Anti-viral antibody profiling by high density protein arrays.

X Bian, P Wiktor, P Kahn, A Khela, K Karthikeyan, K Barker, X Yu, M Magee, CH Wasserfall, David Gibson, ME Rooney, J Qiu, J LaBaer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Viral infections elicit anti-viral antibodies and have been associated with various chronic diseases. Detection of these antibodies can facilitate diagnosis, treatment of infection and understanding of the mechanisms of virus associated diseases. In this work, we assayed anti-viral antibodies using a novel high density-nucleic acid programmable protein array (HD-NAPPA) platform. Individual viral proteins were expressed in situ directly from plasmids encoding proteins in an array of microscopic reaction chambers. Quality of protein display and serum response was assured by comparing intra- and inter- array correlation within or between printing batches with average correlation coefficients of 0.91 and 0.96, respectively. HD-NAPPA showed higher signal to background (S/B) ratio compared with standard NAPPA on planar glass slides and ELISA. Antibody responses to 761 antigens from 25 different viruses were profiled among patients with juvenile idiopathic arthritis (JIA) and type 1 diabetes (T1D). Common as well as unique antibody reactivity patterns were detected between patients and healthy controls. We believe HD-viral-NAPPA will enable the study of host-pathogen interactions at unprecedented dimensions and elucidate the role of pathogen infections in disease development. This article is protected by copyright. All rights reserved.
LanguageEnglish
Pages2136-2145
JournalProteomics
Volume15
Issue number12
Early online date11 Mar 2015
DOIs
Publication statusE-pub ahead of print - 11 Mar 2015

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Viral Antibodies
Protein Array Analysis
Anti-Idiotypic Antibodies
Virus Diseases
Nucleic Acids
Pathogens
Viruses
Host-Pathogen Interactions
Antibodies
Printing
Proteins
Juvenile Arthritis
Viral Proteins
Infection
Type 1 Diabetes Mellitus
Antibody Formation
Glass
Blood Proteins
Plasmids
Chronic Disease

Cite this

Bian, X., Wiktor, P., Kahn, P., Khela, A., Karthikeyan, K., Barker, K., ... LaBaer, J. (2015). Anti-viral antibody profiling by high density protein arrays. Proteomics, 15(12), 2136-2145. https://doi.org/10.1002/pmic.201400612
Bian, X ; Wiktor, P ; Kahn, P ; Khela, A ; Karthikeyan, K ; Barker, K ; Yu, X ; Magee, M ; Wasserfall, CH ; Gibson, David ; Rooney, ME ; Qiu, J ; LaBaer, J. / Anti-viral antibody profiling by high density protein arrays. In: Proteomics. 2015 ; Vol. 15, No. 12. pp. 2136-2145.
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Bian, X, Wiktor, P, Kahn, P, Khela, A, Karthikeyan, K, Barker, K, Yu, X, Magee, M, Wasserfall, CH, Gibson, D, Rooney, ME, Qiu, J & LaBaer, J 2015, 'Anti-viral antibody profiling by high density protein arrays.', Proteomics, vol. 15, no. 12, pp. 2136-2145. https://doi.org/10.1002/pmic.201400612

Anti-viral antibody profiling by high density protein arrays. / Bian, X; Wiktor, P; Kahn, P; Khela, A; Karthikeyan, K; Barker, K; Yu, X; Magee, M; Wasserfall, CH; Gibson, David; Rooney, ME; Qiu, J; LaBaer, J.

In: Proteomics, Vol. 15, No. 12, 11.03.2015, p. 2136-2145.

Research output: Contribution to journalArticle

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AU - Magee, M

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Bian X, Wiktor P, Kahn P, Khela A, Karthikeyan K, Barker K et al. Anti-viral antibody profiling by high density protein arrays. Proteomics. 2015 Mar 11;15(12):2136-2145. https://doi.org/10.1002/pmic.201400612