Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin

M McMullan, N Sun, P Papakonstantinou, MX Li, W Zhou, D Mihalilovic

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We demonstrate the use of a novel electrochemical sensing platform based on aptamer conjugated Mo6S9−xIx nanowires (MoSI NWs) for the highly sensitive detection of the blood clotting enzyme thrombin.MoSI NWs nanowires were self-assembled on a gold electrode to which thrombin binding aptamers were covalently attached. Themodification and immobilization steps of the electrodeswere characterised by cyclic voltammetry along with high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The platform is based on the creation of a self-assembled MoSI MWlayer via thesulfur–gold affinity followed by the creation of MoSI-thiolated aptamer conjugates via the sulfur–sulfur affinity. Using this system, sensitive quantitative detection of thrombin is realized by monitoring differences of differential pulse voltammetric responses of electrostatically trapped [Ru(NH3)6]3+ cations to the aptamer before and after thrombin binding. The sensitivity limit for the detection of thrombin is 10 pM. This value is 10-fold better than all currently reported one step label free electrochemical strategies. Given the direct label free nature of the approach and the simplicity of the electronic detection, theaptamer conjugated MoSI NWs biosensor appears well suited for implementation in portable point of care microdevices directed at the rapid and sensitive detection of proteins and pathogens.
LanguageEnglish
Pages1853-1859
JournalBiosensors and Bioelectronics
Volume26
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Nanowires
Thrombin
Sulfur
Gold
Labels
Point-of-Care Systems
Photoelectron Spectroscopy
Biosensing Techniques
Blood Coagulation
Pathogens
High resolution transmission electron microscopy
Transmission Electron Microscopy
Biosensors
Immobilization
Cyclic voltammetry
Limit of Detection
Cations
Electrodes
Blood
X ray photoelectron spectroscopy

Cite this

McMullan, M ; Sun, N ; Papakonstantinou, P ; Li, MX ; Zhou, W ; Mihalilovic, D. / Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin. 2011 ; Vol. 26. pp. 1853-1859.
@article{867fdf1290e144299bf7a4cb586b2030,
title = "Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin",
abstract = "We demonstrate the use of a novel electrochemical sensing platform based on aptamer conjugated Mo6S9−xIx nanowires (MoSI NWs) for the highly sensitive detection of the blood clotting enzyme thrombin.MoSI NWs nanowires were self-assembled on a gold electrode to which thrombin binding aptamers were covalently attached. Themodification and immobilization steps of the electrodeswere characterised by cyclic voltammetry along with high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The platform is based on the creation of a self-assembled MoSI MWlayer via thesulfur–gold affinity followed by the creation of MoSI-thiolated aptamer conjugates via the sulfur–sulfur affinity. Using this system, sensitive quantitative detection of thrombin is realized by monitoring differences of differential pulse voltammetric responses of electrostatically trapped [Ru(NH3)6]3+ cations to the aptamer before and after thrombin binding. The sensitivity limit for the detection of thrombin is 10 pM. This value is 10-fold better than all currently reported one step label free electrochemical strategies. Given the direct label free nature of the approach and the simplicity of the electronic detection, theaptamer conjugated MoSI NWs biosensor appears well suited for implementation in portable point of care microdevices directed at the rapid and sensitive detection of proteins and pathogens.",
author = "M McMullan and N Sun and P Papakonstantinou and MX Li and W Zhou and D Mihalilovic",
year = "2011",
month = "1",
doi = "10.1016/j.bios.2010.01.030",
language = "English",
volume = "26",
pages = "1853--1859",

}

Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin. / McMullan, M; Sun, N; Papakonstantinou, P; Li, MX; Zhou, W; Mihalilovic, D.

Vol. 26, 01.2011, p. 1853-1859.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin

AU - McMullan, M

AU - Sun, N

AU - Papakonstantinou, P

AU - Li, MX

AU - Zhou, W

AU - Mihalilovic, D

PY - 2011/1

Y1 - 2011/1

N2 - We demonstrate the use of a novel electrochemical sensing platform based on aptamer conjugated Mo6S9−xIx nanowires (MoSI NWs) for the highly sensitive detection of the blood clotting enzyme thrombin.MoSI NWs nanowires were self-assembled on a gold electrode to which thrombin binding aptamers were covalently attached. Themodification and immobilization steps of the electrodeswere characterised by cyclic voltammetry along with high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The platform is based on the creation of a self-assembled MoSI MWlayer via thesulfur–gold affinity followed by the creation of MoSI-thiolated aptamer conjugates via the sulfur–sulfur affinity. Using this system, sensitive quantitative detection of thrombin is realized by monitoring differences of differential pulse voltammetric responses of electrostatically trapped [Ru(NH3)6]3+ cations to the aptamer before and after thrombin binding. The sensitivity limit for the detection of thrombin is 10 pM. This value is 10-fold better than all currently reported one step label free electrochemical strategies. Given the direct label free nature of the approach and the simplicity of the electronic detection, theaptamer conjugated MoSI NWs biosensor appears well suited for implementation in portable point of care microdevices directed at the rapid and sensitive detection of proteins and pathogens.

AB - We demonstrate the use of a novel electrochemical sensing platform based on aptamer conjugated Mo6S9−xIx nanowires (MoSI NWs) for the highly sensitive detection of the blood clotting enzyme thrombin.MoSI NWs nanowires were self-assembled on a gold electrode to which thrombin binding aptamers were covalently attached. Themodification and immobilization steps of the electrodeswere characterised by cyclic voltammetry along with high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The platform is based on the creation of a self-assembled MoSI MWlayer via thesulfur–gold affinity followed by the creation of MoSI-thiolated aptamer conjugates via the sulfur–sulfur affinity. Using this system, sensitive quantitative detection of thrombin is realized by monitoring differences of differential pulse voltammetric responses of electrostatically trapped [Ru(NH3)6]3+ cations to the aptamer before and after thrombin binding. The sensitivity limit for the detection of thrombin is 10 pM. This value is 10-fold better than all currently reported one step label free electrochemical strategies. Given the direct label free nature of the approach and the simplicity of the electronic detection, theaptamer conjugated MoSI NWs biosensor appears well suited for implementation in portable point of care microdevices directed at the rapid and sensitive detection of proteins and pathogens.

U2 - 10.1016/j.bios.2010.01.030

DO - 10.1016/j.bios.2010.01.030

M3 - Article

VL - 26

SP - 1853

EP - 1859

ER -