The purpose of this research was to test the accuracy of the picrate screening assay (PSA) in the evaluation of the cyanogenic potential of cassava roots at a remote held site under conditions of unusual difficulty. To do this, a PSA was conducted in the field and compared to data collect-ed previously on five of the same cassava varieties using the Cooke colorimetric enzymic assay. PSA data were collected for 10 different cassava varieties in the Tukanoan Indian village of Vapu Tn the Colombian Amazon region. The PSA results agree with Tukanoan classifications of cassava; that Is, those classified as `sweet' by the Tukanoans generally had low-to-moderate parenchymal cyanogenic potential (0-50 mg kg(-1) fwb of HCN), while those that they classify as `bitter' had high parenchymal cyanogenic potential (greater than or equal to 100 mg kg(-1) fwb of HCN). The PSA results also agree with the data collect-ed using the Cooke colorimetric enzymic assay. The data suggest that the PSA is an appropriate test of the cyanogenic potential of cassava roots in remote field sites. (C) 2000 Society of Chemical Industry.
title = "Application of a picrate semi-quantitative screening assay for the cyanogenic potential of cassava roots at a remote field site",
abstract = "The purpose of this research was to test the accuracy of the picrate screening assay (PSA) in the evaluation of the cyanogenic potential of cassava roots at a remote held site under conditions of unusual difficulty. To do this, a PSA was conducted in the field and compared to data collect-ed previously on five of the same cassava varieties using the Cooke colorimetric enzymic assay. PSA data were collected for 10 different cassava varieties in the Tukanoan Indian village of Vapu Tn the Colombian Amazon region. The PSA results agree with Tukanoan classifications of cassava; that Is, those classified as `sweet' by the Tukanoans generally had low-to-moderate parenchymal cyanogenic potential (0-50 mg kg(-1) fwb of HCN), while those that they classify as `bitter' had high parenchymal cyanogenic potential (greater than or equal to 100 mg kg(-1) fwb of HCN). The PSA results also agree with the data collect-ed using the Cooke colorimetric enzymic assay. The data suggest that the PSA is an appropriate test of the cyanogenic potential of cassava roots in remote field sites. (C) 2000 Society of Chemical Industry.",
author = "WM Wilson and Gerard O'Brien and DL Dufour",
year = "2000",
month = "4",
language = "English",
volume = "80",
pages = "590--594",
journal = "Journal of the Science of Food and Agriculture",
Research output: Contribution to journal › Article
TY - JOUR
T1 - Application of a picrate semi-quantitative screening assay for the cyanogenic potential of cassava roots at a remote field site
AU - Wilson, WM
AU - O'Brien, Gerard
AU - Dufour, DL
PY - 2000/4
Y1 - 2000/4
N2 - The purpose of this research was to test the accuracy of the picrate screening assay (PSA) in the evaluation of the cyanogenic potential of cassava roots at a remote held site under conditions of unusual difficulty. To do this, a PSA was conducted in the field and compared to data collect-ed previously on five of the same cassava varieties using the Cooke colorimetric enzymic assay. PSA data were collected for 10 different cassava varieties in the Tukanoan Indian village of Vapu Tn the Colombian Amazon region. The PSA results agree with Tukanoan classifications of cassava; that Is, those classified as `sweet' by the Tukanoans generally had low-to-moderate parenchymal cyanogenic potential (0-50 mg kg(-1) fwb of HCN), while those that they classify as `bitter' had high parenchymal cyanogenic potential (greater than or equal to 100 mg kg(-1) fwb of HCN). The PSA results also agree with the data collect-ed using the Cooke colorimetric enzymic assay. The data suggest that the PSA is an appropriate test of the cyanogenic potential of cassava roots in remote field sites. (C) 2000 Society of Chemical Industry.
AB - The purpose of this research was to test the accuracy of the picrate screening assay (PSA) in the evaluation of the cyanogenic potential of cassava roots at a remote held site under conditions of unusual difficulty. To do this, a PSA was conducted in the field and compared to data collect-ed previously on five of the same cassava varieties using the Cooke colorimetric enzymic assay. PSA data were collected for 10 different cassava varieties in the Tukanoan Indian village of Vapu Tn the Colombian Amazon region. The PSA results agree with Tukanoan classifications of cassava; that Is, those classified as `sweet' by the Tukanoans generally had low-to-moderate parenchymal cyanogenic potential (0-50 mg kg(-1) fwb of HCN), while those that they classify as `bitter' had high parenchymal cyanogenic potential (greater than or equal to 100 mg kg(-1) fwb of HCN). The PSA results also agree with the data collect-ed using the Cooke colorimetric enzymic assay. The data suggest that the PSA is an appropriate test of the cyanogenic potential of cassava roots in remote field sites. (C) 2000 Society of Chemical Industry.
M3 - Article
VL - 80
SP - 590
EP - 594
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture