Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase

Archontoula Kalogeropoulou, Iris Plioni, Dimitra Dimitrellou, Magdalini Soupioni, Poonam Singh - Nee Nigam, Maria Kanellaki, Athanasios Koutinas

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The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cellfactory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae. The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 ◦C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 ◦C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 ◦C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield
and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads. Consequently, the results revealed a new concept for bio-ethanol
production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production.
Original languageEnglish
Article number122986
Pages (from-to)1-8
Number of pages8
Early online date30 Dec 2021
Publication statusPublished (in print/issue) - 1 Apr 2022

Bibliographical note

Funding Information:
Authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation – Food Innovation RI” (MIS 5027222), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).

Publisher Copyright:
© 2021 Elsevier Ltd


  • one-step hydrolysis and fermentation
  • genetically modified organism
  • Cell factory
  • Starch gel
  • alginate
  • polyethylene glycol
  • cellulase
  • cellobiose
  • Ethanol
  • Cell-factory
  • Hydrolysis
  • Cellobiose
  • Cellulase
  • Fuel-ethanol
  • Saccharomyces cerevisiae


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