ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats

Eun-Mee Kim, E O'Hare, MK Grace, CC Welch, CJ Billington, AS Levine

Research output: Contribution to journalArticle

Abstract

Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and alpha-melanocyte stimulating hormone (alpha-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P > 0.05). Obese Zucker rats had lower alpha-MSH and dynorphin A(1-17) peptide levels in the paraventricular nucleus (PVN) (P < 0.05), but did not have lower PVN beta-endorphin peptide levels (P > 0.05). The decrease in POMC in the ARC and decrease in alpha-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
LanguageEnglish
Pages11-16
JournalBrain Research
Volume862
Issue number1-2
Publication statusPublished - Apr 2000

Fingerprint

Zucker Rats
alpha-MSH
Pro-Opiomelanocortin
Arcuate Nucleus of Hypothalamus
Paraventricular Hypothalamic Nucleus
Messenger RNA
Melanocortins
Opioid Peptides
Leptin
Insulin
Dynorphins
Hyperphagia
Neuropeptide Y
Obesity
Gene Expression
Glucose
Peptides
Serum

Cite this

Kim, E-M., O'Hare, E., Grace, MK., Welch, CC., Billington, CJ., & Levine, AS. (2000). ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats. Brain Research, 862(1-2), 11-16.
Kim, Eun-Mee ; O'Hare, E ; Grace, MK ; Welch, CC ; Billington, CJ ; Levine, AS. / ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats. In: Brain Research. 2000 ; Vol. 862, No. 1-2. pp. 11-16.
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abstract = "Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and alpha-melanocyte stimulating hormone (alpha-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P > 0.05). Obese Zucker rats had lower alpha-MSH and dynorphin A(1-17) peptide levels in the paraventricular nucleus (PVN) (P < 0.05), but did not have lower PVN beta-endorphin peptide levels (P > 0.05). The decrease in POMC in the ARC and decrease in alpha-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. (C) 2000 Published by Elsevier Science B.V. All rights reserved.",
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Kim, E-M, O'Hare, E, Grace, MK, Welch, CC, Billington, CJ & Levine, AS 2000, 'ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats', Brain Research, vol. 862, no. 1-2, pp. 11-16.

ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats. / Kim, Eun-Mee; O'Hare, E; Grace, MK; Welch, CC; Billington, CJ; Levine, AS.

In: Brain Research, Vol. 862, No. 1-2, 04.2000, p. 11-16.

Research output: Contribution to journalArticle

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T1 - ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats

AU - Kim, Eun-Mee

AU - O'Hare, E

AU - Grace, MK

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AU - Billington, CJ

AU - Levine, AS

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N2 - Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and alpha-melanocyte stimulating hormone (alpha-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P > 0.05). Obese Zucker rats had lower alpha-MSH and dynorphin A(1-17) peptide levels in the paraventricular nucleus (PVN) (P < 0.05), but did not have lower PVN beta-endorphin peptide levels (P > 0.05). The decrease in POMC in the ARC and decrease in alpha-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

AB - Effects of obesity on gene expression for opioid peptides and neuropeptide-Y (NPY) in the arcuate nucleus (ARC), and on opioid peptides and alpha-melanocyte stimulating hormone (alpha-MSH) in the paraventricular nucleus (PVN) were examined in obese Zucker rats (18 weeks old). Obese Zucker rats are insulin-resistant, diabetic and hyperleptinemic as indicated by high serum glucose, insulin and leptin levels. ARC proOpiomelanocortin (POMC) mRNA levels were significantly lower in the obese relative to lean Zucker rats and ARC proNeuropeptide Y (proNPY) mRNA levels were higher (P<0.05). There were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (P > 0.05). Obese Zucker rats had lower alpha-MSH and dynorphin A(1-17) peptide levels in the paraventricular nucleus (PVN) (P < 0.05), but did not have lower PVN beta-endorphin peptide levels (P > 0.05). The decrease in POMC in the ARC and decrease in alpha-MSH in the PVN seen in the obese Zucker rat in the present study suggest that reduced activity of the melanocortin system in the ARC to PVN pathway may contribute to the related hyperphagia. Reduced activity of the melanocortin system in the ARC to PVN pathway may be due to a disturbance of leptin signaling coupling to POMC. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

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JO - Brain Research

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SN - 0006-8993

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ER -

Kim E-M, O'Hare E, Grace MK, Welch CC, Billington CJ, Levine AS. ARC POMC mRNA and PVN alpha-MSH are lower in obese relative to lean Zucker rats. Brain Research. 2000 Apr;862(1-2):11-16.