Apoptosis time window induced by cold atmospheric plasma: comparison with ionizing radiation

Gordana Joksić; Ana Valenta Šobot; Jelena Filipović Tričković; Dejan Maletić; Nevena  Puač; Gordana Malović; Zoran Petrovic; Saša Lazović

doi:10.18520/cs/v116/i7/1229-1233

Apoptosis time window induced by cold atmospheric plasma: comparison with ionizing radiation

Gordana Joksić, Ana Valenta Šobot, Jelena Filipović Tričković, Dejan Maletić, Nevena Puač, Gordana Malović, Zoran Petrovic, Saša Lazović

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of
2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

Original language	English
Pages (from-to)	1229-1233
Number of pages	5
Journal	Current Science
Volume	116
Issue number	7
DOIs	https://doi.org/10.18520/cs/v116/i7/1229-1233
Publication status	Published (in print/issue) - 2019

Keywords

Apoptosis
repair kinetics
primary human fibroblasts
ionizing radiation
cold atmospheric plasma

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10.18520/cs/v116/i7/1229-1233

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title = "Apoptosis time window induced by cold atmospheric plasma: comparison with ionizing radiation",

abstract = "In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.",

keywords = "Apoptosis, repair kinetics, primary human fibroblasts, ionizing radiation, cold atmospheric plasma",

author = "Gordana Joksi{\'c} and {Valenta {\v S}obot}, Ana and {Filipovi{\'c} Tri{\v c}kovi{\'c}}, Jelena and Dejan Maleti{\'c} and Nevena Pua{\v c} and Gordana Malovi{\'c} and Zoran Petrovic and Sa{\v s}a Lazovi{\'c}",

year = "2019",

doi = "10.18520/cs/v116/i7/1229-1233",

language = "English",

volume = "116",

pages = "1229--1233",

journal = "Current Science",

issn = "0011-3891",

publisher = "Indian Academy of Sciences",

number = "7",

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TY - JOUR

T1 - Apoptosis time window induced by cold atmospheric plasma: comparison with ionizing radiation

AU - Joksić, Gordana

AU - Valenta Šobot, Ana

AU - Filipović Tričković, Jelena

AU - Maletić, Dejan

AU - Puač, Nevena

AU - Malović, Gordana

AU - Petrovic, Zoran

AU - Lazović, Saša

PY - 2019

Y1 - 2019

N2 - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

AB - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

KW - Apoptosis

KW - repair kinetics

KW - primary human fibroblasts

KW - ionizing radiation

KW - cold atmospheric plasma

U2 - 10.18520/cs/v116/i7/1229-1233

DO - 10.18520/cs/v116/i7/1229-1233

M3 - Article

SN - 0011-3891

VL - 116

SP - 1229

EP - 1233

JO - Current Science

JF - Current Science

IS - 7

ER -

Apoptosis time window induced by cold atmospheric plasma: comparison with ionizing radiation

Abstract

Keywords

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