Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells

T Cheung, Poonam Singh - Nee Nigam, Richard Owusu-Apenten

Research output: Contribution to journalArticle

Abstract

Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid α-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers.Study Design: In-vitro testing.Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10% FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity.Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments.Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of α-Linolenic acid towards MCF-7 breast cancer cells.
LanguageEnglish
Pages1-12
JournalJournal of Applied Life Sciences International
Volume4
Issue number3
DOIs
Publication statusPublished - 8 Jan 2016

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Azadirachta
Curcumin
alpha-Linolenic Acid
Powders
Antioxidants
Breast Neoplasms
Acids
MCF-7 Cells
lissamine rhodamine B
Essential Fatty Acids
Sulfonic Acids
Neoplasms
Cell Survival
Therapeutics
Cell Proliferation

Keywords

  • Antioxidant
  • total phenols
  • curcumin
  • neem (Azadirachta indica)
  • linolenic acid
  • MCF-7 cell
  • anticancer.

Cite this

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title = "Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells",
abstract = "Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid α-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers.Study Design: In-vitro testing.Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10{\%} FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity.Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments.Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of α-Linolenic acid towards MCF-7 breast cancer cells.",
keywords = "Antioxidant, total phenols, curcumin, neem (Azadirachta indica), linolenic acid, MCF-7 cell, anticancer.",
author = "T Cheung and {Singh - Nee Nigam}, Poonam and Richard Owusu-Apenten",
year = "2016",
month = "1",
day = "8",
doi = "10.9734/JALSI/2016/22273",
language = "English",
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Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells. / Cheung, T; Singh - Nee Nigam, Poonam; Owusu-Apenten, Richard.

Vol. 4, No. 3, 08.01.2016, p. 1-12.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells

AU - Cheung, T

AU - Singh - Nee Nigam, Poonam

AU - Owusu-Apenten, Richard

PY - 2016/1/8

Y1 - 2016/1/8

N2 - Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid α-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers.Study Design: In-vitro testing.Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10% FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity.Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments.Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of α-Linolenic acid towards MCF-7 breast cancer cells.

AB - Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid α-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers.Study Design: In-vitro testing.Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10% FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity.Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments.Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of α-Linolenic acid towards MCF-7 breast cancer cells.

KW - Antioxidant

KW - total phenols

KW - curcumin

KW - neem (Azadirachta indica)

KW - linolenic acid

KW - MCF-7 cell

KW - anticancer.

UR - http://sciencedomain.org/abstract/12881

U2 - 10.9734/JALSI/2016/22273

DO - 10.9734/JALSI/2016/22273

M3 - Article

VL - 4

SP - 1

EP - 12

IS - 3

ER -