Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium

KSM Rahman, G Street, R Lord, G Kane, Ibrahim Banat

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages401-410
Number of pages10
Publication statusPublished - 2004
EventCOASTAL ENVIRONMENT V: INCORPORATING OIL SPILL STUDIES -
Duration: 1 Jan 2004 → …

Publication series

NameENVIRONMENTAL STUDIES SERIES

Conference

ConferenceCOASTAL ENVIRONMENT V: INCORPORATING OIL SPILL STUDIES
Period1/01/04 → …

Fingerprint

gasoline
bioremediation
hydrocarbons
biosurfactants
Vigna radiata var. radiata
chlorophyll
polluted soils
microbial growth
soil
seed germination
coir
shoots
degradation
pith
poultry manure
biodegradation
Pseudomonas aeruginosa
gardens
statistical analysis
soil sampling

Cite this

Rahman, KSM., Street, G., Lord, R., Kane, G., & Banat, I. (2004). Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. In Unknown Host Publication (pp. 401-410). (ENVIRONMENTAL STUDIES SERIES).
Rahman, KSM ; Street, G ; Lord, R ; Kane, G ; Banat, Ibrahim. / Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. Unknown Host Publication. 2004. pp. 401-410 (ENVIRONMENTAL STUDIES SERIES).
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abstract = "A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78{\%} of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1{\%} probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.",
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Rahman, KSM, Street, G, Lord, R, Kane, G & Banat, I 2004, Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. in Unknown Host Publication. ENVIRONMENTAL STUDIES SERIES, pp. 401-410, COASTAL ENVIRONMENT V: INCORPORATING OIL SPILL STUDIES, 1/01/04.

Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. / Rahman, KSM; Street, G; Lord, R; Kane, G; Banat, Ibrahim.

Unknown Host Publication. 2004. p. 401-410 (ENVIRONMENTAL STUDIES SERIES).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.

AB - A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.

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Rahman KSM, Street G, Lord R, Kane G, Banat I. Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. In Unknown Host Publication. 2004. p. 401-410. (ENVIRONMENTAL STUDIES SERIES).