ASSOCIATION OF AN UNUSUAL FORM OF A PAX7-LIKE GENE WITH INCREASED EFFICIENCY OF SKELETAL-MUSCLE REGENERATION

PH KAY, Christopher Mitchell, A AKKARI, JM PAPADIMITRIOU

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Efficiency of regeneration of mechanically injured skeletal muscle is more pronounced in SJL/J mice, as compared to other laboratory strains in which regenerative properties of skeletal muscle are uniformly poor. Previously, we postulated that a small number of genes might differ between SJL/J and; other mouse strains, and would be responsible for this variation in the efficiency of skeletal muscle regeneration. The results of initial experiments demonstrated that SJL/J mice have a unique form of the myogenic gene, Myo-D1, which partly influences efficiency of skeletal muscle repair, and that other genes were also involved. To identify other candidate genes, differences were sought within the myogenic paired box/homeobox-containing gene Pax7 between SJL/J and other laboratory mouse strains. Southern blotting indicated that SJL/J, Quackenbush and DDO mice share a Pax7/TaqI RFLP which differs from all other laboratory strains tested. This RFLP is most likely due to sequence differences within the homeobox of a Pax7-like gene. In vivo studies revealed that Quackenbush and DDO mice also share the same regenerative properties of mechanically damaged skeletal muscle as SJL/J mice. Since Quackenbush and DDO mice lack the SJL/J type of Myo-D1, and DDO belong to a different mouse sub-species, these studies suggest that structural alterations in the homeobox of a Pax7-like gene may be implicated in the effectiveness of renewal of damaged skeletal muscle of the limb in the mature animal.
LanguageEnglish
Pages171-177
JournalGene
Volume163
Issue number2
Publication statusPublished - Oct 1995

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Regeneration
Skeletal Muscle
Genes
Homeobox Genes
Restriction Fragment Length Polymorphisms
Southern Blotting
Extremities

Cite this

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title = "ASSOCIATION OF AN UNUSUAL FORM OF A PAX7-LIKE GENE WITH INCREASED EFFICIENCY OF SKELETAL-MUSCLE REGENERATION",
abstract = "Efficiency of regeneration of mechanically injured skeletal muscle is more pronounced in SJL/J mice, as compared to other laboratory strains in which regenerative properties of skeletal muscle are uniformly poor. Previously, we postulated that a small number of genes might differ between SJL/J and; other mouse strains, and would be responsible for this variation in the efficiency of skeletal muscle regeneration. The results of initial experiments demonstrated that SJL/J mice have a unique form of the myogenic gene, Myo-D1, which partly influences efficiency of skeletal muscle repair, and that other genes were also involved. To identify other candidate genes, differences were sought within the myogenic paired box/homeobox-containing gene Pax7 between SJL/J and other laboratory mouse strains. Southern blotting indicated that SJL/J, Quackenbush and DDO mice share a Pax7/TaqI RFLP which differs from all other laboratory strains tested. This RFLP is most likely due to sequence differences within the homeobox of a Pax7-like gene. In vivo studies revealed that Quackenbush and DDO mice also share the same regenerative properties of mechanically damaged skeletal muscle as SJL/J mice. Since Quackenbush and DDO mice lack the SJL/J type of Myo-D1, and DDO belong to a different mouse sub-species, these studies suggest that structural alterations in the homeobox of a Pax7-like gene may be implicated in the effectiveness of renewal of damaged skeletal muscle of the limb in the mature animal.",
author = "PH KAY and Christopher Mitchell and A AKKARI and JM PAPADIMITRIOU",
year = "1995",
month = "10",
language = "English",
volume = "163",
pages = "171--177",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

ASSOCIATION OF AN UNUSUAL FORM OF A PAX7-LIKE GENE WITH INCREASED EFFICIENCY OF SKELETAL-MUSCLE REGENERATION. / KAY, PH; Mitchell, Christopher; AKKARI, A; PAPADIMITRIOU, JM.

In: Gene, Vol. 163, No. 2, 10.1995, p. 171-177.

Research output: Contribution to journalArticle

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