TY - JOUR
T1 - Cell biology at the interface of nanobiosensors and microfluidics
AU - Bhalla, Nikhil
AU - Chiang, Hung Ju
AU - Shen, Amy Q.
PY - 2018/11/15
Y1 - 2018/11/15
N2 - It is at the nanometer scale where biology, chemistry, and material science converge. With recent progresses in nanotechnology, research in cell biology has gained enormous interest from a growing multidisciplinary community of scientists in academia and industry. Many efforts have been made to discover cures for diseases through advancements in nanobiosensors and microfluidics. In this chapter, we give a general introduction to nanobiosensors and microfluidics technology, present key developments in the field, and illustrate the breadth of microfluidics-based sensing strategies available for cell biology. We also provide protocols for fabricating localized surface plasmon resonance (LSPR)-based nanobiosensors, and for integration of LSPR with a microfluidic device. Finally, we highlight applications and challenges associated with the use of nanobiosensing and microfluidics technology. This chapter should provide a solid platform for cell biologists to develop simple microfluidic LSPR chips for routine cell-based assays.
AB - It is at the nanometer scale where biology, chemistry, and material science converge. With recent progresses in nanotechnology, research in cell biology has gained enormous interest from a growing multidisciplinary community of scientists in academia and industry. Many efforts have been made to discover cures for diseases through advancements in nanobiosensors and microfluidics. In this chapter, we give a general introduction to nanobiosensors and microfluidics technology, present key developments in the field, and illustrate the breadth of microfluidics-based sensing strategies available for cell biology. We also provide protocols for fabricating localized surface plasmon resonance (LSPR)-based nanobiosensors, and for integration of LSPR with a microfluidic device. Finally, we highlight applications and challenges associated with the use of nanobiosensing and microfluidics technology. This chapter should provide a solid platform for cell biologists to develop simple microfluidic LSPR chips for routine cell-based assays.
KW - Bioassays
KW - Biosensors
KW - Cell biology
KW - LSPR
KW - Microfluidics
KW - Nanoplasmonics
UR - http://www.scopus.com/inward/record.url?scp=85056448750&partnerID=8YFLogxK
U2 - 10.1016/bs.mcb.2018.09.009
DO - 10.1016/bs.mcb.2018.09.009
M3 - Article
C2 - 30473070
AN - SCOPUS:85056448750
SN - 0091-679X
SP - 203
EP - 227
JO - Methods in Cell Biology
JF - Methods in Cell Biology
ER -