Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

TB Chen, DF Orr, AJ Bjourson, Stephen McClean, M O'Rourke, DG Hirst, PF Rao, C Shaw

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1547-1555
JournalPeptides
Volume23
Issue number9
Publication statusPublished - Sep 2002

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Kininogens
Thr(6)-bradykinin
Bradykinin
Anura
Complementary DNA
Skin
Open Reading Frames
Amino Acids
Amphibians
Amino Acid Sequence
Nucleotides
Peptides

Cite this

Chen, TB., Orr, DF., Bjourson, AJ., McClean, S., O'Rourke, M., Hirst, DG., ... Shaw, C. (2002). Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). Peptides, 23(9), 1547-1555.
Chen, TB ; Orr, DF ; Bjourson, AJ ; McClean, Stephen ; O'Rourke, M ; Hirst, DG ; Rao, PF ; Shaw, C. / Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). In: Peptides. 2002 ; Vol. 23, No. 9. pp. 1547-1555.
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Chen, TB, Orr, DF, Bjourson, AJ, McClean, S, O'Rourke, M, Hirst, DG, Rao, PF & Shaw, C 2002, 'Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)', Peptides, vol. 23, no. 9, pp. 1547-1555.

Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). / Chen, TB; Orr, DF; Bjourson, AJ; McClean, Stephen; O'Rourke, M; Hirst, DG; Rao, PF; Shaw, C.

In: Peptides, Vol. 23, No. 9, 09.2002, p. 1547-1555.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

AU - Chen, TB

AU - Orr, DF

AU - Bjourson, AJ

AU - McClean, Stephen

AU - O'Rourke, M

AU - Hirst, DG

AU - Rao, PF

AU - Shaw, C

PY - 2002/9

Y1 - 2002/9

N2 - Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.

AB - Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.

M3 - Article

VL - 23

SP - 1547

EP - 1555

JO - Peptides

JF - Peptides

SN - 0196-9781

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ER -