An Expanded Multilocus Sequence Typing Scheme for Propionibacterium acnes: Investigation of ‘Pathogenic’, ‘Commensal’ and Antibiotic Resistant Strains

Andrew McDowell, Emma Barnard, István Nagy, Anna Gao, Shuta Tomida, Huiying Li, Anne Eady, Jonathan Cove, Carl E. Nord, Sheila Patrick

    Research output: Contribution to journalArticle

    103 Citations (Scopus)

    Abstract

    The Gram-positive bacterium Propionibacterium acnes is a member of the normal human skin microbiota and is associated with various infections and clinical conditions. There is tentative evidence to suggest that certain lineages may be associated with disease and others with health. We recently described a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes (http://pubmlst.org/pacnes). We now describe an expanded eight gene version based on six housekeeping genes and two ‘putative virulence’ genes (eMLST) that provides improved high resolution typing (91eSTs from 285 isolates), and generates phylogenies congruent with those based on whole genome analysis. When compared with the nine gene MLST scheme developed at the University of Bath, UK, and utilised by researchers at Aarhus University, Denmark, the eMLST method offers greater resolution. Using the scheme, we examined 208 isolates from disparate clinical sources, and 77 isolates from healthy skin. Acne was predominately associated with type IA1 clonal complexes CC1, CC3 and CC4; with eST1 and eST3 lineages being highly represented. In contrast, type IA2 strains were recovered at a rate similar to type IB and II organisms. Ophthalmic infections were predominately associated with type IA1 and IA2 strains, while type IB and II were more frequently recovered from soft tissue and retrieved medical devices. Strains with rRNA mutations conferring resistance to antibiotics used in acne treatment were dominated by eST3, with some evidence for intercontinental spread. In contrast, despite its high association with acne, only a small number of resistant CC1 eSTs were identified. A number of eSTs were only recovered from healthy skin, particularly eSTs representing CC72 (type II) and CC77 (type III). Collectively our data lends support to the view that pathogenic versus truly commensal lineages of P. acnes may exist. This is likely to have important therapeutic and diagnostic implications.
    LanguageEnglish
    Pagese41480
    JournalPLoS ONE
    Volume7
    Issue number7
    DOIs
    Publication statusPublished - 30 Jul 2012

    Fingerprint

    Propionibacterium acnes
    acne
    Multilocus Sequence Typing
    Acne Vulgaris
    Genes
    antibiotics
    Essential Genes
    Anti-Bacterial Agents
    skin (animal)
    Skin
    medical equipment
    Eye Infections
    genes
    Microbiota
    Gram-Positive Bacteria
    Denmark
    Phylogeny
    Microbial Drug Resistance
    Gram-positive bacteria
    Baths

    Cite this

    McDowell, Andrew ; Barnard, Emma ; Nagy, István ; Gao, Anna ; Tomida, Shuta ; Li, Huiying ; Eady, Anne ; Cove, Jonathan ; Nord, Carl E. ; Patrick, Sheila. / An Expanded Multilocus Sequence Typing Scheme for Propionibacterium acnes: Investigation of ‘Pathogenic’, ‘Commensal’ and Antibiotic Resistant Strains. In: PLoS ONE. 2012 ; Vol. 7, No. 7. pp. e41480.
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    abstract = "The Gram-positive bacterium Propionibacterium acnes is a member of the normal human skin microbiota and is associated with various infections and clinical conditions. There is tentative evidence to suggest that certain lineages may be associated with disease and others with health. We recently described a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes (http://pubmlst.org/pacnes). We now describe an expanded eight gene version based on six housekeeping genes and two ‘putative virulence’ genes (eMLST) that provides improved high resolution typing (91eSTs from 285 isolates), and generates phylogenies congruent with those based on whole genome analysis. When compared with the nine gene MLST scheme developed at the University of Bath, UK, and utilised by researchers at Aarhus University, Denmark, the eMLST method offers greater resolution. Using the scheme, we examined 208 isolates from disparate clinical sources, and 77 isolates from healthy skin. Acne was predominately associated with type IA1 clonal complexes CC1, CC3 and CC4; with eST1 and eST3 lineages being highly represented. In contrast, type IA2 strains were recovered at a rate similar to type IB and II organisms. Ophthalmic infections were predominately associated with type IA1 and IA2 strains, while type IB and II were more frequently recovered from soft tissue and retrieved medical devices. Strains with rRNA mutations conferring resistance to antibiotics used in acne treatment were dominated by eST3, with some evidence for intercontinental spread. In contrast, despite its high association with acne, only a small number of resistant CC1 eSTs were identified. A number of eSTs were only recovered from healthy skin, particularly eSTs representing CC72 (type II) and CC77 (type III). Collectively our data lends support to the view that pathogenic versus truly commensal lineages of P. acnes may exist. This is likely to have important therapeutic and diagnostic implications.",
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    An Expanded Multilocus Sequence Typing Scheme for Propionibacterium acnes: Investigation of ‘Pathogenic’, ‘Commensal’ and Antibiotic Resistant Strains. / McDowell, Andrew; Barnard, Emma; Nagy, István; Gao, Anna; Tomida, Shuta; Li, Huiying; Eady, Anne; Cove, Jonathan; Nord, Carl E.; Patrick, Sheila.

    In: PLoS ONE, Vol. 7, No. 7, 30.07.2012, p. e41480.

    Research output: Contribution to journalArticle

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