A screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43 ± 0.029 mN/m and interfacial tension to 0.38 ± 0.03 mN/m at 72 h and an emulsification index (E ) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C-O-C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of fit. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics. [Abstract copyright: © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.]
|Number of pages||17|
|Journal||Applied Biochemistry and Biotechnology|
|Early online date||25 Oct 2021|
|Publication status||Published (in print/issue) - 31 Mar 2022|
Bibliographical noteFunding Information:
We gratefully acknowledge the Government of India (Department of Science & Technology) Scheme for Women Scientist-A (WOS-A) for a research grant (Project no. SR/WOS-A/LS-154/2018) to carry out this work.
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
- Mass spectrometry