Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles

P. A. McCarron, R. F. Donnelly, W. Marouf, D. E. Calvert

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Application of non-drug-loaded poly(ethylcyanoacrylate) nanoparticles (NP) to buccal epithelial cells (BEC) imparted both anti-adherent and antifungal effects. NP prepared using emulsion polymerisation and stabilised using cationic, anionic and non-ionic surfactants decreased Candida albicans blastospore adhesion, an effect attributable to the peripheral coating of surfactant. Cetrimide and Pluronic (R) P 123 were shown to be most effective, producing mean percentage reductions in blastospore adherence of 52.7 and 37.0, respectively. Resultant zeta potential matched the polarity of the surfactant, with those stabilised using cetrimide being especially positive (+31.3 mV). Preparation using anionic surfactants was shown to be problematic, with low yield and wide particle size distribution. Evaluation of the antifungal effect of the peripheral coat was evaluated using zones of inhibition and viable counts assays. The former test revealed poor surfactant diffusion through agar, but did show evidence of limited kill. However, the latter method showed that cationic surfactants associated with NP produced high levels of kill, in contrast to those coated with anionic surfactants, where kill was not evident. Non-ionic surfactant-coated NP produced intermediate kill rates. Results demonstrate that surfactant-coated NP, particularly the cationic types, form the possible basis of a prophylactic formulation that primes the candidal target (BEC) against fungal adhesion and infection. (c) 2007 Elsevier B.V. All rights reserved.
LanguageEnglish
Pages182-190
JournalInternational Journal of Pharmaceutics
Volume340
Issue number1-2
DOIs
Publication statusPublished - 25 Mar 2007

Fingerprint

Surface-Active Agents
Nanoparticles
Cheek
Epithelial Cells
poly(ethylcyanoacrylate)
Poloxamer
Mycoses
Emulsions
Candida albicans
Particle Size
Polymerization
Agar

Keywords

  • Candida albicans
  • Blastospores
  • Poly(ethylcyanoacrylate) nanoparticles
  • Adherence
  • Antifungal

Cite this

McCarron, P. A. ; Donnelly, R. F. ; Marouf, W. ; Calvert, D. E. / Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles. 2007 ; Vol. 340, No. 1-2. pp. 182-190.
@article{5b88f2920b824fe186821a6f1d9b5985,
title = "Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles",
abstract = "Application of non-drug-loaded poly(ethylcyanoacrylate) nanoparticles (NP) to buccal epithelial cells (BEC) imparted both anti-adherent and antifungal effects. NP prepared using emulsion polymerisation and stabilised using cationic, anionic and non-ionic surfactants decreased Candida albicans blastospore adhesion, an effect attributable to the peripheral coating of surfactant. Cetrimide and Pluronic (R) P 123 were shown to be most effective, producing mean percentage reductions in blastospore adherence of 52.7 and 37.0, respectively. Resultant zeta potential matched the polarity of the surfactant, with those stabilised using cetrimide being especially positive (+31.3 mV). Preparation using anionic surfactants was shown to be problematic, with low yield and wide particle size distribution. Evaluation of the antifungal effect of the peripheral coat was evaluated using zones of inhibition and viable counts assays. The former test revealed poor surfactant diffusion through agar, but did show evidence of limited kill. However, the latter method showed that cationic surfactants associated with NP produced high levels of kill, in contrast to those coated with anionic surfactants, where kill was not evident. Non-ionic surfactant-coated NP produced intermediate kill rates. Results demonstrate that surfactant-coated NP, particularly the cationic types, form the possible basis of a prophylactic formulation that primes the candidal target (BEC) against fungal adhesion and infection. (c) 2007 Elsevier B.V. All rights reserved.",
keywords = "Candida albicans, Blastospores, Poly(ethylcyanoacrylate) nanoparticles, Adherence, Antifungal",
author = "McCarron, {P. A.} and Donnelly, {R. F.} and W. Marouf and Calvert, {D. E.}",
note = "PT: J; TC: 2",
year = "2007",
month = "3",
day = "25",
doi = "10.1016/j.ijpharm.2007.03.029",
language = "English",
volume = "340",
pages = "182--190",
number = "1-2",

}

Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles. / McCarron, P. A.; Donnelly, R. F.; Marouf, W.; Calvert, D. E.

