Biosurfactant Production by Azotobacter chroococcum Isolated from the Marine Environment

R. Thavasi, V. R. M. Subramanyam Nambaru, S. Jayalakshmi, T. Balasubramanian, Ibrahim Banat

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)


Preliminary characterization of a biosurfactant-producing Azotobacter chroococcum isolated from marine environment showed maximum biomass and biosurfactant production at 120 and 132 h, respectively, at pH 8.0, 38A degrees C, and 30aEuro degrees salinity utilizing a 2% carbon substrate. It grew and produced biosurfactant on crude oil, waste motor lubricant oil, and peanut oil cake. Peanut oil cake gave the highest biosurfactant production (4.6 mg/mL) under fermentation conditions. The biosurfactant product emulsified waste motor lubricant oil, crude oil, diesel, kerosene, naphthalene, anthracene, and xylene. Preliminary characterization of the biosurfactant using biochemical, Fourier transform infrared spectroscopy, and mass spectral analysis indicated that the biosurfactant was a lipopeptide with percentage lipid and protein proportion of 31.3:68.7.
Original languageEnglish
Pages (from-to)551-556
JournalMarine Biotechnology
Issue number5
Publication statusPublished (in print/issue) - Oct 2009


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