Bradykinin receptors in the zebrafish (Danio rerio)

Torun Bromée; J. Michael Conlon; Dan Larhammar

doi:10.1196/annals.1327.034

Bradykinin receptors in the zebrafish (Danio rerio)

Torun Bromée, J. Michael Conlon, Dan Larhammar

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Ligand interactions of a zebrafish bradykinin (BK) receptor expressed in vitro were characterized by measuring inositol phosphate accumulation. The ligands were analogues of zebrafish BK. Substitutions of Arg1, Gly4, Ser6, Pro7, Leu8, and Arg9 caused greatly reduced potency and maximum response. The Pro3 to Ala analogue had higher potency, but lower maximum response. These and other differences show that the zebrafish BK receptor has a ligand-interaction profile that is distinct from mammalian B1 and B2 receptors and from the previously characterized BK receptor in trout stomach. The results increase our understanding of the evolution of BK receptors and their ligands.

Original language	English
Pages (from-to)	246-248
Number of pages	3
Journal	Annals of the New York Academy of Sciences
Volume	1040
DOIs	https://doi.org/10.1196/annals.1327.034
Publication status	Published (in print/issue) - 2005

Keywords

Alanine
Bradykinin receptor
D-isomer
Inositol phosphates (IP)

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10.1196/annals.1327.034

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AU - Bromée, Torun

AU - Conlon, J. Michael

AU - Larhammar, Dan

PY - 2005

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AB - Ligand interactions of a zebrafish bradykinin (BK) receptor expressed in vitro were characterized by measuring inositol phosphate accumulation. The ligands were analogues of zebrafish BK. Substitutions of Arg1, Gly4, Ser6, Pro7, Leu8, and Arg9 caused greatly reduced potency and maximum response. The Pro3 to Ala analogue had higher potency, but lower maximum response. These and other differences show that the zebrafish BK receptor has a ligand-interaction profile that is distinct from mammalian B1 and B2 receptors and from the previously characterized BK receptor in trout stomach. The results increase our understanding of the evolution of BK receptors and their ligands.

KW - Alanine

KW - Bradykinin receptor

KW - D-isomer

KW - Inositol phosphates (IP)

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SN - 0077-8923

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EP - 248

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Bradykinin receptors in the zebrafish (Danio rerio)

Abstract

Keywords

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