Cell-cycle delay is induced in cells of a U937 promonocytic cell line by low-intensity light irradiation at 660 nm

KM Joyce, Stephen Downes, BM Hannigan

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Visible-light irradiation (VLI) at 660 nm and 11.5 J/cm(2) inhibits proliferation of cells of the U937 promonocytic cell line, as monitored by autoradiographical analysis. The S-phase cell population is reduced at 6 h post-radiation treatment. Flow cytometric analysis confirms this, and also shows that light irradiation of cells induces a statistically significant increase in G2/M cells at 6 h post-radiation treatment. It has been postulated that VLI at 660 nm can alter cell-cycle progression by affecting intracellular concentrations of ions, in particular pH and calcium. However, no significant effects of light irradiation on these intracellular ions have been observed. These effects of VLI are not a consequence of radiation-induced DNA strand breaks, therefore events other than direct DNA damage are involved. These findings demonstrate a direct photobiological effect of VLI at 660 nm on the cell cycle, and indicate a previously unsuspected mechanism for the induction of cell-cycle delay that is neither a result of changes in the concentration of intracellular ions nor initiated by DNA strand breaks. (C) 1999 Elsevier Science S.A. All rights reserved.
    LanguageEnglish
    Pages117-122
    JournalJOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
    Volume52
    Issue number1-3
    Publication statusPublished - Sep 1999

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    cultured cells
    luminous intensity
    cycles
    irradiation
    cells
    deoxyribonucleic acid
    strands
    radiation
    ions
    progressions
    calcium
    induction
    damage

    Cite this

    @article{a84df571a5f243ba970d158501c2a6e4,
    title = "Cell-cycle delay is induced in cells of a U937 promonocytic cell line by low-intensity light irradiation at 660 nm",
    abstract = "Visible-light irradiation (VLI) at 660 nm and 11.5 J/cm(2) inhibits proliferation of cells of the U937 promonocytic cell line, as monitored by autoradiographical analysis. The S-phase cell population is reduced at 6 h post-radiation treatment. Flow cytometric analysis confirms this, and also shows that light irradiation of cells induces a statistically significant increase in G2/M cells at 6 h post-radiation treatment. It has been postulated that VLI at 660 nm can alter cell-cycle progression by affecting intracellular concentrations of ions, in particular pH and calcium. However, no significant effects of light irradiation on these intracellular ions have been observed. These effects of VLI are not a consequence of radiation-induced DNA strand breaks, therefore events other than direct DNA damage are involved. These findings demonstrate a direct photobiological effect of VLI at 660 nm on the cell cycle, and indicate a previously unsuspected mechanism for the induction of cell-cycle delay that is neither a result of changes in the concentration of intracellular ions nor initiated by DNA strand breaks. (C) 1999 Elsevier Science S.A. All rights reserved.",
    author = "KM Joyce and Stephen Downes and BM Hannigan",
    year = "1999",
    month = "9",
    language = "English",
    volume = "52",
    pages = "117--122",
    journal = "Journal of Photochemistry and Photobiology B: Biology",
    issn = "1011-1344",
    publisher = "Elsevier",
    number = "1-3",

    }

    Cell-cycle delay is induced in cells of a U937 promonocytic cell line by low-intensity light irradiation at 660 nm. / Joyce, KM; Downes, Stephen; Hannigan, BM.

    In: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, Vol. 52, No. 1-3, 09.1999, p. 117-122.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Cell-cycle delay is induced in cells of a U937 promonocytic cell line by low-intensity light irradiation at 660 nm

    AU - Joyce, KM

    AU - Downes, Stephen

    AU - Hannigan, BM

    PY - 1999/9

    Y1 - 1999/9

    N2 - Visible-light irradiation (VLI) at 660 nm and 11.5 J/cm(2) inhibits proliferation of cells of the U937 promonocytic cell line, as monitored by autoradiographical analysis. The S-phase cell population is reduced at 6 h post-radiation treatment. Flow cytometric analysis confirms this, and also shows that light irradiation of cells induces a statistically significant increase in G2/M cells at 6 h post-radiation treatment. It has been postulated that VLI at 660 nm can alter cell-cycle progression by affecting intracellular concentrations of ions, in particular pH and calcium. However, no significant effects of light irradiation on these intracellular ions have been observed. These effects of VLI are not a consequence of radiation-induced DNA strand breaks, therefore events other than direct DNA damage are involved. These findings demonstrate a direct photobiological effect of VLI at 660 nm on the cell cycle, and indicate a previously unsuspected mechanism for the induction of cell-cycle delay that is neither a result of changes in the concentration of intracellular ions nor initiated by DNA strand breaks. (C) 1999 Elsevier Science S.A. All rights reserved.

    AB - Visible-light irradiation (VLI) at 660 nm and 11.5 J/cm(2) inhibits proliferation of cells of the U937 promonocytic cell line, as monitored by autoradiographical analysis. The S-phase cell population is reduced at 6 h post-radiation treatment. Flow cytometric analysis confirms this, and also shows that light irradiation of cells induces a statistically significant increase in G2/M cells at 6 h post-radiation treatment. It has been postulated that VLI at 660 nm can alter cell-cycle progression by affecting intracellular concentrations of ions, in particular pH and calcium. However, no significant effects of light irradiation on these intracellular ions have been observed. These effects of VLI are not a consequence of radiation-induced DNA strand breaks, therefore events other than direct DNA damage are involved. These findings demonstrate a direct photobiological effect of VLI at 660 nm on the cell cycle, and indicate a previously unsuspected mechanism for the induction of cell-cycle delay that is neither a result of changes in the concentration of intracellular ions nor initiated by DNA strand breaks. (C) 1999 Elsevier Science S.A. All rights reserved.

    M3 - Article

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    JO - Journal of Photochemistry and Photobiology B: Biology

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