Application of Liquid Chromatography−Tandem Mass Spectrometry To Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes?

Research output: Contribution to journalArticle

10 Citations (Scopus)
12 Downloads (Pure)

Abstract

Although the use of low-calorie sweeteners (LCSs) is widespread, methods of assessing consumption within free-living populations have inherent limitations. Five commonly consumed LCSs, namely, acesulfame-K, saccharin, sucralose, cyclamate, and steviol glycosides, are excreted via the urine, and therefore a urinary biomarker approach may provide more objective LCS intake data. A LC-ESI-MS/MS method of simultaneously determining acesulfame-K, saccharin, sucralose, cyclamate, and the excretory metabolite of steviol glycosides, steviol glucuronide, in human urine was developed and validated. Linearity was observed over a concentration range of 10−1000 ng/mL with coefficients of determination ranging from 0.9969 to0.9997. Accuracy ranged from 92 to 104%, and intrabatch and interday precisions were within acceptable limits with %CV below 8% for all compounds. A double-blind, randomized crossover dose−response study was conducted to assess the usefulness of urinary LCS excretions (from both fasting spot and a full 24-h urine collection) for investigating recent intakes. Both modes ofsampling were useful for distinguishing between the three short-term intakes of acesulfame-K, saccharin, cyclamates, and steviol glycosides (p <0.001), whereas for sucralose, urinary concentrations were useful for distinguishing between low (0.1% ADI) and high doses (10% ADI) only (p <0.001). In summary, this biomarker approach may be useful for assessing intakes of fivecommonly consumed LCSs.
Original languageEnglish
Pages (from-to)4516–4525
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number22
Early online date24 May 2017
DOIs
Publication statusPublished - 7 Jun 2017

Keywords

  • low-calorie sweeteners
  • intense sweeteners
  • biomarkers
  • urinary biomarkers
  • exposure
  • food additives
  • human urine

Fingerprint Dive into the research topics of 'Application of Liquid Chromatography−Tandem Mass Spectrometry To Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes?'. Together they form a unique fingerprint.

Cite this