Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The project tested whether freshwater sponge extracts had antimicrobial properties, which affect the growth of nosocomial bacteria. Furthermore, it investigated if antimicrobial properties of the extracts originated from sponges or their microbiome. Two freshwater sponge species Spongilla lacustris and Ephydatia fluviatilis were tested for antimicrobial properties. Sponges of each species were collected from rivers (full microbiome) and laboratory grown in sterile water (reduced microbiome). Sponge samples were dried and extracted in methanol. After evaporation of methanol extracts were resuspended in DMSO. Antimicrobial properties were assessed by disc diffusion and recording the minimal inhibitory zone (MIZ) for the bacteria Acinetobacter baumanniii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis.None of the tested sponge extracts suppressed the growth of P. aeruginosa, S. aureus or S. epidermidis. Only K. pneumoniae was inhibited by all extracts, with the extracts from laboratory grown sponges resulting in significantly wider MIZs (U=73-89, p=0.004-0.019). A. baumanniii, E. coli and E. faecalis were only inhibited by the extract from riverine S. lacustris. Freshwater sponges are potential sources of antimicrobial compounds, particularly against K. pneumoniae. The antimicrobial properties of laboratory grown sponges suggested that the bioactive molecules which inhibited K. pneumoniae originated from the sponge. However, the enhanced antimicrobial properties of S. lacustris from rivers indicated that there was also a contribution by the microbiome to these extracts’ inhibitory efficacy. Successful clinical therapies against nosocomial bacteria with multidrug resistance will increasingly require new antimicrobial compounds. This study has provided evidence that freshwater sponges contain bioactive molecules. The latter do inhibit bacterial growth and therefore could be a source of antimicrobial compounds, which may originate both from the sponges and their microbiome.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages21-22
Number of pages2
Publication statusAccepted/In press - 14 Mar 2018
EventSfAM 7th ECS research symposium - Birmingham
Duration: 14 Mar 2018 → …

Conference

ConferenceSfAM 7th ECS research symposium
Period14/03/18 → …

Fingerprint

Porifera
Fresh Water
Microbiota
Klebsiella pneumoniae
Staphylococcus epidermidis
Enterococcus faecalis
Bacteria
Rivers
Pseudomonas aeruginosa
Methanol
Staphylococcus aureus
Growth
Escherichia coli
Acinetobacter
Multiple Drug Resistance
Dimethyl Sulfoxide

Keywords

  • Porifera
  • freshwater sponges
  • antibiotic resistance
  • antimicrobial resistance

Cite this

@inproceedings{54236a58cbfd4123ba30725fb773aeb0,
title = "Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges",
abstract = "The project tested whether freshwater sponge extracts had antimicrobial properties, which affect the growth of nosocomial bacteria. Furthermore, it investigated if antimicrobial properties of the extracts originated from sponges or their microbiome. Two freshwater sponge species Spongilla lacustris and Ephydatia fluviatilis were tested for antimicrobial properties. Sponges of each species were collected from rivers (full microbiome) and laboratory grown in sterile water (reduced microbiome). Sponge samples were dried and extracted in methanol. After evaporation of methanol extracts were resuspended in DMSO. Antimicrobial properties were assessed by disc diffusion and recording the minimal inhibitory zone (MIZ) for the bacteria Acinetobacter baumanniii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis.None of the tested sponge extracts suppressed the growth of P. aeruginosa, S. aureus or S. epidermidis. Only K. pneumoniae was inhibited by all extracts, with the extracts from laboratory grown sponges resulting in significantly wider MIZs (U=73-89, p=0.004-0.019). A. baumanniii, E. coli and E. faecalis were only inhibited by the extract from riverine S. lacustris. Freshwater sponges are potential sources of antimicrobial compounds, particularly against K. pneumoniae. The antimicrobial properties of laboratory grown sponges suggested that the bioactive molecules which inhibited K. pneumoniae originated from the sponge. However, the enhanced antimicrobial properties of S. lacustris from rivers indicated that there was also a contribution by the microbiome to these extracts’ inhibitory efficacy. Successful clinical therapies against nosocomial bacteria with multidrug resistance will increasingly require new antimicrobial compounds. This study has provided evidence that freshwater sponges contain bioactive molecules. The latter do inhibit bacterial growth and therefore could be a source of antimicrobial compounds, which may originate both from the sponges and their microbiome.",
keywords = "Porifera, freshwater sponges, antibiotic resistance, antimicrobial resistance",
author = "Allison Cartwright and Joerg Arnscheidt and James Dooley and Chris McGonigle",
note = "Reference text: Marinho, PR, Muricy, GRS, Silva, MFL, Marval, MGDL, Lamport, MS (2010). Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges. Revista Brasileira de Farmacognosia, 20, 267-275. Pejin, B, Talevska, A, Ciric, A, Glamoclija, J, Nikolic, M, Talevski, T (2014). Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. Natural Product Research, 28(24), 2330–3.",
year = "2018",
month = "3",
day = "14",
language = "English",
pages = "21--22",
booktitle = "Unknown Host Publication",

}

Cartwright, A, Arnscheidt, J, Dooley, J & McGonigle, C 2018, Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges. in Unknown Host Publication. pp. 21-22, SfAM 7th ECS research symposium, 14/03/18.

Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges. / Cartwright, Allison; Arnscheidt, Joerg; Dooley, James; McGonigle, Chris.

Unknown Host Publication. 2018. p. 21-22.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges

AU - Cartwright, Allison

AU - Arnscheidt, Joerg

AU - Dooley, James

AU - McGonigle, Chris

N1 - Reference text: Marinho, PR, Muricy, GRS, Silva, MFL, Marval, MGDL, Lamport, MS (2010). Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges. Revista Brasileira de Farmacognosia, 20, 267-275. Pejin, B, Talevska, A, Ciric, A, Glamoclija, J, Nikolic, M, Talevski, T (2014). Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. Natural Product Research, 28(24), 2330–3.

PY - 2018/3/14

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N2 - The project tested whether freshwater sponge extracts had antimicrobial properties, which affect the growth of nosocomial bacteria. Furthermore, it investigated if antimicrobial properties of the extracts originated from sponges or their microbiome. Two freshwater sponge species Spongilla lacustris and Ephydatia fluviatilis were tested for antimicrobial properties. Sponges of each species were collected from rivers (full microbiome) and laboratory grown in sterile water (reduced microbiome). Sponge samples were dried and extracted in methanol. After evaporation of methanol extracts were resuspended in DMSO. Antimicrobial properties were assessed by disc diffusion and recording the minimal inhibitory zone (MIZ) for the bacteria Acinetobacter baumanniii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis.None of the tested sponge extracts suppressed the growth of P. aeruginosa, S. aureus or S. epidermidis. Only K. pneumoniae was inhibited by all extracts, with the extracts from laboratory grown sponges resulting in significantly wider MIZs (U=73-89, p=0.004-0.019). A. baumanniii, E. coli and E. faecalis were only inhibited by the extract from riverine S. lacustris. Freshwater sponges are potential sources of antimicrobial compounds, particularly against K. pneumoniae. The antimicrobial properties of laboratory grown sponges suggested that the bioactive molecules which inhibited K. pneumoniae originated from the sponge. However, the enhanced antimicrobial properties of S. lacustris from rivers indicated that there was also a contribution by the microbiome to these extracts’ inhibitory efficacy. Successful clinical therapies against nosocomial bacteria with multidrug resistance will increasingly require new antimicrobial compounds. This study has provided evidence that freshwater sponges contain bioactive molecules. The latter do inhibit bacterial growth and therefore could be a source of antimicrobial compounds, which may originate both from the sponges and their microbiome.

AB - The project tested whether freshwater sponge extracts had antimicrobial properties, which affect the growth of nosocomial bacteria. Furthermore, it investigated if antimicrobial properties of the extracts originated from sponges or their microbiome. Two freshwater sponge species Spongilla lacustris and Ephydatia fluviatilis were tested for antimicrobial properties. Sponges of each species were collected from rivers (full microbiome) and laboratory grown in sterile water (reduced microbiome). Sponge samples were dried and extracted in methanol. After evaporation of methanol extracts were resuspended in DMSO. Antimicrobial properties were assessed by disc diffusion and recording the minimal inhibitory zone (MIZ) for the bacteria Acinetobacter baumanniii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis.None of the tested sponge extracts suppressed the growth of P. aeruginosa, S. aureus or S. epidermidis. Only K. pneumoniae was inhibited by all extracts, with the extracts from laboratory grown sponges resulting in significantly wider MIZs (U=73-89, p=0.004-0.019). A. baumanniii, E. coli and E. faecalis were only inhibited by the extract from riverine S. lacustris. Freshwater sponges are potential sources of antimicrobial compounds, particularly against K. pneumoniae. The antimicrobial properties of laboratory grown sponges suggested that the bioactive molecules which inhibited K. pneumoniae originated from the sponge. However, the enhanced antimicrobial properties of S. lacustris from rivers indicated that there was also a contribution by the microbiome to these extracts’ inhibitory efficacy. Successful clinical therapies against nosocomial bacteria with multidrug resistance will increasingly require new antimicrobial compounds. This study has provided evidence that freshwater sponges contain bioactive molecules. The latter do inhibit bacterial growth and therefore could be a source of antimicrobial compounds, which may originate both from the sponges and their microbiome.

KW - Porifera

KW - freshwater sponges

KW - antibiotic resistance

KW - antimicrobial resistance

M3 - Conference contribution

SP - 21

EP - 22

BT - Unknown Host Publication

ER -