Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro

MJ McCann, Chris Gill, G O'Brien, JR Rao, WC McRoberts, P Hughes, R McEntee, IR Rowland

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Abstract

Colorectal cancer is one of the most common cancers in Western countries. The World Health Organisation identifies diet as a critical risk factor in the development and progression of this disease and the protective role of high levels of fruit and vegetable consumption. Several studies have shown that apples contain several phenolic compounds that are potent anti-oxidants in humans. However, little is known about other beneficial properties of apple phenolics in cancer. We have used the HT29, HT 115 and CaCo-2 cell lines as in vitro models to examine the effect of apple phenolics (0.01-0.1% apple extract) on key stages of colorectal carcinogenesis, namely; DNA damage (Comet assay), colonic barrier function (TER assay), cell cycle progression (DNA content assay) and invasion (Matrigel assay). Our results indicate that a crude extract of apple phenolics can protect against DNA damage, improve barrier function and inhibit invasion (p < 0.05). The anti-invasive effects of the extract were enhanced with twenty-four hour pretreatment of cells (P < 0.05). We have shown that a crude apple extract from waste, rich in phenolic compounds, beneficially influences key stages of carcinogenesis in colon cells in vitro. (c) 2007 Elsevier Ltd. All rights reserved.
LanguageEnglish
Pages1224-1230
JournalFood and Chemical Toxicology
Volume45
Issue number7
DOIs
Publication statusPublished - Jul 2007

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carcinogenesis
colon
apples
neoplasms
extracts
assays
DNA damage
phenolic compounds
vegetable consumption
fruit consumption
World Health Organization
colorectal neoplasms
disease course
oxidants
cell cycle
risk factors
pretreatment
cell lines
cells
DNA

Cite this

McCann, MJ., Gill, C., O'Brien, G., Rao, JR., McRoberts, WC., Hughes, P., ... Rowland, IR. (2007). Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro. Food and Chemical Toxicology, 45(7), 1224-1230. https://doi.org/10.1016/j.fct.2007.01.003
McCann, MJ ; Gill, Chris ; O'Brien, G ; Rao, JR ; McRoberts, WC ; Hughes, P ; McEntee, R ; Rowland, IR. / Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro. In: Food and Chemical Toxicology. 2007 ; Vol. 45, No. 7. pp. 1224-1230.
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McCann, MJ, Gill, C, O'Brien, G, Rao, JR, McRoberts, WC, Hughes, P, McEntee, R & Rowland, IR 2007, 'Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro', Food and Chemical Toxicology, vol. 45, no. 7, pp. 1224-1230. https://doi.org/10.1016/j.fct.2007.01.003

Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro. / McCann, MJ; Gill, Chris; O'Brien, G; Rao, JR; McRoberts, WC; Hughes, P; McEntee, R; Rowland, IR.

In: Food and Chemical Toxicology, Vol. 45, No. 7, 07.2007, p. 1224-1230.

Research output: Contribution to journalArticle

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