Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique

P O'Hare, BJ Meenan, GA Burke, G Byrne, Denis Dowling, JA Hunt

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Hydroxyapatite (HA) is routinely used as a coating on a range of press-fit (cementless) orthopaedic implants to enhance their osseointegration. The standard plasma spraying method used to deposit a HA surface layer on such implants often contains unwanted crystal phases that can lead to coating delamination in vivo. Consequently, there has been a continuous drive to develop alternate surface modification technologies that can eliminate the problems caused by a non-optimal coating process. In this study twomethods for creating a HA layer on metal alloys that employ micro-blasting have been evaluated to determine if the inclusion of an abrasive agent can enhance the in vitro and in vivo performance of the modified surface. The first method employs direct micro-blasting using HA as the abrasive media, while the second employs a simultaneous blasting with an alumina abrasive and coincident blasting with HA as a dopant. Whereas, both methods were found to produce a surface which was enriched with HA, the respective microstructures created were significantly different. Detailed surface characterisationrevealed that the use of the abrasive produced disruption of the metal surface without producing detectable incorporation of alumina particles. Roughening of the metal surface in this way breached the passivating oxide layer and created sites which subsequently provided for impregnation, mechanical interlocking and chemical bonding of HA. The co-incident use of an alumina abrasive and a HA dopant resulted in a stable surface that demonstrated enhanced in vitro osteoblast attachment and viability as compared to the response to the surface produced using HA alone or the metal substrate control.Implantation of the surface produced by co-incident blasting with alumina and HA in a rabbit model confirmed that this surface promoted the in vivo formation of early stage lamellar bone growth.
LanguageEnglish
Pages515-522
JournalBiomaterials
Volume31
Issue number2010
DOIs
Publication statusPublished - 27 Oct 2009

Fingerprint

Durapatite
Hydroxyapatite
Blasting
Abrasives
Aluminum Oxide
Alumina
Metals
Coatings
Doping (additives)
Osseointegration
Plasma spraying
Bone Development
Osteoblasts
Orthopedics
Delamination
Impregnation
Ion implantation
Oxides
Surface treatment
Bone

Keywords

  • Hydroxyapatite coating
  • Surface treatment
  • Cell viability
  • In vivo test
  • Osseointegration
  • Bone growth

