Characterisation of a novel thermotolerant yeast, Kluyveromyces marxianus var marxianus: development of an ethanol fermentation process

Catherine Hack, Roger Marchant

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Abstract

The fermentation characteristics of the novel, thermotolerant, isolate Kloyveromyces marxianus var marxianus were determined to evaluate its aptitude for use in an ethanol production process, Sustainable growth was not observed under anaerobic conditions, even in the presence of unsaturated fatty acid and sterol, A maximum ethanol concentration of 40 g L-1 was produced at 45 degrees C, with an initial specific ethanol production rate of 1.7 g g(-1) h(-1). This was observed at ethanol concentrations below 8 g L-1 and under oxygen-limited conditions, The low ethanol tolerance and low growth under oxygen-limited conditions required for ethanol production implied that a simple continuous process was not feasible with this yeast strain. Improved productivity was achieved through recycling biomass into the fermenter, indicating that utilising an effective cell retention method such as cell recycle or immobilisation, could lead to the development of a viable industrial process using this novel yeast strain.
LanguageEnglish
Pages323-327
JournalJournal of Industrial Microbiology and Biotechnology
Volume20
Issue number6
Publication statusPublished - Jun 1998

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Kluyveromyces marxianus
ethanol fermentation
ethanol production
ethanol
yeasts
oxygen
fermenters
unsaturated fatty acids
anaerobic conditions
sterols
recycling
fermentation
cells
biomass
methodology

Cite this

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abstract = "The fermentation characteristics of the novel, thermotolerant, isolate Kloyveromyces marxianus var marxianus were determined to evaluate its aptitude for use in an ethanol production process, Sustainable growth was not observed under anaerobic conditions, even in the presence of unsaturated fatty acid and sterol, A maximum ethanol concentration of 40 g L-1 was produced at 45 degrees C, with an initial specific ethanol production rate of 1.7 g g(-1) h(-1). This was observed at ethanol concentrations below 8 g L-1 and under oxygen-limited conditions, The low ethanol tolerance and low growth under oxygen-limited conditions required for ethanol production implied that a simple continuous process was not feasible with this yeast strain. Improved productivity was achieved through recycling biomass into the fermenter, indicating that utilising an effective cell retention method such as cell recycle or immobilisation, could lead to the development of a viable industrial process using this novel yeast strain.",
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AU - Marchant, Roger

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N2 - The fermentation characteristics of the novel, thermotolerant, isolate Kloyveromyces marxianus var marxianus were determined to evaluate its aptitude for use in an ethanol production process, Sustainable growth was not observed under anaerobic conditions, even in the presence of unsaturated fatty acid and sterol, A maximum ethanol concentration of 40 g L-1 was produced at 45 degrees C, with an initial specific ethanol production rate of 1.7 g g(-1) h(-1). This was observed at ethanol concentrations below 8 g L-1 and under oxygen-limited conditions, The low ethanol tolerance and low growth under oxygen-limited conditions required for ethanol production implied that a simple continuous process was not feasible with this yeast strain. Improved productivity was achieved through recycling biomass into the fermenter, indicating that utilising an effective cell retention method such as cell recycle or immobilisation, could lead to the development of a viable industrial process using this novel yeast strain.

AB - The fermentation characteristics of the novel, thermotolerant, isolate Kloyveromyces marxianus var marxianus were determined to evaluate its aptitude for use in an ethanol production process, Sustainable growth was not observed under anaerobic conditions, even in the presence of unsaturated fatty acid and sterol, A maximum ethanol concentration of 40 g L-1 was produced at 45 degrees C, with an initial specific ethanol production rate of 1.7 g g(-1) h(-1). This was observed at ethanol concentrations below 8 g L-1 and under oxygen-limited conditions, The low ethanol tolerance and low growth under oxygen-limited conditions required for ethanol production implied that a simple continuous process was not feasible with this yeast strain. Improved productivity was achieved through recycling biomass into the fermenter, indicating that utilising an effective cell retention method such as cell recycle or immobilisation, could lead to the development of a viable industrial process using this novel yeast strain.

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EP - 327

JO - Journal of Industrial Microbiology and Biotechnology

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