Abstract
Oxazepam has been subjected to controlled degradation at 100 degrees C for 3h in 0.5M HCl and 0.5M NaOH. Following neutralisation of the degradation mixture and removal of salts by solid-phase extraction (SPE), isocratic high-performance liquid chromatography/mass spectrometry (HPLC/MS) using water/methanol (25:75 v/v) as the mobile phase was carried out using a flow diverter to collect fractions prior to their characterisation by electrospray ionisation multi-stage mass spectrometry (ESI-MSn) and proposal of the corresponding fragmentation patterns. The elemental compositions of the degradation products and their MS fragments were evaluated using electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS) which was then used to support the proposed fragmentation patterns. Copyright (C) 2010 John Wiley & Sons, Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 651-658 |
| Journal | Rapid Communications in Mass Spectrometry |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 2010 |
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Smyth, T. J. P., Rodriguez Robledo, V., & Smyth, F. (2010). Characterisation of oxazepam degradation products by high-performance liquid chromatography/electrospray ionisation mass spectrometry and electrospray ionisation quadrupole time-of-flight tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 24(5), 651-658. https://doi.org/10.1002/rcm.4433