Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery

S. M. M. Dastgheib, M. A. Amoozegar, E. Elahi, S. Asad, Ibrahim Banat

Research output: Contribution to journalArticle

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Abstract

A halothermotolerant Gram-positive spore-forming bacterium was isolated from petroleum reservoirs in Iran and identified as Bacillus licheniformis sp. strain ACO1 by phenotypic characterization and 16S rRNA analysis. It showed a high capacity for bioemulsifier production and grew up to 60 degrees C with NaCl at 180 g 1(-1). The optimum NaCl concentration, pH and temperature for bioemulsifier production were 4% (w/v), 8.0, and 45 degrees C, respectively. Although ACO1 did not utilize hydrocarbons, it had a high emulsifying activity (E (24) = 65 +/- 5%) on different hydrophobic substrates. Emulsification was optimal while growing on yeast extract as the sole carbon source and NaNO3 as the nitrogen source. The efficiency of the residual oil recovery increased by 22% after in situ growth of B. licheniformis ACO1 in a sand-pack model saturated with liquid paraffin.
LanguageEnglish
Pages263-270
JournalBiotechnology Letters
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Bacillus licheniformis
emulsifiers
Bacillus (bacteria)
emulsifying
spore-forming bacteria
oils
yeast extract
Gram-positive bacteria
petroleum
alkanes
hydrocarbons
Iran
ribosomal RNA
sand
liquids
carbon
nitrogen
temperature

Cite this

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title = "Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery",
abstract = "A halothermotolerant Gram-positive spore-forming bacterium was isolated from petroleum reservoirs in Iran and identified as Bacillus licheniformis sp. strain ACO1 by phenotypic characterization and 16S rRNA analysis. It showed a high capacity for bioemulsifier production and grew up to 60 degrees C with NaCl at 180 g 1(-1). The optimum NaCl concentration, pH and temperature for bioemulsifier production were 4{\%} (w/v), 8.0, and 45 degrees C, respectively. Although ACO1 did not utilize hydrocarbons, it had a high emulsifying activity (E (24) = 65 +/- 5{\%}) on different hydrophobic substrates. Emulsification was optimal while growing on yeast extract as the sole carbon source and NaNO3 as the nitrogen source. The efficiency of the residual oil recovery increased by 22{\%} after in situ growth of B. licheniformis ACO1 in a sand-pack model saturated with liquid paraffin.",
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Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. / Dastgheib, S. M. M.; Amoozegar, M. A.; Elahi, E.; Asad, S.; Banat, Ibrahim.

In: Biotechnology Letters, Vol. 30, No. 2, 02.2008, p. 263-270.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Dastgheib, S. M. M.

AU - Amoozegar, M. A.

AU - Elahi, E.

AU - Asad, S.

AU - Banat, Ibrahim

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AB - A halothermotolerant Gram-positive spore-forming bacterium was isolated from petroleum reservoirs in Iran and identified as Bacillus licheniformis sp. strain ACO1 by phenotypic characterization and 16S rRNA analysis. It showed a high capacity for bioemulsifier production and grew up to 60 degrees C with NaCl at 180 g 1(-1). The optimum NaCl concentration, pH and temperature for bioemulsifier production were 4% (w/v), 8.0, and 45 degrees C, respectively. Although ACO1 did not utilize hydrocarbons, it had a high emulsifying activity (E (24) = 65 +/- 5%) on different hydrophobic substrates. Emulsification was optimal while growing on yeast extract as the sole carbon source and NaNO3 as the nitrogen source. The efficiency of the residual oil recovery increased by 22% after in situ growth of B. licheniformis ACO1 in a sand-pack model saturated with liquid paraffin.

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