Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice.

Parthsarathy V, Christopher McLaughlin, PA Harnedy, Philip J Allsopp, William Crowe, Emeir Mc Sorley, R Fitzgerald, Finbarr O'Harte

Research output: Contribution to journalArticle

Abstract

The antidiabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4L and Flavourzyme 500L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01-2.5 mg/ml) were tested in vitro for DPP-IV inhibition andinsulin and GLP-1 secretory activity from BRIN-BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose-dependent increase (4.2 to 5.3-fold, p<0.001) in insulin secretion from BRIN-BD11 cells and inhibited DPP-IV activity (IC50 1.18±0.04 and 1.21±0.04 mg/ml), respectively. The SGID sample produced a 1.3-fold (p<0.01) increase in GLP-1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n=8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0-120 min), p<0.05) and a consequent reduction in blood glucose concentration (p<0.01), after OGTT in mice versus controls. The BPH showed potent antidiabetic actions in cells and improved glucose tolerance in mice.
LanguageEnglish
Article numberxx
Pagesxx
JournalInternational Journal of Food Science and Technology
Volumexx
Issue numberxx
DOIs
Publication statusAccepted/In press - 10 Sep 2018

Fingerprint

glucagon-like peptide 1
Protein Hydrolysates
Glucagon-Like Peptide 1
protein hydrolysates
Glucose
Proteins
glucose
mice
Digestion
digestion
hypoglycemic agents
Insulin
Glucose Tolerance Test
Hypoglycemic Agents
Subtilisins
Capros aper
In Vitro Techniques
Muscle Proteins
subtilisin
insulin secretion

Keywords

  • boarfish protein hydrolysate
  • insulin secretion
  • Glucagon like peptide 1 (GLP-1)
  • dipeptidyl peptidase IV
  • glycaemic control

Cite this

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title = "Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice.",
abstract = "The antidiabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4L and Flavourzyme 500L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01-2.5 mg/ml) were tested in vitro for DPP-IV inhibition andinsulin and GLP-1 secretory activity from BRIN-BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose-dependent increase (4.2 to 5.3-fold, p<0.001) in insulin secretion from BRIN-BD11 cells and inhibited DPP-IV activity (IC50 1.18±0.04 and 1.21±0.04 mg/ml), respectively. The SGID sample produced a 1.3-fold (p<0.01) increase in GLP-1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n=8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0-120 min), p<0.05) and a consequent reduction in blood glucose concentration (p<0.01), after OGTT in mice versus controls. The BPH showed potent antidiabetic actions in cells and improved glucose tolerance in mice.",
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year = "2018",
month = "9",
day = "10",
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Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice. / V, Parthsarathy; McLaughlin, Christopher; Harnedy, PA; Allsopp, Philip J; Crowe, William; Mc Sorley, Emeir; Fitzgerald, R; O'Harte, Finbarr.

In: International Journal of Food Science and Technology, Vol. xx, No. xx, xx, 10.09.2018, p. xx.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice.

AU - V, Parthsarathy

AU - McLaughlin, Christopher

AU - Harnedy, PA

AU - Allsopp, Philip J

AU - Crowe, William

AU - Mc Sorley, Emeir

AU - Fitzgerald, R

AU - O'Harte, Finbarr

PY - 2018/9/10

Y1 - 2018/9/10

N2 - The antidiabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4L and Flavourzyme 500L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01-2.5 mg/ml) were tested in vitro for DPP-IV inhibition andinsulin and GLP-1 secretory activity from BRIN-BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose-dependent increase (4.2 to 5.3-fold, p<0.001) in insulin secretion from BRIN-BD11 cells and inhibited DPP-IV activity (IC50 1.18±0.04 and 1.21±0.04 mg/ml), respectively. The SGID sample produced a 1.3-fold (p<0.01) increase in GLP-1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n=8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0-120 min), p<0.05) and a consequent reduction in blood glucose concentration (p<0.01), after OGTT in mice versus controls. The BPH showed potent antidiabetic actions in cells and improved glucose tolerance in mice.

AB - The antidiabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4L and Flavourzyme 500L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01-2.5 mg/ml) were tested in vitro for DPP-IV inhibition andinsulin and GLP-1 secretory activity from BRIN-BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose-dependent increase (4.2 to 5.3-fold, p<0.001) in insulin secretion from BRIN-BD11 cells and inhibited DPP-IV activity (IC50 1.18±0.04 and 1.21±0.04 mg/ml), respectively. The SGID sample produced a 1.3-fold (p<0.01) increase in GLP-1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n=8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0-120 min), p<0.05) and a consequent reduction in blood glucose concentration (p<0.01), after OGTT in mice versus controls. The BPH showed potent antidiabetic actions in cells and improved glucose tolerance in mice.

KW - boarfish protein hydrolysate

KW - insulin secretion

KW - Glucagon like peptide 1 (GLP-1)

KW - dipeptidyl peptidase IV

KW - glycaemic control

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DO - 10.1111/ijfs.13975

M3 - Article

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SP - xx

JO - International Journal of Food Science and Technology

T2 - International Journal of Food Science and Technology

JF - International Journal of Food Science and Technology

SN - 0950-5423

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M1 - xx

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