Bacterial Proteases and Haemostasis Dysregulation in the CF Lung

J.A. Reihill, M. Moreland, G.E. Jarvis, A. McDowell, G.G. Einarsson, J.S. Elborn, S.L. Martin

Research output: Contribution to journalArticle

Abstract

Background: Pathogenic bacteria which chronically colonise the cystic fibrosis (CF) lung produce a number of virulence determinants, including distinct proteolytic activities. The potential role bacterial proteases play on haemostatic dysregulation within the CF lung is, however, poorly defined, despite haemoptysis being a common complication in CF. Methods: The potential impact of known CF pathogens (Pseudomonas aeruginosa and Burkholderia cepacia complex spp.) on haemostasis was examined for their ability to degrade fibrinogen and dysregulate fibrin clot formation and platelet aggregation.Results: Results demonstrate that key CF pathogens growing as a biofilm on mucin exhibit considerable fibrinogenolytic activity, resulting in fibrinogen breakdown, impaired clot formation, and modulation of platelet aggregation. Human neutrophil elastase may also contribute to fibrinogen breakdown and dysregulated clot formation at high concentration.Conclusion: Bacterial-derived proteases may play an important role in the dysregulation of airway haemostasis, and potentially contribute to episodes of haemoptysis within the CF lung.
LanguageEnglish
Pages49-57
Number of pages8
JournalJournal of Cystic Fibrosis
Volume16
Issue number1
Early online date10 Nov 2016
DOIs
Publication statusE-pub ahead of print - 10 Nov 2016

Fingerprint

Hemostasis
Cystic Fibrosis
Peptide Hydrolases
Lung
Fibrinogen
Hemoptysis
Platelet Aggregation
Burkholderia cepacia complex
Burkholderia cepacia
Leukocyte Elastase
Mucins
Hemostatics
Biofilms
Fibrin
Pseudomonas aeruginosa
Virulence
Bacteria

Keywords

  • Cystic fibrosis
  • Haemostasis
  • Haemoptysis
  • Coagulation
  • Platelet aggregation
  • Pseudomonas aeruginosa
  • Burkholderia cenocepacia
  • Burkholderia multivorans

Cite this

Reihill, J. A., Moreland, M., Jarvis, G. E., McDowell, A., Einarsson, G. G., Elborn, J. S., & Martin, S. L. (2016). Bacterial Proteases and Haemostasis Dysregulation in the CF Lung. Journal of Cystic Fibrosis, 16(1), 49-57. https://doi.org/10.1016/j.jcf.2016.10.006
Reihill, J.A. ; Moreland, M. ; Jarvis, G.E. ; McDowell, A. ; Einarsson, G.G. ; Elborn, J.S. ; Martin, S.L. / Bacterial Proteases and Haemostasis Dysregulation in the CF Lung. In: Journal of Cystic Fibrosis. 2016 ; Vol. 16, No. 1. pp. 49-57.
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Reihill, JA, Moreland, M, Jarvis, GE, McDowell, A, Einarsson, GG, Elborn, JS & Martin, SL 2016, 'Bacterial Proteases and Haemostasis Dysregulation in the CF Lung', Journal of Cystic Fibrosis, vol. 16, no. 1, pp. 49-57. https://doi.org/10.1016/j.jcf.2016.10.006

Bacterial Proteases and Haemostasis Dysregulation in the CF Lung. / Reihill, J.A.; Moreland, M.; Jarvis, G.E.; McDowell, A.; Einarsson, G.G.; Elborn, J.S.; Martin, S.L.

In: Journal of Cystic Fibrosis, Vol. 16, No. 1, 10.11.2016, p. 49-57.

Research output: Contribution to journalArticle

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AU - Jarvis, G.E.

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AU - Einarsson, G.G.

AU - Elborn, J.S.

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