Assessment of Rheological Behaviour of Water‑in‑Oil Emulsions Mediated by Glycolipid Biosurfactant Produced by Bacillus megaterium SPSW1001

Varsha Singh; Zairah Waris; Ibrahim M Banat; Sriparna Saha; Padmini Padmanabhan

doi:10.1007/s12010-021-03717-3

Assessment of Rheological Behaviour of Water‑in‑Oil Emulsions Mediated by Glycolipid Biosurfactant Produced by Bacillus megaterium SPSW1001

Varsha Singh, Zairah Waris, Ibrahim M Banat, Sriparna Saha, Padmini Padmanabhan

Faculty Of Life & Health Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

104 Downloads (Pure)

Abstract

A screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43 ± 0.029 mN/m and interfacial tension to 0.38 ± 0.03 mN/m at 72 h and an emulsification index (E ) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C-O-C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of fit. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics. [Abstract copyright: © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.]

Original language	English
Pages (from-to)	1310-1326
Number of pages	17
Journal	Applied Biochemistry and Biotechnology
Volume	194
Issue number	3
Early online date	25 Oct 2021
DOIs	https://doi.org/10.1007/s12010-021-03717-3
Publication status	Published (in print/issue) - 31 Mar 2022

Bibliographical note

Funding Information:
We gratefully acknowledge the Government of India (Department of Science & Technology) Scheme for Women Scientist-A (WOS-A) for a research grant (Project no. SR/WOS-A/LS-154/2018) to carry out this work.

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Bacillus
Biosurfactant
Emulsification
Mass spectrometry
Rheology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12010-021-03717-3

ABAB 14.07.21 R1 ManuscriptAuthor Accepted Manuscript, 784 KB

Cite this

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N2 - A screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43 ± 0.029 mN/m and interfacial tension to 0.38 ± 0.03 mN/m at 72 h and an emulsification index (E ) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C-O-C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of fit. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics. [Abstract copyright: © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.]

AB - A screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43 ± 0.029 mN/m and interfacial tension to 0.38 ± 0.03 mN/m at 72 h and an emulsification index (E ) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C-O-C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of fit. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics. [Abstract copyright: © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.]

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ER -

Assessment of Rheological Behaviour of Water‑in‑Oil Emulsions Mediated by Glycolipid Biosurfactant Produced by Bacillus megaterium SPSW1001

Abstract

Bibliographical note

Keywords

UN SDGs

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