We demonstrate a novel and highly sensitive electrochemical detection of estrone based on an immunosensor platform, composed of bioassembled nanocircuits of Mo6S9-xIx nanowires (MoSI NWs) covalently connected to anti-estrone antibodies. The one-step, label-free, and quantitative detection of estrone is realized by employing the [Ru(NH3)(6)](3+/2+) redox ions to sense anti-estrone antibody and estrone interactions. The MoSI NWs/anti-estrone antibody nanocircuit architectures provide an amplification and conductive pathway for the specific electrochemical sensing of estrone hapten. A detection limit of 1.4 pg.mL(-1) was achieved in contrast to previous electrochemical techniques in which the sensitivity was limited to the nanomolar range.
Sun, N., McMullan, M., Papakonstantinou, P., Gao, H., Zhang, XX., Mihailovic, D., & Li, MX. (2008). Bioassembled nanocircuits of Mo6S9-xIx nanowires for electrochemical immunodetection of estrone hapten. Analytical Chemistry, 80(10), 3593-3597. https://doi.org/10.1021/ac7024893