Behavioural evaluation of mouse models of type 2 diabetes

Barry Hitchen; Kelly Norwood; Victor A Gault; J.C. Leslie

doi:10.1016/j.lmot.2021.101730

Behavioural evaluation of mouse models of type 2 diabetes

Barry Hitchen, Kelly Norwood, Victor A Gault, J.C. Leslie

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Abstract

Motivation for food reinforcers in mouse models of type 2 diabetes was examined in three experiments. In all experiments, physiological measures indicated the presence of type 2 diabetes. In Experiment 1, high-fat fed Swiss TO mice and lean controls showed a preference for high-fat corn oil reinforcers over high-sugar syrup reinforcers in a T-Maze task, but there were no differences between the two groups. In Experiment 2, high-fat fed Swiss TO mice and lean controls responded for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets, and satiation or extinction reduced break points, there were no differences between the groups. In Experiment 3, streptozotocin treatment was used to induce type 2 diabetes in C57Bl/6j mice that were compared with controls while responding for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets before and after streptozotocin treatment, and satiation or extinction reduced break points, there were no differences between the groups. Parameters for a more complex method of assessment of progressive ratio behaviour derived from Killeen’s Mathematical Principles of Reinforcement were also computed. While the parameter associated with incentive value was higher for corn oil than for food pellets, parameters were not significantly affected by streptozotocin treatment. Overall, a range of behavioural measures of food motivation failed to reveal effects of changes relative to controls in mice that showed physiological evidence of type 2 diabetes.

Original language	English
Article number	101730
Number of pages	12
Journal	Learning and Motivation
Volume	74
Early online date	6 May 2021
DOIs	https://doi.org/10.1016/j.lmot.2021.101730
Publication status	Published (in print/issue) - 31 May 2021

Bibliographical note

This paper is a respectful tribute to the late Jim Wright who is remembered both for his personal kindness and integrity and his unfailing commitment to the experimental analysis of behaviour. The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland. We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3.

Funding Information:
The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland . We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3.

Publisher Copyright:
© 2021 The Author(s)

Keywords

Type 2 diabetes
mouse models of diabetes
T-maze
progressive ratio schedule
high-fat diet
streptozotocin treatment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Hitchen et al(2021)1-s2.0-S0023969021000242-mainFinal published version, 1.32 MBLicence: CC BY

Cite this

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title = "Behavioural evaluation of mouse models of type 2 diabetes",

abstract = "Motivation for food reinforcers in mouse models of type 2 diabetes was examined in three experiments. In all experiments, physiological measures indicated the presence of type 2 diabetes. In Experiment 1, high-fat fed Swiss TO mice and lean controls showed a preference for high-fat corn oil reinforcers over high-sugar syrup reinforcers in a T-Maze task, but there were no differences between the two groups. In Experiment 2, high-fat fed Swiss TO mice and lean controls responded for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets, and satiation or extinction reduced break points, there were no differences between the groups. In Experiment 3, streptozotocin treatment was used to induce type 2 diabetes in C57Bl/6j mice that were compared with controls while responding for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets before and after streptozotocin treatment, and satiation or extinction reduced break points, there were no differences between the groups. Parameters for a more complex method of assessment of progressive ratio behaviour derived from Killeen{\textquoteright}s Mathematical Principles of Reinforcement were also computed. While the parameter associated with incentive value was higher for corn oil than for food pellets, parameters were not significantly affected by streptozotocin treatment. Overall, a range of behavioural measures of food motivation failed to reveal effects of changes relative to controls in mice that showed physiological evidence of type 2 diabetes.",

keywords = "Type 2 diabetes, mouse models of diabetes, T-maze, progressive ratio schedule, high-fat diet, streptozotocin treatment",

author = "Barry Hitchen and Kelly Norwood and Gault, {Victor A} and J.C. Leslie",

note = "This paper is a respectful tribute to the late Jim Wright who is remembered both for his personal kindness and integrity and his unfailing commitment to the experimental analysis of behaviour. The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland. We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3. Funding Information: The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland . We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

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doi = "10.1016/j.lmot.2021.101730",

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N1 - This paper is a respectful tribute to the late Jim Wright who is remembered both for his personal kindness and integrity and his unfailing commitment to the experimental analysis of behaviour. The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland. We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3. Funding Information: The first author was supported by a PhD studentship from the Department for Employment and Learning Northern Ireland . We are indebted to Peter Killeen for providing the Solver program in Microsoft Excel to derive parameter estimates in Experiment 3. Publisher Copyright: © 2021 The Author(s)

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Y1 - 2021/5/31

N2 - Motivation for food reinforcers in mouse models of type 2 diabetes was examined in three experiments. In all experiments, physiological measures indicated the presence of type 2 diabetes. In Experiment 1, high-fat fed Swiss TO mice and lean controls showed a preference for high-fat corn oil reinforcers over high-sugar syrup reinforcers in a T-Maze task, but there were no differences between the two groups. In Experiment 2, high-fat fed Swiss TO mice and lean controls responded for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets, and satiation or extinction reduced break points, there were no differences between the groups. In Experiment 3, streptozotocin treatment was used to induce type 2 diabetes in C57Bl/6j mice that were compared with controls while responding for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets before and after streptozotocin treatment, and satiation or extinction reduced break points, there were no differences between the groups. Parameters for a more complex method of assessment of progressive ratio behaviour derived from Killeen’s Mathematical Principles of Reinforcement were also computed. While the parameter associated with incentive value was higher for corn oil than for food pellets, parameters were not significantly affected by streptozotocin treatment. Overall, a range of behavioural measures of food motivation failed to reveal effects of changes relative to controls in mice that showed physiological evidence of type 2 diabetes.

AB - Motivation for food reinforcers in mouse models of type 2 diabetes was examined in three experiments. In all experiments, physiological measures indicated the presence of type 2 diabetes. In Experiment 1, high-fat fed Swiss TO mice and lean controls showed a preference for high-fat corn oil reinforcers over high-sugar syrup reinforcers in a T-Maze task, but there were no differences between the two groups. In Experiment 2, high-fat fed Swiss TO mice and lean controls responded for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets, and satiation or extinction reduced break points, there were no differences between the groups. In Experiment 3, streptozotocin treatment was used to induce type 2 diabetes in C57Bl/6j mice that were compared with controls while responding for corn oil or food pellet reinforcers on progressive ratio schedules. While break point numbers were higher for corn oil than food pellets before and after streptozotocin treatment, and satiation or extinction reduced break points, there were no differences between the groups. Parameters for a more complex method of assessment of progressive ratio behaviour derived from Killeen’s Mathematical Principles of Reinforcement were also computed. While the parameter associated with incentive value was higher for corn oil than for food pellets, parameters were not significantly affected by streptozotocin treatment. Overall, a range of behavioural measures of food motivation failed to reveal effects of changes relative to controls in mice that showed physiological evidence of type 2 diabetes.

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KW - high-fat diet

KW - streptozotocin treatment

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DO - 10.1016/j.lmot.2021.101730

M3 - Article

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JO - Learning and Motivation

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M1 - 101730

ER -

Behavioural evaluation of mouse models of type 2 diabetes

Abstract

Bibliographical note

Keywords

UN SDGs

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