Abstract
| Language | English |
|---|---|
| Pages | 171-177 |
| Journal | Gene |
| Volume | 163 |
| Issue number | 2 |
| Publication status | Published - Oct 1995 |
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ASSOCIATION OF AN UNUSUAL FORM OF A PAX7-LIKE GENE WITH INCREASED EFFICIENCY OF SKELETAL-MUSCLE REGENERATION. / KAY, PH; Mitchell, Christopher; AKKARI, A; PAPADIMITRIOU, JM.
In: Gene, Vol. 163, No. 2, 10.1995, p. 171-177.Research output: Contribution to journal › Article
TY - JOUR
T1 - ASSOCIATION OF AN UNUSUAL FORM OF A PAX7-LIKE GENE WITH INCREASED EFFICIENCY OF SKELETAL-MUSCLE REGENERATION
AU - KAY, PH
AU - Mitchell, Christopher
AU - AKKARI, A
AU - PAPADIMITRIOU, JM
PY - 1995/10
Y1 - 1995/10
N2 - Efficiency of regeneration of mechanically injured skeletal muscle is more pronounced in SJL/J mice, as compared to other laboratory strains in which regenerative properties of skeletal muscle are uniformly poor. Previously, we postulated that a small number of genes might differ between SJL/J and; other mouse strains, and would be responsible for this variation in the efficiency of skeletal muscle regeneration. The results of initial experiments demonstrated that SJL/J mice have a unique form of the myogenic gene, Myo-D1, which partly influences efficiency of skeletal muscle repair, and that other genes were also involved. To identify other candidate genes, differences were sought within the myogenic paired box/homeobox-containing gene Pax7 between SJL/J and other laboratory mouse strains. Southern blotting indicated that SJL/J, Quackenbush and DDO mice share a Pax7/TaqI RFLP which differs from all other laboratory strains tested. This RFLP is most likely due to sequence differences within the homeobox of a Pax7-like gene. In vivo studies revealed that Quackenbush and DDO mice also share the same regenerative properties of mechanically damaged skeletal muscle as SJL/J mice. Since Quackenbush and DDO mice lack the SJL/J type of Myo-D1, and DDO belong to a different mouse sub-species, these studies suggest that structural alterations in the homeobox of a Pax7-like gene may be implicated in the effectiveness of renewal of damaged skeletal muscle of the limb in the mature animal.
AB - Efficiency of regeneration of mechanically injured skeletal muscle is more pronounced in SJL/J mice, as compared to other laboratory strains in which regenerative properties of skeletal muscle are uniformly poor. Previously, we postulated that a small number of genes might differ between SJL/J and; other mouse strains, and would be responsible for this variation in the efficiency of skeletal muscle regeneration. The results of initial experiments demonstrated that SJL/J mice have a unique form of the myogenic gene, Myo-D1, which partly influences efficiency of skeletal muscle repair, and that other genes were also involved. To identify other candidate genes, differences were sought within the myogenic paired box/homeobox-containing gene Pax7 between SJL/J and other laboratory mouse strains. Southern blotting indicated that SJL/J, Quackenbush and DDO mice share a Pax7/TaqI RFLP which differs from all other laboratory strains tested. This RFLP is most likely due to sequence differences within the homeobox of a Pax7-like gene. In vivo studies revealed that Quackenbush and DDO mice also share the same regenerative properties of mechanically damaged skeletal muscle as SJL/J mice. Since Quackenbush and DDO mice lack the SJL/J type of Myo-D1, and DDO belong to a different mouse sub-species, these studies suggest that structural alterations in the homeobox of a Pax7-like gene may be implicated in the effectiveness of renewal of damaged skeletal muscle of the limb in the mature animal.
M3 - Article
VL - 163
SP - 171
EP - 177
JO - Gene
T2 - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -