Bioreactor Rhamnolipid Production Using Palm Oil Agricultural Refinery By-Products

Mohd Nazren Radzuan, James Winterburn, Ibrahim M Banat

Research output: Contribution to journalArticlepeer-review

Abstract

Palm fatty acid distillate (PFAD) and fatty acid methyl ester (FAME) are used by P. aeruginosa PAO1 to produce rhamnolipid biosurfactant. The process of fermentation producing of biosurfactant was structured in a 2 L bioreactor using 2% of PFAD and FAME as carbon sources in minimal medium and with a nitrogen concentration of 1 g L-1. Mass spectrometry results show the crude biosurfactant produced was predominantly monorhamnolipid (Rha-C10-C10) and dirhamnolipid (Rha-Rha-C10-C10) at 503 and 649 m/z value for both substrates. Maximum production of crude rhamnolipid for PFAD was 1.06 g L-1 whereas for FAME it was 2.1 g L-1, with a reduction in surface tension of Tris-HCl pH 8.0 solution to 28 mN m-1 and a critical micelle concentration (CMC) of 26 mg L-1 measured for both products. Furthermore, the 24 h emulsification indexes in kerosene, hexadecane, sunflower oil, and rapeseed oil using 1 g L-1 of crude rhamnolipid were in the range 20–50%. Consequently, PFAD and FAME, by-products from the agricultural refining of palm oil, may result in a product that has a higher added-value, rhamnolipid biosurfactant, in the process of integrated biorefinery.
Original languageEnglish
Article number2037
Number of pages15
JournalProcesses
Volume9
Issue number11
DOIs
Publication statusPublished - 14 Nov 2021

Keywords

  • biorefinery; rhamnolipid; biosurfactant; fermentation; Pseudomonas aeruginosa PAO1

Fingerprint

Dive into the research topics of 'Bioreactor Rhamnolipid Production Using Palm Oil Agricultural Refinery By-Products'. Together they form a unique fingerprint.

Cite this