Vol. 340, No. 1-2, 25.03.2007, p. 182-190.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-adherent and antifungal activities of surfactant-coated poly (ethylcyanoacrylate) nanoparticles

AU - McCarron, P. A.

AU - Donnelly, R. F.

AU - Marouf, W.

AU - Calvert, D. E.

N1 - PT: J; TC: 2

PY - 2007/3/25

Y1 - 2007/3/25

N2 - Application of non-drug-loaded poly(ethylcyanoacrylate) nanoparticles (NP) to buccal epithelial cells (BEC) imparted both anti-adherent and antifungal effects. NP prepared using emulsion polymerisation and stabilised using cationic, anionic and non-ionic surfactants decreased Candida albicans blastospore adhesion, an effect attributable to the peripheral coating of surfactant. Cetrimide and Pluronic (R) P 123 were shown to be most effective, producing mean percentage reductions in blastospore adherence of 52.7 and 37.0, respectively. Resultant zeta potential matched the polarity of the surfactant, with those stabilised using cetrimide being especially positive (+31.3 mV). Preparation using anionic surfactants was shown to be problematic, with low yield and wide particle size distribution. Evaluation of the antifungal effect of the peripheral coat was evaluated using zones of inhibition and viable counts assays. The former test revealed poor surfactant diffusion through agar, but did show evidence of limited kill. However, the latter method showed that cationic surfactants associated with NP produced high levels of kill, in contrast to those coated with anionic surfactants, where kill was not evident. Non-ionic surfactant-coated NP produced intermediate kill rates. Results demonstrate that surfactant-coated NP, particularly the cationic types, form the possible basis of a prophylactic formulation that primes the candidal target (BEC) against fungal adhesion and infection. (c) 2007 Elsevier B.V. All rights reserved.

AB - Application of non-drug-loaded poly(ethylcyanoacrylate) nanoparticles (NP) to buccal epithelial cells (BEC) imparted both anti-adherent and antifungal effects. NP prepared using emulsion polymerisation and stabilised using cationic, anionic and non-ionic surfactants decreased Candida albicans blastospore adhesion, an effect attributable to the peripheral coating of surfactant. Cetrimide and Pluronic (R) P 123 were shown to be most effective, producing mean percentage reductions in blastospore adherence of 52.7 and 37.0, respectively. Resultant zeta potential matched the polarity of the surfactant, with those stabilised using cetrimide being especially positive (+31.3 mV). Preparation using anionic surfactants was shown to be problematic, with low yield and wide particle size distribution. Evaluation of the antifungal effect of the peripheral coat was evaluated using zones of inhibition and viable counts assays. The former test revealed poor surfactant diffusion through agar, but did show evidence of limited kill. However, the latter method showed that cationic surfactants associated with NP produced high levels of kill, in contrast to those coated with anionic surfactants, where kill was not evident. Non-ionic surfactant-coated NP produced intermediate kill rates. Results demonstrate that surfactant-coated NP, particularly the cationic types, form the possible basis of a prophylactic formulation that primes the candidal target (BEC) against fungal adhesion and infection. (c) 2007 Elsevier B.V. All rights reserved.

KW - Candida albicans

KW - Blastospores

KW - Poly(ethylcyanoacrylate) nanoparticles

KW - Adherence

KW - Antifungal

U2 - 10.1016/j.ijpharm.2007.03.029

DO - 10.1016/j.ijpharm.2007.03.029

M3 - Article

VL - 340

SP - 182

EP - 190

IS - 1-2

ER -