Abstract
In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of
2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.
2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.
| Original language | English |
|---|---|
| Pages (from-to) | 1229-1233 |
| Number of pages | 5 |
| Journal | Current Science |
| Volume | 116 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published (in print/issue) - 2019 |
Keywords
- Apoptosis
- repair kinetics
- primary human fibroblasts
- ionizing radiation
- cold atmospheric plasma