Cite this

O'Hare, P ; Meenan, BJ ; Burke, GA ; Byrne, G ; Dowling, Denis ; Hunt, JA. / Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique. In: Biomaterials. 2009 ; Vol. 31, No. 2010. pp. 515-522.
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note = "Reference text: [1] de Groot K. Degradable ceramics. In: Williams DF, editor. Biocompatibility of clinical implant materials, vol. I. Boca Rato FL: CRC Press; 1981. p. 199–222. [2] Cook SD, Thomas KA, Dalton JE, Volkman TK, Whitecloud TS, Kay JF. Hydroxylapatite coating of porous implants improves bone in-growth and interface attachment strength. J Biomed Mater Res 1992;26:989–1001. [3] Jiang HC, Rong LJ. Effect of hydroxyapatite coating on nickel release of the porous NiTi shape memory alloy fabricated by SHS method. Surf Coat Technol 2006;201:1017–21. [4] Placzek R, Ruffer M, Deuretzbacher G, Heijens E, Meiss AL. The fixation strength of hydroxyapatite-coated Schanz screws and standard stainless steel Schanz screws in lower extremity lengthening. Arch Orthop Trauma Surg 2006;126:369–73. [5] Thanner J, Kfirrholm J, Herberts R, Malchau H. Porous cups with and without hydroxylapatite–tricalcium phosphate coating: 23 matched pairs evaluated with radiostereometry. J Arthroplasty 1999;14(3):266–71. [6] Geetha M, Singh AK, Asokamani R, Gogia AK. Ti based biomaterials, the ultimate choice for orthopaedic implants – a review. Prog Mater Sci 2009;54:397–425. [7] Tanzer M, Kantor S, Rosenthall L, Bobyn JD. Femoral remodeling after porouscoated total hip arthroplasty with and without hydroxyapatite–tricalcium phosphate coating. J Arthroplasty 2001;16(5):552. [8] Mello A, Hong Z, Rossi AM, Luan L, Farina M, Querido W, et al. Osteoblast proliferation on hydroxyapatite thin coatings produced by right angle magnetron sputtering. Biomed Mater 2007;2(2):67–77. [9] Moroni A, Caja VL, Egger EL, Trinchese L, Chao EYS. Histomorphometry of hydroxyapatite coated and uncoated porous titanium bone implants. Biomaterials 1994;15(11):926. [10] Vasudev DV, Ricci JL, Sabatino C, Li P, Parsons JR. In vivo evaluation of a biomimetic apatite coating grown on titanium surfaces. J Biomed Mater Res A 2004;69A(4):629–36. [11] Le Gu´ ehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 2007;23:844–54. [12] Chang J-K, Chen C-H, Huang K-Y, Wang G-J. Eight-year results of hydroxyapatite coated hip arthroplasty. J Arthroplasty 2006;21(4):541–6. [13] Frame JW, Brady CL. The versatility of hydroxyapatite blocks in maxillofacial surgery. Br J Oral Maxillofac Surg 1987;25(6):452–64. [14] Tonino A, Rahmy A. The hydroxyapatite-ABG hip system: 5- to 7-Year results from an international multicentre study. J Arthroplasty 2000;15(3):274–82. [15] Geesink RGT, Hoefnagels NHM. Six-year results of hydroxyapatite-coated total hip replacement. J Bone Joint Surg Br 1995;77B:534–47. [16] de Groot K, Geesink R, Klein CPAT, Serekian P. Plasma sprayed coatings of hydroxylapatite. J Biomed Mater Res 1987;21:1375–81. [17] Heimann RB. Thermal spraying of biomaterials. Surf Coat Technol 2006;201:2012–9. [18] Sun L, Berndt CC, Gross KA, Kucuk A. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: a review. J Biomed Mater Res 2001;58:570–92. [19] Xue W, Tao S, Liu X, Zheng X, Ding C. In vivo evaluation of plasma sprayed hydroxyapatite coatings having different crystallinity. Biomaterials 2004;25: 415–21. [20] Wang H, Eliaz N, Xiang Z, Hsu H-P, Spector M, Hobbs LW. Early bone apposition in vivo on plasma-sprayed and electrochemically deposited hydroxyapatite coatings on titanium alloy. Biomaterials 2006;27:4192–203. [21] Ishikawa K, Miyamoto Y, Nagayama Y, Asaoka K. Blast coating method: new method of coating titanium surface with hydroxyapatite at room temperature. J Biomed Mater Res 1997;38:129–34. [22] Mano T, Ueyama Y, Ishikawa K, Matsumura T, Suzuki K. Initial tissue response to a titanium implant coated with apatite at room temperature using a blast coating method. Biomaterials 2002;23:1931–6. [23] Nakada H, Sakae T, LeGeros RZ, LeGeros JP, Suwa T, Numata Y, et al. Early tissue response to modified implant surfaces using back scattered imaging. Implant Dent 2007;16(3):281–9. [24] Yang S, Man HC, Xing W, Zheng X. Adhesion strength of plasma-sprayed hydroxyapatite coatings on laser gas-nitrided pure titanium. Surf Coat Technol 2009;203(20–21):3116–22. [25] Gbureck U, Masten A, Probst J, Thull R. Tribochemical structuring and coating of implant metal surfaces with titanium oxide and hydroxyapatite layers. Mater Sci Eng C 2003;23:461–5. [26] O’Donoghue J, Haverty D, Method of doping surfaces, PCT application No. WO2008/033867, 2008. [27] Kueng W, Silber E, Eppenberger U. Quantification of cells cultured on 96-well plates. Anal Biochem 1989;182(1):16–9. [28] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55–63. [29] Burghardt RC, Barhoumi R, Lewis EH, Hartford Bailey R, Pyle KA, Clement BA, et al. Patulin-induced cellular toxicity: a vital fluorescence study. Toxicol Appl Pharmacol 1992;112(2):235–44",
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Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique. / O'Hare, P; Meenan, BJ; Burke, GA; Byrne, G; Dowling, Denis; Hunt, JA.

