Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components

Muhammad Yasir, Bushra Sultana, P Nigam, Richard Owusu-Apenten

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cucurbitaceae are one of most widely used plant species for human food but lesser known members have not been examined for bioactive components. The purpose of this study was to evaluate the antioxidant and genoprotective activities from three cucurbitaceae seeds extracts and to identify phenolic components by LC–ESIMS/MS analysis. From the results, the yield of seeds extract was 20–41% (w/w) and samples had 16–40% total phenols as gallic acid equivalents (GAE). Compared with methanol solvent, using acidified methanol led to increased extraction yield by 1.4 to 10-fold, higher phenolic content (149.5 ± 1.2 to 396.4 ± 1.9 mg GAE/g), higher DPPH radical quenching and enhanced genoprotective activity using the pBR322 plasmid assay. LC–ESI-MS/MS analysis led to identification of 14–17 components, based on authentic standards and comparison with literature reports, as mainly phenolic acids and esters, flavonol glycosides. This may be the first mass spectrometric profiling of polyphenol components from cucurbitaceae seeds.
LanguageEnglish
Pages307-313
Number of pages12
JournalFood Chemistry
Volume199
DOIs
Publication statusPublished - 2016

Fingerprint

Cucurbitaceae
seed extracts
Seed
Seeds
Gallic Acid
Antioxidants
gallic acid
antioxidants
Methanol
methanol
Phenols
Polyphenols
Glycosides
flavonols
phenolic acids
phenols
glycosides
Quenching
Assays
polyphenols

Keywords

  • Cucurbitaceae seeds
  • Total phenols
  • Phenolic glycosides
  • Genoprotective
  • Antioxidant
  • pBR322 plasmid
  • LC–ESI-MS/MS analysis

Cite this

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title = "Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components",
abstract = "Cucurbitaceae are one of most widely used plant species for human food but lesser known members have not been examined for bioactive components. The purpose of this study was to evaluate the antioxidant and genoprotective activities from three cucurbitaceae seeds extracts and to identify phenolic components by LC–ESIMS/MS analysis. From the results, the yield of seeds extract was 20–41{\%} (w/w) and samples had 16–40{\%} total phenols as gallic acid equivalents (GAE). Compared with methanol solvent, using acidified methanol led to increased extraction yield by 1.4 to 10-fold, higher phenolic content (149.5 ± 1.2 to 396.4 ± 1.9 mg GAE/g), higher DPPH radical quenching and enhanced genoprotective activity using the pBR322 plasmid assay. LC–ESI-MS/MS analysis led to identification of 14–17 components, based on authentic standards and comparison with literature reports, as mainly phenolic acids and esters, flavonol glycosides. This may be the first mass spectrometric profiling of polyphenol components from cucurbitaceae seeds.",
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Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components. / Yasir, Muhammad; Sultana, Bushra; Nigam, P; Owusu-Apenten, Richard.

Vol. 199, 2016, p. 307-313.

Research output: Contribution to journalArticle

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N1 - HighlightsHighlights •Cucurbitaceae seeds are novel sources of flavonol glycosides. •Cucurbitaceae seeds are sources of phenolic acid and esters. •Acidification improves aqueous methanol recovery of cucurbitaceae glycosides. •Extracts from Cucurbitaceae seeds possess antioxidant and genoprotective activity.

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