TY - JOUR
T1 - Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae)
AU - Conlon, J. Michael
AU - Meetani, Mohammed A.
AU - Coquet, Laurent
AU - Jouenne, Thierry
AU - Leprince, Jérôme
AU - Vaudry, Hubert
AU - Kolodziejek, Jolanta
AU - Nowotny, Norbert
AU - King, Jay D.
PY - 2009/10
Y1 - 2009/10
N2 - Taxonomic revisions within the anuran family Ranidae have established the genus Lithobates that currently comprises 49 species of frogs from the New World. Peptidomic analysis, using reversed-phase HPLC with on-line detection by electrospray mass spectrometry, has led to the identification of multiple antimicrobial peptides in norepinephrine-stimulated skin secretions of the North American frog Lithobates capito and the Central American frog Lithobates warszewitschii. Structural characterization of the peptides demonstrated that the L. capito secretions contained brevinin-1 (1), esculentin-1 (1), esculentin-2 (1), ranatuerin-2 (3), and temporin (2) peptides. L. warszewitschii secretions contained brevinin-1 (1), esculentin-2 (1), ranatuerin-2 (2), and temporin (1) peptides. Values in parentheses indicate number of peptides in each family. Temporin-CPa from L. capito, with the atypical structure IPPFIKKVLTTVF·NH2, also showed atypical growth-inhibitory activity having greater potency against Escherichia coli (MIC = 25 μM) and Candida albicans (MIC = 25 μM) than against Staphylococcus aureus (MIC = 50 μM). Phylogenetic analysis based upon the amino acid sequences of 37 ranatuerin-2 peptides from 17 species belonging to the genus Lithobates provides support for currently accepted taxonomic relationships. L. capito is sister-group to Lithobates sevosus in a clade that also contains Lithobates areolatus, and Lithobates palustris. L. warszewitschii is most closely related to the Central American species Lithobates tarahumarae and Lithobates vaillanti.
AB - Taxonomic revisions within the anuran family Ranidae have established the genus Lithobates that currently comprises 49 species of frogs from the New World. Peptidomic analysis, using reversed-phase HPLC with on-line detection by electrospray mass spectrometry, has led to the identification of multiple antimicrobial peptides in norepinephrine-stimulated skin secretions of the North American frog Lithobates capito and the Central American frog Lithobates warszewitschii. Structural characterization of the peptides demonstrated that the L. capito secretions contained brevinin-1 (1), esculentin-1 (1), esculentin-2 (1), ranatuerin-2 (3), and temporin (2) peptides. L. warszewitschii secretions contained brevinin-1 (1), esculentin-2 (1), ranatuerin-2 (2), and temporin (1) peptides. Values in parentheses indicate number of peptides in each family. Temporin-CPa from L. capito, with the atypical structure IPPFIKKVLTTVF·NH2, also showed atypical growth-inhibitory activity having greater potency against Escherichia coli (MIC = 25 μM) and Candida albicans (MIC = 25 μM) than against Staphylococcus aureus (MIC = 50 μM). Phylogenetic analysis based upon the amino acid sequences of 37 ranatuerin-2 peptides from 17 species belonging to the genus Lithobates provides support for currently accepted taxonomic relationships. L. capito is sister-group to Lithobates sevosus in a clade that also contains Lithobates areolatus, and Lithobates palustris. L. warszewitschii is most closely related to the Central American species Lithobates tarahumarae and Lithobates vaillanti.
KW - Antimicrobial peptide
KW - Frog skin
KW - Lithobates
KW - Ranatuerin-2
KW - Temporin
UR - http://www.scopus.com/inward/record.url?scp=70349319361&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2009.07.011
DO - 10.1016/j.peptides.2009.07.011
M3 - Article
C2 - 19635516
AN - SCOPUS:70349319361
SN - 0196-9781
VL - 30
SP - 1775
EP - 1781
JO - Peptides
JF - Peptides
IS - 10
ER -