Biocontrol of Burkholderia cepacia complex bacteria and bacterial phytopathogens by Bdellovibrio bacteriovorus.

Damien McNeeley, R.M. Chanyi, J Dooley, John E. Moore, Susan F. Koval

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing. The prey range of these isolates was tested on Burkholderia cepacia complex bacteria and several phytopathogenic bacteria of agricultural importance. Of 31 strains of the Burkholderia cepacia complex tested, only 4 were resistant to predation by strain DM7C. A subset of 9 of the prey tested were also susceptible to strains DM8A and DM11A. Of 12 phytopathogens tested, 4 were resistant to strains DM7C and DM8A, and only 2 were resistant to strain DM11A. Thus,Bdellovibrio bacteriovorus strains retrieved from environmental samples on 2 Burkholderia cenocepacia isolates from cystic fibrosis patients did not distinguish in their prey range between other isolates of that pathogen or phytopathogens. Such strains hold promise as potential wide-spectrum biocontrol agents.
LanguageEnglish
Pages350-358
Number of pages9
JournalCanadian Journal of Microbiology
Volume63
Issue number4
Early online date22 Dec 2016
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Bdellovibrio bacteriovorus
Burkholderia cepacia complex
plant pathogens
biological control
bacteria
Bdellovibrio
predators
plant pathogenic bacteria
enrichment culture
cystic fibrosis
sewage
Gram-negative bacteria
biological control agents
life cycle (organisms)
ribosomal RNA
predation

Keywords

  • Bdellovibrio
  • Burkholderia cepacia complex
  • bacteria,
  • phytopathogen
  • predator
  • biocontrol

Cite this

McNeeley, Damien ; Chanyi, R.M. ; Dooley, J ; Moore, John E. ; Koval, Susan F. . / Biocontrol of Burkholderia cepacia complex bacteria and bacterial phytopathogens by Bdellovibrio bacteriovorus. 2017 ; Vol. 63, No. 4. pp. 350-358.
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Biocontrol of Burkholderia cepacia complex bacteria and bacterial phytopathogens by Bdellovibrio bacteriovorus. / McNeeley, Damien; Chanyi, R.M.; Dooley, J; Moore, John E. ; Koval, Susan F. .

Vol. 63, No. 4, 01.04.2017, p. 350-358.

Research output: Contribution to journalArticle

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