Binding sites for tachykinin peptides in the brain and stomach of the dogfish, Scyliorhinus canicula

Paul L.M. Van Giersbergen; J. Michael Conlon; Stephen H. Buck

doi:10.1016/0196-9781(91)90075-Z

Binding sites for tachykinin peptides in the brain and stomach of the dogfish, Scyliorhinus canicula

Paul L.M. Van Giersbergen
, J. Michael Conlon
, Stephen H. Buck

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

In membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. One site specifically bound [¹²⁵I]-Bolton-Hunter substance P (BH-SP) and the rank potency of tachykinins to compete for BH-SP binding revealed similarities with the rank potency of an NK₁ receptor. The pharmacology of the other site, which specifically bound [¹²⁵I]-Bolton-Hunter scyliorhinin II (BH-Scy II), did not resemble any of the mammalian tachykinin receptors. The rank potency to inhibit BH-Scy II binding to this second site was: scyliorhinin II ≈ scyliorhinin I > eledoisin ≈ substance P ≈ neurokinin A > phyllomedusin ≈ physalaemin > [Sar⁹Met(O₂)¹¹]substance P. Neurokinin B and senktide did not displace BH-Scy II binding. In addition, nucleotide analogues inhibited BH-SP binding but not BH-Scy II binding. Our binding data suggest the existence of a mammalian-like NK₁ receptor and of a nonmammalian tachykinin receptor in the dogfish.

Original language	English
Pages (from-to)	1161-1163
Number of pages	3
Journal	Peptides
Volume	12
Issue number	5
DOIs	https://doi.org/10.1016/0196-9781(91)90075-Z
Publication status	Published (in print/issue) - 1991

Funding

The authors gratefully acknowledge Christian J. Mussap for assistance with preparation of BH-Scy H and the National Health and Medical Research Council of Australia for partial support of this project.

Keywords

Brain
Dogfish
G-protein
Scyliorhinus caniculus
Stomach
Tachykinin receptors
Tachykinins
[I]-Bolton-Hunter scyliorhinin II
[I]-Bolton-Hunter substance P

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@article{474fa7d2e52f4a399678d488a0639fcd,

title = "Binding sites for tachykinin peptides in the brain and stomach of the dogfish, Scyliorhinus canicula",

abstract = "In membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. One site specifically bound [125I]-Bolton-Hunter substance P (BH-SP) and the rank potency of tachykinins to compete for BH-SP binding revealed similarities with the rank potency of an NK1 receptor. The pharmacology of the other site, which specifically bound [125I]-Bolton-Hunter scyliorhinin II (BH-Scy II), did not resemble any of the mammalian tachykinin receptors. The rank potency to inhibit BH-Scy II binding to this second site was: scyliorhinin II ≈ scyliorhinin I > eledoisin ≈ substance P ≈ neurokinin A > phyllomedusin ≈ physalaemin > [Sar9Met(O2)11]substance P. Neurokinin B and senktide did not displace BH-Scy II binding. In addition, nucleotide analogues inhibited BH-SP binding but not BH-Scy II binding. Our binding data suggest the existence of a mammalian-like NK1 receptor and of a nonmammalian tachykinin receptor in the dogfish.",

keywords = "Brain, Dogfish, G-protein, Scyliorhinus caniculus, Stomach, Tachykinin receptors, Tachykinins, [I]-Bolton-Hunter scyliorhinin II, [I]-Bolton-Hunter substance P",

author = "\{Van Giersbergen\}, \{Paul L.M.\} and Conlon, \{J. Michael\} and Buck, \{Stephen H.\}",

year = "1991",

doi = "10.1016/0196-9781(91)90075-Z",

language = "English",

volume = "12",

pages = "1161--1163",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - Binding sites for tachykinin peptides in the brain and stomach of the dogfish, Scyliorhinus canicula

AU - Van Giersbergen, Paul L.M.

AU - Conlon, J. Michael

AU - Buck, Stephen H.

PY - 1991

Y1 - 1991

N2 - In membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. One site specifically bound [125I]-Bolton-Hunter substance P (BH-SP) and the rank potency of tachykinins to compete for BH-SP binding revealed similarities with the rank potency of an NK1 receptor. The pharmacology of the other site, which specifically bound [125I]-Bolton-Hunter scyliorhinin II (BH-Scy II), did not resemble any of the mammalian tachykinin receptors. The rank potency to inhibit BH-Scy II binding to this second site was: scyliorhinin II ≈ scyliorhinin I > eledoisin ≈ substance P ≈ neurokinin A > phyllomedusin ≈ physalaemin > [Sar9Met(O2)11]substance P. Neurokinin B and senktide did not displace BH-Scy II binding. In addition, nucleotide analogues inhibited BH-SP binding but not BH-Scy II binding. Our binding data suggest the existence of a mammalian-like NK1 receptor and of a nonmammalian tachykinin receptor in the dogfish.

AB - In membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. One site specifically bound [125I]-Bolton-Hunter substance P (BH-SP) and the rank potency of tachykinins to compete for BH-SP binding revealed similarities with the rank potency of an NK1 receptor. The pharmacology of the other site, which specifically bound [125I]-Bolton-Hunter scyliorhinin II (BH-Scy II), did not resemble any of the mammalian tachykinin receptors. The rank potency to inhibit BH-Scy II binding to this second site was: scyliorhinin II ≈ scyliorhinin I > eledoisin ≈ substance P ≈ neurokinin A > phyllomedusin ≈ physalaemin > [Sar9Met(O2)11]substance P. Neurokinin B and senktide did not displace BH-Scy II binding. In addition, nucleotide analogues inhibited BH-SP binding but not BH-Scy II binding. Our binding data suggest the existence of a mammalian-like NK1 receptor and of a nonmammalian tachykinin receptor in the dogfish.

KW - Brain

KW - Dogfish

KW - G-protein

KW - Scyliorhinus caniculus

KW - Stomach

KW - Tachykinin receptors

KW - Tachykinins

KW - [I]-Bolton-Hunter scyliorhinin II

KW - [I]-Bolton-Hunter substance P

UR - https://www.scopus.com/pages/publications/0025990935

U2 - 10.1016/0196-9781(91)90075-Z

DO - 10.1016/0196-9781(91)90075-Z

M3 - Article

C2 - 1666186

AN - SCOPUS:0025990935

SN - 0196-9781

VL - 12

SP - 1161

EP - 1163

JO - Peptides

JF - Peptides

IS - 5

ER -

Binding sites for tachykinin peptides in the brain and stomach of the dogfish, Scyliorhinus canicula

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