Gene expression . Di-rhamnolipids . qPCR

Cite this

Funston, Scott ; Tsaousi, Konstantina ; Rudden, Michelle ; Smyth, Thomas ; Stevenson, Paul ; Marchant, R ; Banat, Ibrahim M. / Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer. In: Appl Microbiol Biotechnol. 2016 ; Vol. 100. pp. 7945-7956.
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Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer. / Funston, Scott; Tsaousi, Konstantina; Rudden, Michelle; Smyth, Thomas; Stevenson, Paul; Marchant, R; Banat, Ibrahim M.

In: Appl Microbiol Biotechnol, Vol. 100, 05.05.2016, p. 7945-7956.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer

AU - Funston, Scott

AU - Tsaousi, Konstantina

AU - Rudden, Michelle

AU - Smyth, Thomas

AU - Stevenson, Paul

AU - Marchant, R

AU - Banat, Ibrahim M

PY - 2016/5/5

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N2 - Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture.Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.

AB - Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture.Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.

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U2 - 10.1007/s00253-016-7564-y

DO - 10.1007/s00253-016-7564-y

M3 - Article

VL - 100

SP - 7945

EP - 7956

JO - Applied Microbiology and Biotechnology

T2 - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

ER -