Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation

JL Creelan, AJ Bjourson, BM Meehan, SJ McCullough

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used to isolate unique fragments of the genome of an abortifacient strain (S26/3) of C. psittaci. One S26/3 strain-specific sequence identified was shown to encode a putative helicase which is repeated throughout the EAE genome. The labelled strain-specific helicase gene fragment was used in a dot-blot hybridisation test for the detection of EAE DNA in ovine placental samples. We report the identification of C. psittaci S26/3 strain-specific sequences with potential as diagnostic probes for the detection of EAE. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
LanguageEnglish
Pages17-25
JournalFems Microbiology Letters
Volume171
Issue number1
Publication statusPublished - Feb 1999

Fingerprint

Abortifacient Agents
Chlamydophila psittaci
Sheep
Genome
Placenta
DNA
Genes

Cite this

@article{978e0807617545bba657390212b3ef45,
title = "Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation",
abstract = "Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used to isolate unique fragments of the genome of an abortifacient strain (S26/3) of C. psittaci. One S26/3 strain-specific sequence identified was shown to encode a putative helicase which is repeated throughout the EAE genome. The labelled strain-specific helicase gene fragment was used in a dot-blot hybridisation test for the detection of EAE DNA in ovine placental samples. We report the identification of C. psittaci S26/3 strain-specific sequences with potential as diagnostic probes for the detection of EAE. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
author = "JL Creelan and AJ Bjourson and BM Meehan and SJ McCullough",
year = "1999",
month = "2",
language = "English",
volume = "171",
pages = "17--25",
journal = "Fems Microbiology Letters",
issn = "1574-6968",
number = "1",

}

Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation. / Creelan, JL; Bjourson, AJ; Meehan, BM; McCullough, SJ.

In: Fems Microbiology Letters, Vol. 171, No. 1, 02.1999, p. 17-25.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation

AU - Creelan, JL

AU - Bjourson, AJ

AU - Meehan, BM

AU - McCullough, SJ

PY - 1999/2

Y1 - 1999/2

N2 - Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used to isolate unique fragments of the genome of an abortifacient strain (S26/3) of C. psittaci. One S26/3 strain-specific sequence identified was shown to encode a putative helicase which is repeated throughout the EAE genome. The labelled strain-specific helicase gene fragment was used in a dot-blot hybridisation test for the detection of EAE DNA in ovine placental samples. We report the identification of C. psittaci S26/3 strain-specific sequences with potential as diagnostic probes for the detection of EAE. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used to isolate unique fragments of the genome of an abortifacient strain (S26/3) of C. psittaci. One S26/3 strain-specific sequence identified was shown to encode a putative helicase which is repeated throughout the EAE genome. The labelled strain-specific helicase gene fragment was used in a dot-blot hybridisation test for the detection of EAE DNA in ovine placental samples. We report the identification of C. psittaci S26/3 strain-specific sequences with potential as diagnostic probes for the detection of EAE. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

M3 - Article

VL - 171

SP - 17

EP - 25

JO - Fems Microbiology Letters

T2 - Fems Microbiology Letters

JF - Fems Microbiology Letters

SN - 1574-6968

SN - 0378-1097

IS - 1

ER -