Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase

Archontoula Kalogeropoulou; Iris Plioni; Dimitra Dimitrellou; Magdalini Soupioni; Poonam Singh - Nee Nigam; Maria Kanellaki; Athanasios Koutinas

doi:10.1016/j.fuel.2021.122986

Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase

Archontoula Kalogeropoulou, Iris Plioni, Dimitra Dimitrellou, Magdalini Soupioni, Poonam Singh - Nee Nigam, Maria Kanellaki, Athanasios Koutinas

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Abstract

The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cellfactory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae. The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 ◦C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 ◦C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 ◦C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield
and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads. Consequently, the results revealed a new concept for bio-ethanol
production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production.

Original language	English
Article number	122986
Pages (from-to)	1-8
Number of pages	8
Journal	Fuel
Volume	313
Early online date	30 Dec 2021
DOIs	https://doi.org/10.1016/j.fuel.2021.122986
Publication status	Published (in print/issue) - 1 Apr 2022

Bibliographical note

Funding Information:
Authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation – Food Innovation RI” (MIS 5027222), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

one-step hydrolysis and fermentation
genetically modified organism
Cell factory
Starch gel
alginate
polyethylene glycol
cellulase
cellobiose
Ethanol
Cell-factory
Hydrolysis
Cellobiose
Cellulase
Fuel-ethanol
Saccharomyces cerevisiae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.fuel.2021.122986

Accepted_manuscript_in_FuelAuthor Accepted Manuscript, 331 KBLicence: CC BY-NC-ND

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title = "Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase",

abstract = "The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cellfactory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae. The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 ◦C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 ◦C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 ◦C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads. Consequently, the results revealed a new concept for bio-ethanol production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production. ",

keywords = "one-step hydrolysis and fermentation, genetically modified organism, Cell factory, Starch gel, alginate, polyethylene glycol, cellulase, cellobiose, Ethanol, Cell-factory, Hydrolysis, Cellobiose, Cellulase, Fuel-ethanol, Saccharomyces cerevisiae",

author = "Archontoula Kalogeropoulou and Iris Plioni and Dimitra Dimitrellou and Magdalini Soupioni and {Singh - Nee Nigam}, Poonam and Maria Kanellaki and Athanasios Koutinas",

note = "Funding Information: Authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation – Food Innovation RI” (MIS 5027222), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund). Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

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doi = "10.1016/j.fuel.2021.122986",

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AU - Kalogeropoulou, Archontoula

AU - Plioni, Iris

AU - Dimitrellou, Dimitra

AU - Soupioni, Magdalini

AU - Singh - Nee Nigam, Poonam

AU - Kanellaki, Maria

AU - Koutinas, Athanasios

N1 - Funding Information: Authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation – Food Innovation RI” (MIS 5027222), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund). Publisher Copyright: © 2021 Elsevier Ltd

PY - 2022/4/1

Y1 - 2022/4/1

N2 - The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cellfactory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae. The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 ◦C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 ◦C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 ◦C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads. Consequently, the results revealed a new concept for bio-ethanol production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production.

AB - The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cellfactory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae. The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 ◦C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 ◦C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 ◦C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads. Consequently, the results revealed a new concept for bio-ethanol production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production.

KW - one-step hydrolysis and fermentation

KW - genetically modified organism

KW - Cell factory

KW - Starch gel

KW - alginate

KW - polyethylene glycol

KW - cellulase

KW - cellobiose

KW - Ethanol

KW - Cell-factory

KW - Hydrolysis

KW - Cellobiose

KW - Cellulase

KW - Fuel-ethanol

KW - Saccharomyces cerevisiae

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DO - 10.1016/j.fuel.2021.122986

M3 - Article

SN - 0016-2361

VL - 313

SP - 1

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JO - Fuel

JF - Fuel

M1 - 122986

ER -

Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase

Abstract

Bibliographical note

Keywords

UN SDGs

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