Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

TB Chen; DF Orr; AJ Bjourson; Stephen McClean; M O'Rourke; DG Hirst; PF Rao; C Shaw

Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

TB Chen, DF Orr, AJ Bjourson, Stephen McClean, M O'Rourke, DG Hirst, PF Rao, C Shaw

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Abstract

Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.

Original language	English
Pages (from-to)	1547-1555
Journal	Peptides
Volume	23
Issue number	9
Publication status	Published (in print/issue) - Sept 2002

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Chen, TB., Orr, DF., Bjourson, AJ., McClean, S., O'Rourke, M., Hirst, DG., Rao, PF., & Shaw, C. (2002). Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). Peptides, 23(9), 1547-1555. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0M-46NXNCX-2&_user=126978&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000010438&_version=1&_urlVersion=0&_userid=126978&md5=7320d53ff1bc5be630f96c603dd52786

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title = "Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)",

abstract = "Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.",

author = "TB Chen and DF Orr and AJ Bjourson and Stephen McClean and M O'Rourke and DG Hirst and PF Rao and C Shaw",

year = "2002",

month = sep,

language = "English",

volume = "23",

pages = "1547--1555",

journal = "Peptides",

publisher = "Elsevier",

number = "9",

}

Chen, TB, Orr, DF, Bjourson, AJ , McClean, S, O'Rourke, M, Hirst, DG, Rao, PF & Shaw, C 2002, 'Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)', Peptides, vol. 23, no. 9, pp. 1547-1555. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0M-46NXNCX-2&_user=126978&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000010438&_version=1&_urlVersion=0&_userid=126978&md5=7320d53ff1bc5be630f96c603dd52786>

TY - JOUR

T1 - Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

AU - Chen, TB

AU - Orr, DF

AU - Bjourson, AJ

AU - McClean, Stephen

AU - O'Rourke, M

AU - Hirst, DG

AU - Rao, PF

AU - Shaw, C

PY - 2002/9

Y1 - 2002/9

N2 - Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.

AB - Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.

M3 - Article

VL - 23

SP - 1547

EP - 1555

JO - Peptides

JF - Peptides

IS - 9

ER -

Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

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