A screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43±0.029 mN/m and interfacial tension to 0.38±0.03 mN/m at 72 h and an emulsification index (E24) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C–O–C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of ft. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics.
- Mass spectrometry