Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study

David Gibson, Ji Qiu, Sorcha Finnegan, EA Mendoza, K Barker, C McAllister, Joshua LaBaer, Madeleine Rooney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Method: Nucleic Acid Programmable Protein Array (NAPPA) enables the production of multiple proteins from DNA templates which are immobilized on a solid phase. These can then be probed for the presence of autoantibodies in patient’s serum. NAPPA slides with 2200 genes were produced. PubMed search identified ∼60 genes associated with uveitis pathology and ∼30 genes associated with arthritis development. The remaining ∼2100 genes were randomly identified from a ∼12 000 human gene collection (http://dnasu.asu.edu) (Fig. 1). The arrays were then probed using plasma from JIA patients with (n = 20) and without uveitis (n = 20) and from healthy age and sex matched controls (n = 20). Results: Quantitative reproducibility of NAPPAs was demonstrated with > 0.95 intra-array and inter-array correlations. Differences in the levels of potential autoantibodies were revealed between JIA patients with and without uveitis. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis. Conclusion: The NAPPA platform has the potential to identify novel autoantibodies that robustly forecast the development of uveitis in children with Juvenile idiopathic arthritis. This predictive tool could enable the development of a more appropriate, effective and efficient clinical management algorithm.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pagesiii2
Number of pages2
Volume53
DOIs
Publication statusPublished - 2014
EventBritish Society for Paediatric and Adolescent Rheumatology -
Duration: 1 Jan 2014 → …

Conference

ConferenceBritish Society for Paediatric and Adolescent Rheumatology
Period1/01/14 → …

Fingerprint

Juvenile Arthritis
Uveitis
Autoantibodies
Protein Array Analysis
Nucleic Acids
Genes
PubMed
Reproducibility of Results
Arthritis
Cluster Analysis
Pathology
Antibodies
DNA
Serum
Proteins

Keywords

  • Uveitis
  • Juvenile arthritis
  • autoantibodies

Cite this

Gibson, D., Qiu, J., Finnegan, S., Mendoza, EA., Barker, K., McAllister, C., ... Rooney, M. (2014). Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study. In Unknown Host Publication (Vol. 53, pp. iii2) https://doi.org/10.1093/rheumatology/keu268.003
Gibson, David ; Qiu, Ji ; Finnegan, Sorcha ; Mendoza, EA ; Barker, K ; McAllister, C ; LaBaer, Joshua ; Rooney, Madeleine. / Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study. Unknown Host Publication. Vol. 53 2014. pp. iii2
@inproceedings{ebacaa6983ba491b85d61a0cb9c090a5,
title = "Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study",
abstract = "Method: Nucleic Acid Programmable Protein Array (NAPPA) enables the production of multiple proteins from DNA templates which are immobilized on a solid phase. These can then be probed for the presence of autoantibodies in patient’s serum. NAPPA slides with 2200 genes were produced. PubMed search identified ∼60 genes associated with uveitis pathology and ∼30 genes associated with arthritis development. The remaining ∼2100 genes were randomly identified from a ∼12 000 human gene collection (http://dnasu.asu.edu) (Fig. 1). The arrays were then probed using plasma from JIA patients with (n = 20) and without uveitis (n = 20) and from healthy age and sex matched controls (n = 20). Results: Quantitative reproducibility of NAPPAs was demonstrated with > 0.95 intra-array and inter-array correlations. Differences in the levels of potential autoantibodies were revealed between JIA patients with and without uveitis. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis. Conclusion: The NAPPA platform has the potential to identify novel autoantibodies that robustly forecast the development of uveitis in children with Juvenile idiopathic arthritis. This predictive tool could enable the development of a more appropriate, effective and efficient clinical management algorithm.",
keywords = "Uveitis, Juvenile arthritis, autoantibodies",
author = "David Gibson and Ji Qiu and Sorcha Finnegan and EA Mendoza and K Barker and C McAllister and Joshua LaBaer and Madeleine Rooney",
year = "2014",
doi = "10.1093/rheumatology/keu268.003",
language = "English",
volume = "53",
pages = "iii2",
booktitle = "Unknown Host Publication",

