Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21

G Verma, Poonam Singh - Nee Nigam, D Singh, K Chaudhary

Research output: Contribution to journalArticle

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Abstract

Ethanol production by a coculture of Saccharomyces diastaticus and Saccharomyces cerevisiae 21 was 24.8 g/l using raw unhydrolysed starch in a single-step fermentation. This was 48% higher than the yield obtained with the monoculture of S. diastaticus (16.8 g/l). The maximum ethanol fermentation efficiency was achieved (93% of the theoretical value) using 60 g/l starch concentration. In another coculture fermentation with E. capsularis and S. cerevisiae 21, maximum ethanol yield was 16.0 g/l, higher than the yield with the monoculture of Endomycopsis capsularis. In batch fermentations using cocultures maximum ethanol production occurred in 48 h of fermentation at 30 degrees C using 60 g/l starch. Fermentation efficiency was found lower in a two-step process using sr-amylase and glucoamylase-treated starch. (C) 1999 Elsevier Science Ltd. All rights reserved.
LanguageEnglish
Pages261-266
JournalBioresource Technology
Volume72
Issue number3
Publication statusPublished - May 2000

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coculture
biotransformation
Saccharomyces cerevisiae
ethanol
fermentation
starch
yeasts
ethanol production
ethanol fermentation
glucan 1,4-alpha-glucosidase
batch fermentation
Saccharomyces
amylases

Cite this

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title = "Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21",
abstract = "Ethanol production by a coculture of Saccharomyces diastaticus and Saccharomyces cerevisiae 21 was 24.8 g/l using raw unhydrolysed starch in a single-step fermentation. This was 48{\%} higher than the yield obtained with the monoculture of S. diastaticus (16.8 g/l). The maximum ethanol fermentation efficiency was achieved (93{\%} of the theoretical value) using 60 g/l starch concentration. In another coculture fermentation with E. capsularis and S. cerevisiae 21, maximum ethanol yield was 16.0 g/l, higher than the yield with the monoculture of Endomycopsis capsularis. In batch fermentations using cocultures maximum ethanol production occurred in 48 h of fermentation at 30 degrees C using 60 g/l starch. Fermentation efficiency was found lower in a two-step process using sr-amylase and glucoamylase-treated starch. (C) 1999 Elsevier Science Ltd. All rights reserved.",
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Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21. / Verma, G; Singh - Nee Nigam, Poonam; Singh, D; Chaudhary, K.

Vol. 72, No. 3, 05.2000, p. 261-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21

AU - Verma, G

AU - Singh - Nee Nigam, Poonam

AU - Singh, D

AU - Chaudhary, K

PY - 2000/5

Y1 - 2000/5

N2 - Ethanol production by a coculture of Saccharomyces diastaticus and Saccharomyces cerevisiae 21 was 24.8 g/l using raw unhydrolysed starch in a single-step fermentation. This was 48% higher than the yield obtained with the monoculture of S. diastaticus (16.8 g/l). The maximum ethanol fermentation efficiency was achieved (93% of the theoretical value) using 60 g/l starch concentration. In another coculture fermentation with E. capsularis and S. cerevisiae 21, maximum ethanol yield was 16.0 g/l, higher than the yield with the monoculture of Endomycopsis capsularis. In batch fermentations using cocultures maximum ethanol production occurred in 48 h of fermentation at 30 degrees C using 60 g/l starch. Fermentation efficiency was found lower in a two-step process using sr-amylase and glucoamylase-treated starch. (C) 1999 Elsevier Science Ltd. All rights reserved.

AB - Ethanol production by a coculture of Saccharomyces diastaticus and Saccharomyces cerevisiae 21 was 24.8 g/l using raw unhydrolysed starch in a single-step fermentation. This was 48% higher than the yield obtained with the monoculture of S. diastaticus (16.8 g/l). The maximum ethanol fermentation efficiency was achieved (93% of the theoretical value) using 60 g/l starch concentration. In another coculture fermentation with E. capsularis and S. cerevisiae 21, maximum ethanol yield was 16.0 g/l, higher than the yield with the monoculture of Endomycopsis capsularis. In batch fermentations using cocultures maximum ethanol production occurred in 48 h of fermentation at 30 degrees C using 60 g/l starch. Fermentation efficiency was found lower in a two-step process using sr-amylase and glucoamylase-treated starch. (C) 1999 Elsevier Science Ltd. All rights reserved.

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