Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation

Research output: Contribution to conferencePoster

Abstract

It is acknowledged that the environment facilitates antibiotic resistance development and spread but not much research has been undertaken to understand the mechanisms involved. This study focuses on antibiotic resistance transfer by enterococci, normal inhabitants of the mammalian gut and important healthcare associated pathogens, under a variety of environmental conditions. These are novel environmental strains, isolated from a farm in Monaghan, Ireland. The primary aim of this project is to quantify factors controlling the horizontal gene transfer (HGT) of antibiotic resistance genes via pheromone-induced conjugation. The main objectives include; identifying enterococcal isolates capable of transferring antibiotic resistance genes under laboratory conditions; using whole genome sequencing (WGS) to characterise genetic events involved in conjugal transfer; developing markers of transfer efficiency; and developing environmentally relevant models to measure enterococcal HGT. The transfer of vancomycin resistance genes from a donor to a recipient cell have been demonstrated and transfer efficiencies range from 1.09×10−1 to 9.74×10−5. This data shows that some donors are better at donating vancomycin resistance genes than other donors and some recipients are better than other recipients at receiving them. Preliminary data also shows the transfer of trimethoprim, tetracycline and erythromycin and we can see that within a localised environment Enterococcus faecalis can spread resistance genes very differently. In conclusion this project hopes to further our knowledge of the mechanism of pheromone-induced conjugation in environmental Enterococci and to show that environmental enterococcal strains can become multi-drug resistant just as easily as clinical isolates.

Conference

ConferenceMicrobiology Society Annual Conference 2019
CountryUnited Kingdom
CityBelfast
Period8/04/1911/04/19
Internet address

Fingerprint

Enterococcus faecalis
antibiotic resistance
pheromones
vancomycin
Enterococcus
genes
trimethoprim
erythromycin
tetracycline
Ireland
health services
digestive system
drugs
farms
environmental factors
genome
pathogens
cells
horizontal gene transfer

Cite this

McCarron, Mary ; Dooley, J ; Banat, Ibrahim ; Arnscheidt, Joerg ; Snelling, William J. / Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation. Poster session presented at Microbiology Society Annual Conference 2019, Belfast, United Kingdom.
@conference{3b2fdb5804f04a69a47fe4235f9c4094,
title = "Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation",
abstract = "It is acknowledged that the environment facilitates antibiotic resistance development and spread but not much research has been undertaken to understand the mechanisms involved. This study focuses on antibiotic resistance transfer by enterococci, normal inhabitants of the mammalian gut and important healthcare associated pathogens, under a variety of environmental conditions. These are novel environmental strains, isolated from a farm in Monaghan, Ireland. The primary aim of this project is to quantify factors controlling the horizontal gene transfer (HGT) of antibiotic resistance genes via pheromone-induced conjugation. The main objectives include; identifying enterococcal isolates capable of transferring antibiotic resistance genes under laboratory conditions; using whole genome sequencing (WGS) to characterise genetic events involved in conjugal transfer; developing markers of transfer efficiency; and developing environmentally relevant models to measure enterococcal HGT. The transfer of vancomycin resistance genes from a donor to a recipient cell have been demonstrated and transfer efficiencies range from 1.09×10−1 to 9.74×10−5. This data shows that some donors are better at donating vancomycin resistance genes than other donors and some recipients are better than other recipients at receiving them. Preliminary data also shows the transfer of trimethoprim, tetracycline and erythromycin and we can see that within a localised environment Enterococcus faecalis can spread resistance genes very differently. In conclusion this project hopes to further our knowledge of the mechanism of pheromone-induced conjugation in environmental Enterococci and to show that environmental enterococcal strains can become multi-drug resistant just as easily as clinical isolates.",
author = "Mary McCarron and J Dooley and Ibrahim Banat and Joerg Arnscheidt and Snelling, {William J}",
year = "2019",
month = "4",
day = "8",
doi = "10.1099/acmi.ac2019.po0573",
language = "English",
note = "Microbiology Society Annual Conference 2019 ; Conference date: 08-04-2019 Through 11-04-2019",
url = "https://microbiologysociety.org/event/annual-conference/annual-conference.html",

}

Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation. / McCarron, Mary; Dooley, J; Banat, Ibrahim; Arnscheidt, Joerg; Snelling, William J.

