Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

Danyelle K. F. Santos, Ana H. M. Resende, Darne G. de Almeida, Rita de Cássia F. Soares da Silva, Raquel D. Rufino, Juliana M. Luna, Ibrahim M Banat, Leonie A. Sarubbo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aim of the present study was to investigate the potential application of the biosurfactant from Candida lipolytica grown in low-cost substrates, which has previously been produced and characterized under optimized conditions as an adjunct material to enhance the remediation processes of hydrophobic pollutants and heavy metals generated by the oil industry and propose the formulation of a safe and stable remediation agent. In tests carried out with seawater, the crude biosurfactant demonstrated 80% oil spreading efficiency. The dispersion rate was 50% for the biosurfactant at a concentration twice that of the CMC. The biosurfactant removed 70% of motor oil from contaminated cotton cloth in detergency tests. The crude biosurfactant also removed 30–40% of Cu and Pb from standard sand, while the isolated biosurfactant removed ~30% of the heavy metals. The conductivity of solutions containing Cd and Pb was sharply reduced after biosurfactants' addition. A product was prepared through adding 0.2% potassium sorbate as preservative and tested over 120 days. The formulated biosurfactant was analyzed for emulsification and surface tension under different pH values, temperatures, and salt concentrations and tested for toxicity against the fish Poecilia vivipara. The results showed that the formulation had no toxicity and did not cause significant changes in the tensoactive capacity of the biomolecule while maintaining activity demonstrating suitability for potential future commercial product formulation.
LanguageEnglish
JournalFrontiers in Microbiology
Volume8
Issue number767
DOIs
Publication statusPublished - 1 May 2017

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bioremediation
remediation
heavy metal
toxicity
oil
preservative
surface tension
oil industry
cotton
conductivity
potassium
salt
seawater
substrate
sand
pollutant
fish
cost
temperature
product

Keywords

  • Biosurfactant
  • Candida lipolytica
  • animal fat
  • corn steep liquor
  • bioremediation
  • petroleum
  • heavy metals

Cite this

Santos, D. K. F., Resende, A. H. M., de Almeida, D. G., Soares da Silva, R. D. C. F., Rufino, R. D., Luna, J. M., ... Sarubbo, L. A. (2017). Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product. Frontiers in Microbiology, 8(767). https://doi.org/10.3389/fmicb.2017.00767
Santos, Danyelle K. F. ; Resende, Ana H. M. ; de Almeida, Darne G. ; Soares da Silva, Rita de Cássia F. ; Rufino, Raquel D. ; Luna, Juliana M. ; Banat, Ibrahim M ; Sarubbo, Leonie A. / Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. 767.
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Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product. / Santos, Danyelle K. F.; Resende, Ana H. M.; de Almeida, Darne G.; Soares da Silva, Rita de Cássia F.; Rufino, Raquel D.; Luna, Juliana M.; Banat, Ibrahim M; Sarubbo, Leonie A.

In: Frontiers in Microbiology, Vol. 8, No. 767, 01.05.2017.

Research output: Contribution to journalArticle

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T1 - Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

AU - Santos, Danyelle K. F.

AU - Resende, Ana H. M.

AU - de Almeida, Darne G.

AU - Soares da Silva, Rita de Cássia F.

AU - Rufino, Raquel D.

AU - Luna, Juliana M.

AU - Banat, Ibrahim M

AU - Sarubbo, Leonie A.

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AB - The aim of the present study was to investigate the potential application of the biosurfactant from Candida lipolytica grown in low-cost substrates, which has previously been produced and characterized under optimized conditions as an adjunct material to enhance the remediation processes of hydrophobic pollutants and heavy metals generated by the oil industry and propose the formulation of a safe and stable remediation agent. In tests carried out with seawater, the crude biosurfactant demonstrated 80% oil spreading efficiency. The dispersion rate was 50% for the biosurfactant at a concentration twice that of the CMC. The biosurfactant removed 70% of motor oil from contaminated cotton cloth in detergency tests. The crude biosurfactant also removed 30–40% of Cu and Pb from standard sand, while the isolated biosurfactant removed ~30% of the heavy metals. The conductivity of solutions containing Cd and Pb was sharply reduced after biosurfactants' addition. A product was prepared through adding 0.2% potassium sorbate as preservative and tested over 120 days. The formulated biosurfactant was analyzed for emulsification and surface tension under different pH values, temperatures, and salt concentrations and tested for toxicity against the fish Poecilia vivipara. The results showed that the formulation had no toxicity and did not cause significant changes in the tensoactive capacity of the biomolecule while maintaining activity demonstrating suitability for potential future commercial product formulation.

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KW - Candida lipolytica

KW - animal fat

KW - corn steep liquor

KW - bioremediation

KW - petroleum

KW - heavy metals

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JO - Frontiers in Microbiology

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SN - 1664-302X

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