TY - JOUR
T1 - Binding sites for peptide-histidine-isoleucine (PHI) on rat insulinoma-derived RINm5F cells
AU - Göke, Rüdiger
AU - Conlon, J. Michael
PY - 1988/12
Y1 - 1988/12
N2 - Specific binding sites for 125I-labelled rat peptide-histidine-isoleucine (PHI) were identified on rat insulinoma-derived RINm5F cells. The concentrations of peptides producing half-maximal displacement of label were rat PHI, 0.36 ± 0.14 nM, vasoactive intestinal polypeptide (VIP), 0.38 ± 0.13 nM and secretin, approximately 0.2 μM. Glucagon and glucagon-like peptide-1(7-36)amide were without effect on binding. PHI and VIP produced dose-dependent increases in cAMP production in the cells that were significantly (P < 0.05) above unstimulated rates for ligand concentrations between 10-8 and 10 -6 M. Both PHI and VIP produced a small but significant (P < 0.05) enhancement in the rate of release of immunoreactive insulin from the cells but the effect was not dose dependent.
AB - Specific binding sites for 125I-labelled rat peptide-histidine-isoleucine (PHI) were identified on rat insulinoma-derived RINm5F cells. The concentrations of peptides producing half-maximal displacement of label were rat PHI, 0.36 ± 0.14 nM, vasoactive intestinal polypeptide (VIP), 0.38 ± 0.13 nM and secretin, approximately 0.2 μM. Glucagon and glucagon-like peptide-1(7-36)amide were without effect on binding. PHI and VIP produced dose-dependent increases in cAMP production in the cells that were significantly (P < 0.05) above unstimulated rates for ligand concentrations between 10-8 and 10 -6 M. Both PHI and VIP produced a small but significant (P < 0.05) enhancement in the rate of release of immunoreactive insulin from the cells but the effect was not dose dependent.
KW - Cyclic AMP
KW - Insulin release
KW - Vasoactive intestinal polypeptide
UR - http://www.scopus.com/inward/record.url?scp=0024226728&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(88)90180-3
DO - 10.1016/0303-7207(88)90180-3
M3 - Article
C2 - 2850958
AN - SCOPUS:0024226728
SN - 0303-7207
VL - 60
SP - 211
EP - 215
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 2-3
ER -