Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry

Stephen McClean, R. C. Robinson, C. Shaw, Franklin Smyth

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Abstract

The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'-trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-methoxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MS(n) experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (M(+)) and 5-MeODMT (MH(+)). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 microg per mg of skin secretion, respectively.
LanguageEnglish
Pages346-354
JournalRapid Communications in Mass Spectrometry
Volume16
Issue number5
Publication statusPublished - 2002

Fingerprint

Indole Alkaloids
Electrospray ionization
Mass spectrometry
Serotonin
Skin
Ions
Bufotenin
Methoxydimethyltryptamines
Mass spectrometers
serotonin drug combination creatinine sulfate
Experiments

Keywords

  • Alkaloids/analysis
  • Animals
  • Anura
  • Chromatography
  • Liquid
  • Indicators and Reagents
  • Indoles/analysis
  • Serotonin/analysis
  • Skin/chemistry
  • Spectrometry
  • Mass
  • Electrospray Ionization
  • Tryptamines/analysis

Cite this

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title = "Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry",
abstract = "The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'-trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-methoxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MS(n) experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (M(+)) and 5-MeODMT (MH(+)). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 microg per mg of skin secretion, respectively.",
keywords = "Alkaloids/analysis, Animals, Anura, Chromatography, Liquid, Indicators and Reagents, Indoles/analysis, Serotonin/analysis, Skin/chemistry, Spectrometry, Mass, Electrospray Ionization, Tryptamines/analysis",
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volume = "16",
pages = "346--354",
journal = "Rapid Communications in Mass Spectrometry",
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TY - JOUR

T1 - Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry

AU - McClean, Stephen

AU - Robinson, R. C.

AU - Shaw, C.

AU - Smyth, Franklin

N1 - LR: 20031114; PUBM: Print; CI: Copyright 2002; JID: 8802365; 0 (Alkaloids); 0 (Indicators and Reagents); 0 (Indoles); 0 (Tryptamines); 50-67-9 (Serotonin); ppublish

PY - 2002

Y1 - 2002

N2 - The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'-trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-methoxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MS(n) experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (M(+)) and 5-MeODMT (MH(+)). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 microg per mg of skin secretion, respectively.

AB - The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'-trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-methoxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MS(n) experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (M(+)) and 5-MeODMT (MH(+)). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 microg per mg of skin secretion, respectively.

KW - Alkaloids/analysis

KW - Animals

KW - Anura

KW - Chromatography

KW - Liquid

KW - Indicators and Reagents

KW - Indoles/analysis

KW - Serotonin/analysis

KW - Skin/chemistry

KW - Spectrometry

KW - Mass

KW - Electrospray Ionization

KW - Tryptamines/analysis

M3 - Article

VL - 16

SP - 346

EP - 354

JO - Rapid Communications in Mass Spectrometry

T2 - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 5

ER -