Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)

TB Chen, DF Orr, AJ Bjourson, Stephen McClean, M O'Rourke, DG Hirst, PF Rao, C Shaw

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. (C) 2002 Elsevier Science Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1547-1555
JournalPeptides
Volume23
Issue number9
Publication statusPublished - Sep 2002

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