Application of Liquid Chromatography−Tandem Mass SpectrometryTo Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes?

Research output: Other contribution


Although the use of low-calorie sweeteners (LCSs) is widespread, methods of assessing consumption within free-living populations have inherent limitations. Five commonly consumed LCSs, namely, acesulfame-K, saccharin, sucralose, cyclamate, and steviol glycosides, are excreted via the urine, and therefore a urinary biomarker approach may provide more objective LCS intake data. A LC-ESI-MS/MS method of simultaneously determining acesulfame-K, saccharin, sucralose, cyclamate, and the excretory metabolite of steviol glycosides, steviol glucuronide, in human urine was developed and validated. Linearity was observed over a concentration range of 10−1000 ng/mL with coefficients of determination ranging from 0.9969 to0.9997. Accuracy ranged from 92 to 104%, and intrabatch and interday precisions were within acceptable limits with %CV below 8% for all compounds. A double-blind, randomized crossover dose−response study was conducted to assess the usefulness of urinary LCS excretions (from both fasting spot and a full 24-h urine collection) for investigating recent intakes. Both modes ofsampling were useful for distinguishing between the three short-term intakes of acesulfame-K, saccharin, cyclamates, and steviol glycosides (p <0.001), whereas for sucralose, urinary concentrations were useful for distinguishing between low (0.1% ADI) and high doses (10% ADI) only (p <0.001). In summary, this biomarker approach may be useful for assessing intakes of fivecommonly consumed LCSs.
Original languageEnglish
TypeThe item being deposited is an original research paper describing the development and validation of an analytical method for the simultaneous analysis of five low-calorie sweeteners in urine as well as details on validation of urinary excretions as m...
Publication statusE-pub ahead of print - 16 May 2017



  • low-calorie sweeteners
  • intense sweeteners
  • biomarkers
  • urinary biomarkers
  • exposure
  • food additives
  • human urine

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