Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay

Richard Owusu-Apenten; Hau Ying Ng

Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay

Richard Owusu-Apenten
, Hau Ying Ng

School of Biomedical Sciences

Research output: Non-textual form › Web publication/site

Abstract

A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).

Original language	English
Publication status	Published (in print/issue) - 20 Apr 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antioxidant capacity
microplate assays
FRAP
DPPH

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http://www.researchgate.net/publication/275212740_Calibrated_microplate_assays_for_total_antioxidant_capacity_using_DPPH_and_comparisons_with_the_FRAP_assay

Cite this

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title = "Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay",

abstract = "A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).",

keywords = "antioxidant capacity, microplate assays, FRAP, DPPH",

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year = "2015",

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day = "20",

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TY - ADVS

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AU - Owusu-Apenten, Richard

AU - Ng, Hau Ying

PY - 2015/4/20

Y1 - 2015/4/20

N2 - A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).

AB - A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).

KW - antioxidant capacity

KW - microplate assays

KW - FRAP

KW - DPPH

M3 - Web publication/site

ER -

Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay

Abstract

UN SDGs

Keywords

Access to Document

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Cite this