Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay

Richard Owusu-Apenten, Hau Ying Ng

Research output: Non-textual formWeb publication/site

Abstract

A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).
LanguageEnglish
Publication statusPublished - 20 Apr 2015

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antioxidants
spectrophotometers
assays
quality control
methodology

Keywords

  • antioxidant capacity
  • microplate assays
  • FRAP
  • DPPH

Cite this

Owusu-Apenten, Richard (Author) ; Ng, Hau Ying (Author). / Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay. [Web publication/site].
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abstract = "A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).",
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year = "2015",
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Calibrated microplate assays for total antioxidant capacity using DPPH and comparisons with the FRAP assay. Owusu-Apenten, Richard (Author); Ng, Hau Ying (Author). 2015.

Research output: Non-textual formWeb publication/site

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AU - Ng, Hau Ying

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N2 - A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).

AB - A microplate DPPH assay for antioxidants is described and subjected to a manual path-length correction to yield results comparable to measurements using 1-cm spectrophotometer. The described method is important for quality control for microplate assays and follows on from principles first illustrated in a previous paper (Bolanos de la Torre et al. 2015).

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KW - microplate assays

KW - FRAP

KW - DPPH

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