[Arg3]substance P and neurokinin A from chicken small intestine

J. Michael Conlon, Stavros Katsoulis, Wolfgang E. Schmidt, Lars Thim

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26 Citations (Scopus)

Abstract

Using antisera specific for NH2-terminal and COOH-terminal regions of substance P and for the COOH-terminal region of neurokinin A, peptides with tachykinin-like immunoreactivity were isolated from extracts of chicken small intestine. The peptide Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 differs from human substance P by substitution of the lysyl residue by an arginyl residue at position 3. Synthetic [Arg3]substance P showed identical chromatographic and immunochemical properties to chicken substance P and was equipotent with substance P in contracting the guinea pig ileum. A second peptide His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 isolated from the extracts is identical to human neurokinin A. A third peptide was immunoreactive towards the COOH-terminally directed antiserum to substance P only but was not characterized structurally in this study.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalRegulatory Peptides
Volume20
Issue number2
DOIs
Publication statusPublished (in print/issue) - Feb 1988

Keywords

  • Chicken gut
  • HPLC
  • Primary structure
  • Radioimmunoassay
  • Tachykinin

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