Abstract
| Language | English |
|---|---|
| Pages | 651-658 |
| Journal | Rapid Communications in Mass Spectrometry |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 2010 |
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Characterisation of oxazepam degradation products by high-performance liquid chromatography/electrospray ionisation mass spectrometry and electrospray ionisation quadrupole time-of-flight tandem mass spectrometry. / Smyth, Thomas J. P.; Rodriguez Robledo, Virginia; Smyth, Franklin.
In: Rapid Communications in Mass Spectrometry, Vol. 24, No. 5, 03.2010, p. 651-658.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterisation of oxazepam degradation products by high-performance liquid chromatography/electrospray ionisation mass spectrometry and electrospray ionisation quadrupole time-of-flight tandem mass spectrometry
AU - Smyth, Thomas J. P.
AU - Rodriguez Robledo, Virginia
AU - Smyth, Franklin
PY - 2010/3
Y1 - 2010/3
N2 - Oxazepam has been subjected to controlled degradation at 100 degrees C for 3h in 0.5M HCl and 0.5M NaOH. Following neutralisation of the degradation mixture and removal of salts by solid-phase extraction (SPE), isocratic high-performance liquid chromatography/mass spectrometry (HPLC/MS) using water/methanol (25:75 v/v) as the mobile phase was carried out using a flow diverter to collect fractions prior to their characterisation by electrospray ionisation multi-stage mass spectrometry (ESI-MSn) and proposal of the corresponding fragmentation patterns. The elemental compositions of the degradation products and their MS fragments were evaluated using electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS) which was then used to support the proposed fragmentation patterns. Copyright (C) 2010 John Wiley & Sons, Ltd.
AB - Oxazepam has been subjected to controlled degradation at 100 degrees C for 3h in 0.5M HCl and 0.5M NaOH. Following neutralisation of the degradation mixture and removal of salts by solid-phase extraction (SPE), isocratic high-performance liquid chromatography/mass spectrometry (HPLC/MS) using water/methanol (25:75 v/v) as the mobile phase was carried out using a flow diverter to collect fractions prior to their characterisation by electrospray ionisation multi-stage mass spectrometry (ESI-MSn) and proposal of the corresponding fragmentation patterns. The elemental compositions of the degradation products and their MS fragments were evaluated using electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS) which was then used to support the proposed fragmentation patterns. Copyright (C) 2010 John Wiley & Sons, Ltd.
U2 - 10.1002/rcm.4433
DO - 10.1002/rcm.4433
M3 - Article
VL - 24
SP - 651
EP - 658
JO - Rapid Communications in Mass Spectrometry
T2 - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
SN - 0951-4198
IS - 5
ER -