Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone

Hua Geng, Gowsihan Poologasundarampillai, Naomi Todd, Aine Devlin-Mullin, Katie Moore, Zahra Golrokhi, James Gilchrist, Eric Jones, Richard J. Potter, Chris Sutcliffe, Marie O'Briene, David Hukins, Sarah Cartmell, Christopher A. Mitchell, PETER D. LEE

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.
LanguageEnglish
Pages21169-21180
Number of pages11
JournalACS Applied Materials and Interfaces
Volume9
Issue number25
Early online date16 Jun 2017
DOIs
Publication statusPublished - 28 Jun 2017

Fingerprint

Biotransformation
Titanium
Silver
Nanoparticles
Bone and Bones
Transmission Electron Microscopy
Secondary Ion Mass Spectrometry
Osseointegration
X-Ray Microtomography
Sulfur
Osteogenesis
Joints
Infection

Keywords

  • atomic layer deposition
  • biotransformation
  • bone formation
  • osseointegration
  • silver nanoparticle coatings

Cite this

Geng, H., Poologasundarampillai, G., Todd, N., Devlin-Mullin, A., Moore, K., Golrokhi, Z., ... LEE, PETER. D. (2017). Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone. ACS Applied Materials and Interfaces, 9(25), 21169-21180. https://doi.org/10.1021/acsami.7b05150
Geng, Hua ; Poologasundarampillai, Gowsihan ; Todd, Naomi ; Devlin-Mullin, Aine ; Moore, Katie ; Golrokhi, Zahra ; Gilchrist, James ; Jones, Eric ; Potter, Richard J. ; Sutcliffe, Chris ; O'Briene, Marie ; Hukins, David ; Cartmell, Sarah ; Mitchell, Christopher A. ; LEE, PETER D. / Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone. In: ACS Applied Materials and Interfaces. 2017 ; Vol. 9, No. 25. pp. 21169-21180.
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abstract = "Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.",
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author = "Hua Geng and Gowsihan Poologasundarampillai and Naomi Todd and Aine Devlin-Mullin and Katie Moore and Zahra Golrokhi and James Gilchrist and Eric Jones and Potter, {Richard J.} and Chris Sutcliffe and Marie O'Briene and David Hukins and Sarah Cartmell and Mitchell, {Christopher A.} and LEE, {PETER D.}",
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Geng, H, Poologasundarampillai, G, Todd, N, Devlin-Mullin, A, Moore, K, Golrokhi, Z, Gilchrist, J, Jones, E, Potter, RJ, Sutcliffe, C, O'Briene, M, Hukins, D, Cartmell, S, Mitchell, CA & LEE, PETERD 2017, 'Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone', ACS Applied Materials and Interfaces, vol. 9, no. 25, pp. 21169-21180. https://doi.org/10.1021/acsami.7b05150

Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone. / Geng, Hua; Poologasundarampillai, Gowsihan; Todd, Naomi; Devlin-Mullin, Aine; Moore, Katie; Golrokhi, Zahra; Gilchrist, James; Jones, Eric; Potter, Richard J.; Sutcliffe, Chris; O'Briene, Marie; Hukins, David; Cartmell, Sarah ; Mitchell, Christopher A.; LEE, PETER D.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 25, 28.06.2017, p. 21169-21180.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biotransformation of Silver Released from Nanoparticle Coated Titanium Implants Revealed in Regenerating Bone

AU - Geng, Hua

AU - Poologasundarampillai, Gowsihan

AU - Todd, Naomi

AU - Devlin-Mullin, Aine

AU - Moore, Katie

AU - Golrokhi, Zahra

AU - Gilchrist, James

AU - Jones, Eric

AU - Potter, Richard J.

AU - Sutcliffe, Chris

AU - O'Briene, Marie

AU - Hukins, David

AU - Cartmell, Sarah

AU - Mitchell, Christopher A.

AU - LEE, PETER D.

N1 - Not compliant in UIR, but Open Access article. Evidence uploaded to 'Other files'

PY - 2017/6/28

Y1 - 2017/6/28

N2 - Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.

AB - Antimicrobial silver nanoparticle coatings have attracted interest for reducing prosthetic joint infection. However, few studies report in vivo investigations of the biotransformation of silver nanoparticles within the regenerating tissue and its impact on bone formation. We present a longitudinal investigation of the osseointegration of silver nanoparticle-coated additive manufactured titanium implants in rat tibial defects. Correlative imaging at different time points using nanoscale secondary ion mass spectrometry, transmission electron microscopy (TEM), histomorphometry, and 3D X-ray microcomputed tomography provided quantitative insight from the nano- to macroscales. The quality and quantity of newly formed bone is comparable between the uncoated and silver coated implants. The newly formed bone demonstrates a trabecular morphology with bone being located at the implant surface, and at a distance, at two weeks. Nanoscale elemental mapping of the bone–implant interface showed that silver was present primarily in the osseous tissue and colocalized with sulfur. TEM revealed silver sulfide nanoparticles in the newly regenerated bone, presenting strong evidence that the previously in vitro observed biotransformation of silver to silver sulfide occurs in vivo.

KW - atomic layer deposition

KW - biotransformation

KW - bone formation

KW - osseointegration

KW - silver nanoparticle coatings

U2 - 10.1021/acsami.7b05150

DO - 10.1021/acsami.7b05150

M3 - Article

VL - 9

SP - 21169

EP - 21180

JO - ACS Applied Materials and Interfaces

T2 - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 25

ER -