2019. Poster session presented at Microbiology Society Annual Conference 2019, Belfast, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation

AU - McCarron, Mary

AU - Dooley, J

AU - Banat, Ibrahim

AU - Arnscheidt, Joerg

AU - Snelling, William J

PY - 2019/4/8

Y1 - 2019/4/8

N2 - It is acknowledged that the environment facilitates antibiotic resistance development and spread but not much research has been undertaken to understand the mechanisms involved. This study focuses on antibiotic resistance transfer by enterococci, normal inhabitants of the mammalian gut and important healthcare associated pathogens, under a variety of environmental conditions. These are novel environmental strains, isolated from a farm in Monaghan, Ireland. The primary aim of this project is to quantify factors controlling the horizontal gene transfer (HGT) of antibiotic resistance genes via pheromone-induced conjugation. The main objectives include; identifying enterococcal isolates capable of transferring antibiotic resistance genes under laboratory conditions; using whole genome sequencing (WGS) to characterise genetic events involved in conjugal transfer; developing markers of transfer efficiency; and developing environmentally relevant models to measure enterococcal HGT. The transfer of vancomycin resistance genes from a donor to a recipient cell have been demonstrated and transfer efficiencies range from 1.09×10−1 to 9.74×10−5. This data shows that some donors are better at donating vancomycin resistance genes than other donors and some recipients are better than other recipients at receiving them. Preliminary data also shows the transfer of trimethoprim, tetracycline and erythromycin and we can see that within a localised environment Enterococcus faecalis can spread resistance genes very differently. In conclusion this project hopes to further our knowledge of the mechanism of pheromone-induced conjugation in environmental Enterococci and to show that environmental enterococcal strains can become multi-drug resistant just as easily as clinical isolates.

AB - It is acknowledged that the environment facilitates antibiotic resistance development and spread but not much research has been undertaken to understand the mechanisms involved. This study focuses on antibiotic resistance transfer by enterococci, normal inhabitants of the mammalian gut and important healthcare associated pathogens, under a variety of environmental conditions. These are novel environmental strains, isolated from a farm in Monaghan, Ireland. The primary aim of this project is to quantify factors controlling the horizontal gene transfer (HGT) of antibiotic resistance genes via pheromone-induced conjugation. The main objectives include; identifying enterococcal isolates capable of transferring antibiotic resistance genes under laboratory conditions; using whole genome sequencing (WGS) to characterise genetic events involved in conjugal transfer; developing markers of transfer efficiency; and developing environmentally relevant models to measure enterococcal HGT. The transfer of vancomycin resistance genes from a donor to a recipient cell have been demonstrated and transfer efficiencies range from 1.09×10−1 to 9.74×10−5. This data shows that some donors are better at donating vancomycin resistance genes than other donors and some recipients are better than other recipients at receiving them. Preliminary data also shows the transfer of trimethoprim, tetracycline and erythromycin and we can see that within a localised environment Enterococcus faecalis can spread resistance genes very differently. In conclusion this project hopes to further our knowledge of the mechanism of pheromone-induced conjugation in environmental Enterococci and to show that environmental enterococcal strains can become multi-drug resistant just as easily as clinical isolates.

U2 - 10.1099/acmi.ac2019.po0573

DO - 10.1099/acmi.ac2019.po0573

M3 - Poster

ER -

McCarron M, Dooley J, Banat I, Arnscheidt J, Snelling WJ. Antibiotic resistance transfer in Enterococcus faecalis via pheromone-induced conjugation. 2019. Poster session presented at Microbiology Society Annual Conference 2019, Belfast, United Kingdom. https://doi.org/10.1099/acmi.ac2019.po0573