In: Biomaterials, Vol. 31, No. 2010, 27.10.2009, p. 515-522.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique

AU - O'Hare, P

AU - Meenan, BJ

AU - Burke, GA

AU - Byrne, G

AU - Dowling, Denis

AU - Hunt, JA

N1 - Reference text: [1] de Groot K. Degradable ceramics. In: Williams DF, editor. Biocompatibility of clinical implant materials, vol. I. Boca Rato FL: CRC Press; 1981. p. 199–222. [2] Cook SD, Thomas KA, Dalton JE, Volkman TK, Whitecloud TS, Kay JF. Hydroxylapatite coating of porous implants improves bone in-growth and interface attachment strength. J Biomed Mater Res 1992;26:989–1001. [3] Jiang HC, Rong LJ. Effect of hydroxyapatite coating on nickel release of the porous NiTi shape memory alloy fabricated by SHS method. Surf Coat Technol 2006;201:1017–21. [4] Placzek R, Ruffer M, Deuretzbacher G, Heijens E, Meiss AL. The fixation strength of hydroxyapatite-coated Schanz screws and standard stainless steel Schanz screws in lower extremity lengthening. Arch Orthop Trauma Surg 2006;126:369–73. [5] Thanner J, Kfirrholm J, Herberts R, Malchau H. Porous cups with and without hydroxylapatite–tricalcium phosphate coating: 23 matched pairs evaluated with radiostereometry. J Arthroplasty 1999;14(3):266–71. [6] Geetha M, Singh AK, Asokamani R, Gogia AK. Ti based biomaterials, the ultimate choice for orthopaedic implants – a review. Prog Mater Sci 2009;54:397–425. [7] Tanzer M, Kantor S, Rosenthall L, Bobyn JD. Femoral remodeling after porouscoated total hip arthroplasty with and without hydroxyapatite–tricalcium phosphate coating. J Arthroplasty 2001;16(5):552. [8] Mello A, Hong Z, Rossi AM, Luan L, Farina M, Querido W, et al. Osteoblast proliferation on hydroxyapatite thin coatings produced by right angle magnetron sputtering. Biomed Mater 2007;2(2):67–77. [9] Moroni A, Caja VL, Egger EL, Trinchese L, Chao EYS. Histomorphometry of hydroxyapatite coated and uncoated porous titanium bone implants. Biomaterials 1994;15(11):926. [10] Vasudev DV, Ricci JL, Sabatino C, Li P, Parsons JR. In vivo evaluation of a biomimetic apatite coating grown on titanium surfaces. J Biomed Mater Res A 2004;69A(4):629–36. [11] Le Gu´ ehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 2007;23:844–54. [12] Chang J-K, Chen C-H, Huang K-Y, Wang G-J. Eight-year results of hydroxyapatite coated hip arthroplasty. J Arthroplasty 2006;21(4):541–6. [13] Frame JW, Brady CL. The versatility of hydroxyapatite blocks in maxillofacial surgery. Br J Oral Maxillofac Surg 1987;25(6):452–64. [14] Tonino A, Rahmy A. The hydroxyapatite-ABG hip system: 5- to 7-Year results from an international multicentre study. J Arthroplasty 2000;15(3):274–82. [15] Geesink RGT, Hoefnagels NHM. Six-year results of hydroxyapatite-coated total hip replacement. J Bone Joint Surg Br 1995;77B:534–47. [16] de Groot K, Geesink R, Klein CPAT, Serekian P. Plasma sprayed coatings of hydroxylapatite. J Biomed Mater Res 1987;21:1375–81. [17] Heimann RB. Thermal spraying of biomaterials. Surf Coat Technol 2006;201:2012–9. [18] Sun L, Berndt CC, Gross KA, Kucuk A. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: a review. J Biomed Mater Res 2001;58:570–92. [19] Xue W, Tao S, Liu X, Zheng X, Ding C. In vivo evaluation of plasma sprayed hydroxyapatite coatings having different crystallinity. Biomaterials 2004;25: 415–21. [20] Wang H, Eliaz N, Xiang Z, Hsu H-P, Spector M, Hobbs LW. Early bone apposition in vivo on plasma-sprayed and electrochemically deposited hydroxyapatite coatings on titanium alloy. Biomaterials 2006;27:4192–203. [21] Ishikawa K, Miyamoto Y, Nagayama Y, Asaoka K. Blast coating method: new method of coating titanium surface with hydroxyapatite at room temperature. J Biomed Mater Res 1997;38:129–34. [22] Mano T, Ueyama Y, Ishikawa K, Matsumura T, Suzuki K. Initial tissue response to a titanium implant coated with apatite at room temperature using a blast coating method. Biomaterials 2002;23:1931–6. [23] Nakada H, Sakae T, LeGeros RZ, LeGeros JP, Suwa T, Numata Y, et al. Early tissue response to modified implant surfaces using back scattered imaging. Implant Dent 2007;16(3):281–9. [24] Yang S, Man HC, Xing W, Zheng X. Adhesion strength of plasma-sprayed hydroxyapatite coatings on laser gas-nitrided pure titanium. Surf Coat Technol 2009;203(20–21):3116–22. [25] Gbureck U, Masten A, Probst J, Thull R. Tribochemical structuring and coating of implant metal surfaces with titanium oxide and hydroxyapatite layers. Mater Sci Eng C 2003;23:461–5. [26] O’Donoghue J, Haverty D, Method of doping surfaces, PCT application No. WO2008/033867, 2008. [27] Kueng W, Silber E, Eppenberger U. Quantification of cells cultured on 96-well plates. Anal Biochem 1989;182(1):16–9. [28] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55–63. [29] Burghardt RC, Barhoumi R, Lewis EH, Hartford Bailey R, Pyle KA, Clement BA, et al. Patulin-induced cellular toxicity: a vital fluorescence study. Toxicol Appl Pharmacol 1992;112(2):235–44

