Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith, and rhamnolipid biosurfactant

KSM Rahman, Ibrahim Banat, J Thahira, T Thayumanavan, P Lakshmanaperumalsamy

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The aim of the present study was to find methods for enhancing rates of hydrocarbon biodegradation in gasoline contaminated soil by ex situ bioremediation. Red soil (RS) was treated with gasoline-spilled soil (GS) from a gasoline station and different combinations of amendments were prepared using (i) mixed bacterial consortium (MC), (ii) poultry litter (PL), (iii) coir pith (CP) and (iv) rhamnolipid biosurfactant (BS) produced by Pseudomonas sp. DS10-129. The study was conducted for a period of 90 days during which bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB including seed germination, chlorophyll content, shoot and root length were measured. Approximately 67% and 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with RS+GS+MC+PL+CP+BS at 0.1% and 1%. Maximum percentage of seed germination, shoot length, root length and chlorophyll content in P. aureus were recorded after 60 days in the above amendments. Further incubation to 90 days did not exhibit significant improvements. Statistical analysis using analysis of variance (ANOVA) and Duncan's multiple range test (DMRT) revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content at a 1% probability level. All tested additives MC, PL, CP and rhamnolipid BS had significant positive effects on the bioremediation of GS. (C) 2001 Elsevier Science Ltd. All rights reserved.
LanguageEnglish
Pages25-32
JournalBioresource Technology
Volume81
Issue number1
Publication statusPublished - Jan 2002

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biosurfactants
coir
gasoline
bioremediation
pith
poultry manure
polluted soils
hydrocarbons
Vigna radiata var. radiata
seed germination
soil
chlorophyll
microbial growth
shoots
degradation
biodegradation
Pseudomonas
statistical analysis
soil sampling
analysis of variance

Cite this

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title = "Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith, and rhamnolipid biosurfactant",
abstract = "The aim of the present study was to find methods for enhancing rates of hydrocarbon biodegradation in gasoline contaminated soil by ex situ bioremediation. Red soil (RS) was treated with gasoline-spilled soil (GS) from a gasoline station and different combinations of amendments were prepared using (i) mixed bacterial consortium (MC), (ii) poultry litter (PL), (iii) coir pith (CP) and (iv) rhamnolipid biosurfactant (BS) produced by Pseudomonas sp. DS10-129. The study was conducted for a period of 90 days during which bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB including seed germination, chlorophyll content, shoot and root length were measured. Approximately 67{\%} and 78{\%} of the hydrocarbons were effectively degraded within 60 days in soil samples amended with RS+GS+MC+PL+CP+BS at 0.1{\%} and 1{\%}. Maximum percentage of seed germination, shoot length, root length and chlorophyll content in P. aureus were recorded after 60 days in the above amendments. Further incubation to 90 days did not exhibit significant improvements. Statistical analysis using analysis of variance (ANOVA) and Duncan's multiple range test (DMRT) revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content at a 1{\%} probability level. All tested additives MC, PL, CP and rhamnolipid BS had significant positive effects on the bioremediation of GS. (C) 2001 Elsevier Science Ltd. All rights reserved.",
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Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith, and rhamnolipid biosurfactant. / Rahman, KSM; Banat, Ibrahim; Thahira, J; Thayumanavan, T; Lakshmanaperumalsamy, P.

In: Bioresource Technology, Vol. 81, No. 1, 01.2002, p. 25-32.

Research output: Contribution to journalArticle

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AU - Rahman, KSM

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AU - Lakshmanaperumalsamy, P

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AB - The aim of the present study was to find methods for enhancing rates of hydrocarbon biodegradation in gasoline contaminated soil by ex situ bioremediation. Red soil (RS) was treated with gasoline-spilled soil (GS) from a gasoline station and different combinations of amendments were prepared using (i) mixed bacterial consortium (MC), (ii) poultry litter (PL), (iii) coir pith (CP) and (iv) rhamnolipid biosurfactant (BS) produced by Pseudomonas sp. DS10-129. The study was conducted for a period of 90 days during which bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB including seed germination, chlorophyll content, shoot and root length were measured. Approximately 67% and 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with RS+GS+MC+PL+CP+BS at 0.1% and 1%. Maximum percentage of seed germination, shoot length, root length and chlorophyll content in P. aureus were recorded after 60 days in the above amendments. Further incubation to 90 days did not exhibit significant improvements. Statistical analysis using analysis of variance (ANOVA) and Duncan's multiple range test (DMRT) revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content at a 1% probability level. All tested additives MC, PL, CP and rhamnolipid BS had significant positive effects on the bioremediation of GS. (C) 2001 Elsevier Science Ltd. All rights reserved.

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