Aptamer-based field-effect biosensor for tenofovir detection

N. Aliakbarinodehi, P. Jolly, N. Bhalla, A. Miodek, G. De Micheli, P. Estrela, S. Carrara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.

LanguageEnglish
Article number44409
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 15 Mar 2017

Fingerprint

Tenofovir
Biosensing Techniques
Drug Monitoring
Pharmaceutical Preparations
Point-of-Care Systems
Dielectric Spectroscopy
Surface Plasmon Resonance
Transducers
Electric Impedance
Limit of Detection
Buffers
Phosphates
Serum

Cite this

Aliakbarinodehi, N., Jolly, P., Bhalla, N., Miodek, A., De Micheli, G., Estrela, P., & Carrara, S. (2017). Aptamer-based field-effect biosensor for tenofovir detection. Scientific Reports, 7, [44409]. https://doi.org/10.1038/srep44409
Aliakbarinodehi, N. ; Jolly, P. ; Bhalla, N. ; Miodek, A. ; De Micheli, G. ; Estrela, P. ; Carrara, S. / Aptamer-based field-effect biosensor for tenofovir detection. In: Scientific Reports. 2017 ; Vol. 7.
@article{484663400e354b0998ce4c2a2037867d,
title = "Aptamer-based field-effect biosensor for tenofovir detection",
abstract = "During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.",
author = "N. Aliakbarinodehi and P. Jolly and N. Bhalla and A. Miodek and {De Micheli}, G. and P. Estrela and S. Carrara",
note = "Dr Bhalla was not employed at Ulster at the time of publication - this paper was published open access and according to Sherpa REF the deposit requirement is therefore waived for this paper (see screenshot attached).",
year = "2017",
month = "3",
day = "15",
doi = "10.1038/srep44409",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",

}

Aliakbarinodehi, N, Jolly, P, Bhalla, N, Miodek, A, De Micheli, G, Estrela, P & Carrara, S 2017, 'Aptamer-based field-effect biosensor for tenofovir detection', Scientific Reports, vol. 7, 44409. https://doi.org/10.1038/srep44409

Aptamer-based field-effect biosensor for tenofovir detection. / Aliakbarinodehi, N.; Jolly, P.; Bhalla, N.; Miodek, A.; De Micheli, G.; Estrela, P.; Carrara, S.

In: Scientific Reports, Vol. 7, 44409, 15.03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aptamer-based field-effect biosensor for tenofovir detection

AU - Aliakbarinodehi, N.

AU - Jolly, P.

AU - Bhalla, N.

AU - Miodek, A.

AU - De Micheli, G.

AU - Estrela, P.

AU - Carrara, S.

N1 - Dr Bhalla was not employed at Ulster at the time of publication - this paper was published open access and according to Sherpa REF the deposit requirement is therefore waived for this paper (see screenshot attached).

PY - 2017/3/15

Y1 - 2017/3/15

N2 - During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.

AB - During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.

UR - http://www.scopus.com/inward/record.url?scp=85015303439&partnerID=8YFLogxK

U2 - 10.1038/srep44409

DO - 10.1038/srep44409

M3 - Article

VL - 7

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 44409

ER -

Aliakbarinodehi N, Jolly P, Bhalla N, Miodek A, De Micheli G, Estrela P et al. Aptamer-based field-effect biosensor for tenofovir detection. Scientific Reports. 2017 Mar 15;7. 44409. https://doi.org/10.1038/srep44409