}

Gibson, D, Qiu, J, Finnegan, S, Mendoza, EA, Barker, K, McAllister, C, LaBaer, J & Rooney, M 2014, Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study. in Unknown Host Publication. vol. 53, pp. iii2, British Society for Paediatric and Adolescent Rheumatology, 1/01/14. https://doi.org/10.1093/rheumatology/keu268.003

Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study. / Gibson, David; Qiu, Ji; Finnegan, Sorcha; Mendoza, EA; Barker, K; McAllister, C; LaBaer, Joshua; Rooney, Madeleine.

Unknown Host Publication. Vol. 53 2014. p. iii2.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study

AU - Gibson, David

AU - Qiu, Ji

AU - Finnegan, Sorcha

AU - Mendoza, EA

AU - Barker, K

AU - McAllister, C

AU - LaBaer, Joshua

AU - Rooney, Madeleine

PY - 2014

Y1 - 2014

N2 - Method: Nucleic Acid Programmable Protein Array (NAPPA) enables the production of multiple proteins from DNA templates which are immobilized on a solid phase. These can then be probed for the presence of autoantibodies in patient’s serum. NAPPA slides with 2200 genes were produced. PubMed search identified ∼60 genes associated with uveitis pathology and ∼30 genes associated with arthritis development. The remaining ∼2100 genes were randomly identified from a ∼12 000 human gene collection (http://dnasu.asu.edu) (Fig. 1). The arrays were then probed using plasma from JIA patients with (n = 20) and without uveitis (n = 20) and from healthy age and sex matched controls (n = 20). Results: Quantitative reproducibility of NAPPAs was demonstrated with > 0.95 intra-array and inter-array correlations. Differences in the levels of potential autoantibodies were revealed between JIA patients with and without uveitis. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis. Conclusion: The NAPPA platform has the potential to identify novel autoantibodies that robustly forecast the development of uveitis in children with Juvenile idiopathic arthritis. This predictive tool could enable the development of a more appropriate, effective and efficient clinical management algorithm.

AB - Method: Nucleic Acid Programmable Protein Array (NAPPA) enables the production of multiple proteins from DNA templates which are immobilized on a solid phase. These can then be probed for the presence of autoantibodies in patient’s serum. NAPPA slides with 2200 genes were produced. PubMed search identified ∼60 genes associated with uveitis pathology and ∼30 genes associated with arthritis development. The remaining ∼2100 genes were randomly identified from a ∼12 000 human gene collection (http://dnasu.asu.edu) (Fig. 1). The arrays were then probed using plasma from JIA patients with (n = 20) and without uveitis (n = 20) and from healthy age and sex matched controls (n = 20). Results: Quantitative reproducibility of NAPPAs was demonstrated with > 0.95 intra-array and inter-array correlations. Differences in the levels of potential autoantibodies were revealed between JIA patients with and without uveitis. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis. Conclusion: The NAPPA platform has the potential to identify novel autoantibodies that robustly forecast the development of uveitis in children with Juvenile idiopathic arthritis. This predictive tool could enable the development of a more appropriate, effective and efficient clinical management algorithm.

KW - Uveitis

KW - Juvenile arthritis

KW - autoantibodies

U2 - 10.1093/rheumatology/keu268.003

DO - 10.1093/rheumatology/keu268.003

M3 - Conference contribution

VL - 53

SP - iii2

BT - Unknown Host Publication

ER -

Gibson D, Qiu J, Finnegan S, Mendoza EA, Barker K, McAllister C et al. Autoantibodies Predictive of Uveitis in Juvenile Idiopathic Arthritis (Aperture): a proof of concept study. In Unknown Host Publication. Vol. 53. 2014. p. iii2 https://doi.org/10.1093/rheumatology/keu268.003