PY - 2009/10/27

Y1 - 2009/10/27

N2 - Hydroxyapatite (HA) is routinely used as a coating on a range of press-fit (cementless) orthopaedic implants to enhance their osseointegration. The standard plasma spraying method used to deposit a HA surface layer on such implants often contains unwanted crystal phases that can lead to coating delamination in vivo. Consequently, there has been a continuous drive to develop alternate surface modification technologies that can eliminate the problems caused by a non-optimal coating process. In this study twomethods for creating a HA layer on metal alloys that employ micro-blasting have been evaluated to determine if the inclusion of an abrasive agent can enhance the in vitro and in vivo performance of the modified surface. The first method employs direct micro-blasting using HA as the abrasive media, while the second employs a simultaneous blasting with an alumina abrasive and coincident blasting with HA as a dopant. Whereas, both methods were found to produce a surface which was enriched with HA, the respective microstructures created were significantly different. Detailed surface characterisationrevealed that the use of the abrasive produced disruption of the metal surface without producing detectable incorporation of alumina particles. Roughening of the metal surface in this way breached the passivating oxide layer and created sites which subsequently provided for impregnation, mechanical interlocking and chemical bonding of HA. The co-incident use of an alumina abrasive and a HA dopant resulted in a stable surface that demonstrated enhanced in vitro osteoblast attachment and viability as compared to the response to the surface produced using HA alone or the metal substrate control.Implantation of the surface produced by co-incident blasting with alumina and HA in a rabbit model confirmed that this surface promoted the in vivo formation of early stage lamellar bone growth.

AB - Hydroxyapatite (HA) is routinely used as a coating on a range of press-fit (cementless) orthopaedic implants to enhance their osseointegration. The standard plasma spraying method used to deposit a HA surface layer on such implants often contains unwanted crystal phases that can lead to coating delamination in vivo. Consequently, there has been a continuous drive to develop alternate surface modification technologies that can eliminate the problems caused by a non-optimal coating process. In this study twomethods for creating a HA layer on metal alloys that employ micro-blasting have been evaluated to determine if the inclusion of an abrasive agent can enhance the in vitro and in vivo performance of the modified surface. The first method employs direct micro-blasting using HA as the abrasive media, while the second employs a simultaneous blasting with an alumina abrasive and coincident blasting with HA as a dopant. Whereas, both methods were found to produce a surface which was enriched with HA, the respective microstructures created were significantly different. Detailed surface characterisationrevealed that the use of the abrasive produced disruption of the metal surface without producing detectable incorporation of alumina particles. Roughening of the metal surface in this way breached the passivating oxide layer and created sites which subsequently provided for impregnation, mechanical interlocking and chemical bonding of HA. The co-incident use of an alumina abrasive and a HA dopant resulted in a stable surface that demonstrated enhanced in vitro osteoblast attachment and viability as compared to the response to the surface produced using HA alone or the metal substrate control.Implantation of the surface produced by co-incident blasting with alumina and HA in a rabbit model confirmed that this surface promoted the in vivo formation of early stage lamellar bone growth.

KW - Hydroxyapatite coating

KW - Surface treatment

KW - Cell viability

KW - In vivo test

KW - Osseointegration

KW - Bone growth

U2 - 10.1016/j.biomaterials.2009.09.067

DO - 10.1016/j.biomaterials.2009.09.067

M3 - Article

VL - 31

SP - 515

EP - 522

JO - Biomaterials

T2 - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 2010

ER -