Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay

Richard Owusu-Apenten, Hau Ying Ng

Research output: Non-textual formWeb publication/site

Abstract

A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).
Original languageEnglish
Publication statusPublished - 20 Apr 2015

Keywords

  • antioxidant capacity
  • microplate assays
  • FRAP
  • DPPH

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